Abstract
Distillers dried grain with solubles (DDGS) is the major coproduct produced at a dry grind ethanol facility. Currently, it is sold primarily as a ruminant animal feed. DDGS is low cost and relatively high in protein and fiber contents. In this study, DDGS was investigated as carbon source for extracellular hydrolytic enzyme production. Two filamentous fungi, noted for their high cellulolytic and hemicellulolytic enzyme titers, were grown on DDGS: Trichoderma reesei Rut C-30 and Aspergillus niger NRRL 2001. DDGS was either used as delivered from the plant (untreated) or after being pretreated with hot water. Both microorganisms secreted a broad range of enzymes when grown on DDGS. Higher xylanase titers were obtained when cultured on hot water DDGS compared with growth on untreated DDGS. Maximum xylanase titers were produced in 4 d for A. niger and 8 d for T. reesei in shake flask cultures. Larger amounts of enzymes were produced in bioreactors (5L) either equipped with Rushton (for T. reesei) or updraft marine impellers (A. niger). Initial production titers were lower for bioreactor than for flask cultures, especially for T. reesei cultures. Improvement of enzyme titers were obtained using fed-batch feeding schemes.
Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Belyea, R. L., Rausch, K. D., and Tumbleson, M. E. (2004), Biores. Technol. 94, 293–298.
Dien, B. S., Iten, L. B., and Bothast, R. J. (1999), J. Ind. Microbiol. Biotechnol. 22, 575–581.
Wong, K. K. Y, Tan, L. U. L, and Saddler, J. N. (1988), Microbiol. Rev. 52, 305–317.
Kang, S. W., Park, Y. S., Lee, J. S., Hong, S. I., and Kim, S. W. (2004), Biores. Technol. 91, 153–156.
de Vries, R. (2003), Appl. Microbiol. Biotechnol. 61, 10–20.
Schuster, E., Dunn-Coleman, N., Frisvad, J. C., and van Dijck, P. W. M. (2002), Appl. Microbiol. Biotechnol. 59, 426–435.
Mosier, N. S., Hendrickson, R., Brewer, M., Ho, N., Sedlak, M., and Ladisch, M. R. (2005), Biores. Technol. 96(18), 1986–1993.
Mosier, N. S., Hendrickson, R., Brewer, M., et al. (2005), Appl. Biochem. Biotechnol. 125, 1–21.
Mandels, M., Andreotti, R., and Roche, C. (1976), Biotechnol. Bioeng. 6, 17–34.
Miller, G. L. (1959), Anal. Chem. 31, 426–428.
Li, X.-L., Dien, B. S., Cotta, M. A., Wu, Y. V., and Saha B. C. (2005), Appl. Biochem. Biotechnol. 121–124, 321–334.
Oliveira, L. A, Porto, A. L. F., and Tambourgi, E. B. (2006), Biores. Technol. 97, 862–867.
Jiang, Z. Q., Yang, S. Q., Yan, Q. J, Li, L. T., and Tan, S. S. (2005), World J. Microbiol. Biotechnol. 21, 863–867.
Brown, J. A., Cossllin, S. A., and Wood, T. M. (1987), Enzyme Microb. Technol. 9, 355–360.
Smith, D. C. and Wood, T. M. (1991), Biotechnol. Bioeng. 38, 883–890.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2007 Humana Press Inc.
About this chapter
Cite this chapter
Ximenes, E.A., Dien, B.S., Ladisch, M.R., Mosier, N., Cotta, M.A., Li, XL. (2007). Enzyme Production by Industrially Relevant Fungi Cultured on Coproduct From Corn Dry Grind Ethanol Plants. In: Mielenz, J.R., Klasson, K.T., Adney, W.S., McMillan, J.D. (eds) Applied Biochemistry and Biotecnology. ABAB Symposium. Humana Press. https://doi.org/10.1007/978-1-60327-181-3_16
Download citation
DOI: https://doi.org/10.1007/978-1-60327-181-3_16
Publisher Name: Humana Press
Print ISBN: 978-1-60327-180-6
Online ISBN: 978-1-60327-181-3
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)