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Optimization of Cyclodextrin Glucanotransferase Production From Bacillus clausii E16 in Submerged Fermentation Using Response Surface Methodology

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Applied Biochemistry and Biotecnology

Part of the book series: ABAB Symposium ((ABAB))

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Abstract

Cyclodextrin glucanotransferase production from Bacillus clausii E16, a new bacteria isolated from Brazilian soil samples was optimized in shake-flask cultures. A 24 full-factorial central composite design was performed to optimize the culture conditions, using a response surface methodology. The combined effect among the soluble starch concentration, the peptone concentration, the yeast extract concentration, and the initial pH value of the culture medium was investigated. The optimum concentrations of the components, determined by a 24 full-factorial central composite design, were 13.4 g/L soluble starch, 4.9 g/L peptone, 5.9 g/L yeast extract, and initial pH 10.1. Under these optimized conditions, the maximum cyclodextrin glucanotransferase activity was. 5.9 U/mL after a 48-h fermentation. This yield was 68% higher than that obtained when the microorganism was cultivated in basal culture medium.

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Author information

Authors and Affiliations

  1. Biochemistry and Applied Microbiology Laboratory, IBILCE/UNESP, São José do Rio Preto, SP, Brazil

    Heloiza Ferreira Alves-Prado, Daniela Alonso Bocchini, Eleni Gomes & Roberto Da Silva

  2. Biology Institute, UNESP, Rio Claro, SP, Brazil

    Heloiza Ferreira Alves-Prado & Jonas Contiero

  3. Chemistry Institute, UNESP, Araraquara, SP, Brazil

    Daniela Alonso Bocchini

  4. Computation Sciences and Statistic Department, IBILCE/UNESP, São José do Rio Preto, SP, Brazil

    Luis Carlos Baida

  5. Biotechnology Department-School of Engineering of Lorena, EEL/USP, CP: 116, Lorena, SP, Brazil

    Inês Conceição Roberto

Authors
  1. Heloiza Ferreira Alves-Prado
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  2. Daniela Alonso Bocchini
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  3. Eleni Gomes
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  4. Luis Carlos Baida
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  5. Jonas Contiero
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  6. Inês Conceição Roberto
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  7. Roberto Da Silva
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Corresponding author

Correspondence to Roberto Da Silva .

Editor information

Editors and Affiliations

  1. Oak Ridge National Laboratory, USA

    Jonathan R. Mielenz

  2. Southern Regional Research Laboratory, USDA-ARS, USA

    K. Thomas Klasson

  3. National Renewable Energy Laboratory, USA

    William S. Adney  & James D. McMillan  & 

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© 2007 Humana Press Inc.

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Alves-Prado, H.F. et al. (2007). Optimization of Cyclodextrin Glucanotransferase Production From Bacillus clausii E16 in Submerged Fermentation Using Response Surface Methodology. In: Mielenz, J.R., Klasson, K.T., Adney, W.S., McMillan, J.D. (eds) Applied Biochemistry and Biotecnology. ABAB Symposium. Humana Press. https://doi.org/10.1007/978-1-60327-181-3_4

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