Skip to main content
SpringerLink
Log in

Biodiesel Fuel Production by the Transesterification Reaction of Soybean Oil Using Immobilized Lipase

  • Chapter
Applied Biochemistry and Biotecnology

Part of the book series: ABAB Symposium ((ABAB))

  • 4128 Accesses

Abstract

The enzymatic alcoholysis of soybean oil with methanol and ethanol was investigated using a commercial, immobilized lipase (Lipozyme RM IM). The effect of alcohol (methanol or ethanol), enzyme concentration, molar ratio of alcohol to soybean oil, solvent, and temperature on biodiesel production was determined. The best conditions were obtained in a solvent-free system with ethanol/oil molar ratio of 3.0, temperature of 50°C, and enzyme concentration of 7.0% (w/w). Three-step batch ethanolysis was most effective for the production of biodiesel. Ethyl esters yield was about 60% after 4 h of reaction.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 249.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Srivastava, A. and Prasad, R. (2000), Renew. Sustain Energy Rev. 4, 111–133.

    Article  CAS  Google Scholar 

  2. Shieh, C. J., Liao, H. F., and Lee, C. C. (2003), Bioresour. Technol. 88, 103–106.

    Article  CAS  Google Scholar 

  3. Du, W., Xu, Y., Liu, D., and Zeng, J. (2004), J. Mol. Catal. B: Enzym. 30, 125–129.

    Article  CAS  Google Scholar 

  4. Gerpen, J. V. (2005), Fuel Process. Technol. 86, 1097–1107.

    Article  Google Scholar 

  5. Meher, L. C., Sagar, D. V., and Naik, S. N. (2004), Renew. Sustain. Energy Rev. 20, 1–21.

    Google Scholar 

  6. Barnwal, B. K. and Sharma, M. P. (2005), Renew. Sustain. Energy Rev. 9, 363–378.

    Article  Google Scholar 

  7. Shimada, Y., Watanabe, Y., Sugihara, A., and Toninaga, Y. (2002), J. Mol. Catal. B: Enzym. 17, 133–142.

    Article  CAS  Google Scholar 

  8. Iso, M., Chen, B., Eguchi, M., Kudo, T., and Shrestha, S. (2001), J. Mol. Catal. B: Enzym. 16, 53–58.

    Article  CAS  Google Scholar 

  9. Soumanou, M. M. and Bornscheuer, U. T. (2003), Enzyme Microb. Technol. 33, 97–103.

    Article  CAS  Google Scholar 

  10. Illanes, A. (1994), Biotecnologia de Enzimas, Ediciones Universitarias de Valparaíso de la Universidad Católica de Valparaíso, Chile: pp. 235–243.

    Google Scholar 

  11. Koskinen, A. M. D. and Klibanov, A. M. (1996), Enzymatic Reactions in Organic Media, 1st ed., Blackie Academic & Professional, Bishopbriggs, UK: pp. 10–14, 54–57

    Google Scholar 

  12. Köse, Ö., Tüter, M., and Aksoy, H. A. (2002), Bioresour. Technol. 83, 125–129.

    Article  Google Scholar 

  13. Lima, F. V., Pyle, D. L., and Ansejo, J. A. (1995), Biotechnol. Bioeng. 46, 69–79.

    Article  CAS  Google Scholar 

  14. Xu, Y., Du, W., and Liu, D. (2005), J. Mol. Catal. B: Enzyme 32, 241–245.

    Article  CAS  Google Scholar 

  15. Dossat, V., Combes, D., and Marty, A. (2002), J. Biotechnol. 97, 117–124.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Instituto de Química, Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier, 524, PHLC, sl. 427, CEP: 20559-900, RJ, RJ, Brazil

    Otávio L. Bernardes & Marta A. P. Langone

  2. Centro de Pesquisa e Desenvolvimento da Petrobras-Cenpes, Brazil

    Juliana V. Bevilaqua

  3. Instituto de Química, Universidade Federal do Rio de Janeiro, RJ, CEP: 21949-900, Brazil

    Márcia C. M. R. Leal & Denise M. G. Freire

Authors
  1. Otávio L. Bernardes
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Juliana V. Bevilaqua
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Márcia C. M. R. Leal
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Denise M. G. Freire
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Marta A. P. Langone
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Marta A. P. Langone .

Editor information

Editors and Affiliations

  1. Oak Ridge National Laboratory, USA

    Jonathan R. Mielenz

  2. Southern Regional Research Laboratory, USDA-ARS, USA

    K. Thomas Klasson

  3. National Renewable Energy Laboratory, USA

    William S. Adney  & James D. McMillan  & 

Rights and permissions

Reprints and permissions

Copyright information

© 2007 Humana Press Inc.

About this chapter

Cite this chapter

Bernardes, O.L., Bevilaqua, J.V., Leal, M.C.M.R., Freire, D.M.G., Langone, M.A.P. (2007). Biodiesel Fuel Production by the Transesterification Reaction of Soybean Oil Using Immobilized Lipase. In: Mielenz, J.R., Klasson, K.T., Adney, W.S., McMillan, J.D. (eds) Applied Biochemistry and Biotecnology. ABAB Symposium. Humana Press. https://doi.org/10.1007/978-1-60327-181-3_10

Download citation

Publish with us

Policies and ethics

Search

Navigation