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Optimization of Lipase-Catalyzed Synthesis of Sorbitan Acrylate Using Response Surface Methodology

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Applied Biochemistry and Biotecnology

Part of the book series: ABAB Symposium ((ABAB))

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Abstract

In this study, we have synthesized sorbitan acrylate through response surface methodology, using sorbitan and vinyl acrylate that catalyze immobilized lipase. In order to optimize the enzymatic synthesis of the sorbitan acrylate, we applied response surface techniques to determine the effects of five-level-four-factors and their reciprocal interactions with the biosynthesis of sorbitan acrylate. Our statistical model predicted that the highest conversion yield of sorbitan acrylate would be approx 100%, under the following optimized reaction conditions: a reaction temperature of 40.1°C, a reaction time of 237.4 min, an enzyme concentration of 8%, and a 4.49∶1 acyl donor/acceptor molar ratio. Using these optimal conditions in three independent replicates, the conversion yield reached 97.6±1.3%.

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Author information

Authors and Affiliations

  1. School of Biological Sciences and Technology, Chonnam National University, Gwangju, 500-757, Korea

    Jeong Gwi-Taek & Park Don-Hee

  2. Engineering Research Institute, Chonnam National University, Gwangju, 500-757, Korea

    Jeong Gwi-Taek

  3. Faculty of Applied Chemical Engineering, Chonnam National University, Gwangju, 500-757, Korea

    Park Don-Hee

  4. Biotechnology Research Institute, Chonnam National University, Gwangju, 500-757, Korea

    Park Don-Hee

  5. Institute of Bioindustrial Technology, Chonnam National University, Gwangju, 500-757, Korea

    Park Don-Hee

  6. Research Institute for Catalysis, Chonnam National University, Gwangju, 500-757, Korea

    Park Don-Hee

Authors
  1. Jeong Gwi-Taek
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  2. Park Don-Hee
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Corresponding author

Correspondence to Park Don-Hee .

Editor information

Editors and Affiliations

  1. Oak Ridge National Laboratory, USA

    Jonathan R. Mielenz

  2. Southern Regional Research Laboratory, USDA-ARS, USA

    K. Thomas Klasson

  3. National Renewable Energy Laboratory, USA

    William S. Adney  & James D. McMillan  & 

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© 2007 Humana Press Inc.

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Gwi-Taek, J., Don-Hee, P. (2007). Optimization of Lipase-Catalyzed Synthesis of Sorbitan Acrylate Using Response Surface Methodology. In: Mielenz, J.R., Klasson, K.T., Adney, W.S., McMillan, J.D. (eds) Applied Biochemistry and Biotecnology. ABAB Symposium. Humana Press. https://doi.org/10.1007/978-1-60327-181-3_49

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