Biological Phosphorus Removal Processes

  • Yong-Qiang Liu
  • Yu Liu
  • Joo-Hwa Tay
  • Yung-Tse Hung
Part of the Handbook of Environmental Engineering book series (HEE, volume 11)


Enhanced biological phosphorus removal (EBPR) processes developed for wastewater treatment are mainly based on the enrichment of activated sludge with phosphorus-accumulating organisms under alternative anaerobic–aerobic conditions. According to the literature information of the EBPR processes, this chapter attempts to review the biochemical models, microbiology of the EBPR processes, and the main operating parameters that may influence the performance of the EBPR processes.


Activate Sludge Terminal Restriction Fragment Length Polymorphism Sequencing Batch Reactor Phosphorus Removal Aerobic Granule 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Christen K (2007) Phosphorus removal: how low can we go? Environ Sci Technol 41:674–674Google Scholar
  2. 2.
    Kortstee GJJ, Appeldorn KJ, Bonting CFC, van Niel EWJ, van Veen HW (1994) Biology of phosphate-accumulating bacteria involved in enhanced biological phosphorus removal. FEMS Microbiol Rev 15:137–153CrossRefGoogle Scholar
  3. 3.
    Nixon SW, Ammerman JW, Atkinson LP, Berounsky VM, Billen G, Boicourt WC, Boynton WR, Church TM, Ditoro DM, Elmgren R, Garber JH, Giblin AE, Jahnke RA, Owens NJP, Pilson MEQ, Seitzinger SP (1996) The fate of nitrogen and phosphorus at the land sea margin of the north Atlantic ocean. Biogeochemistry 35:141–180CrossRefGoogle Scholar
  4. 4.
    Morse GK, Brett SW, Lester JN (1998) Review: phosphorus removal and recovery technologies. Sci Total Environ 212:69–81CrossRefGoogle Scholar
  5. 5.
    Osee Muyima NY, Momba MNB, Cloete TE (1997) Biological methods for the treatment of wastewaters. In: Cloete TE, Moyima NYO (eds) Microbial community analysis: the key to the design of biological wastewater treatment systems. IAWQ Publishers, London, pp 1–24Google Scholar
  6. 6.
    Brdjanovic D, Slamet A, van Loosdrecht MCM, Hooijmans CM, Alaerts GJ, Heijnen JJ (1998) Impact of excessive aeration on biological phosphorus removal from wastewater. Water Res 32:200–208CrossRefGoogle Scholar
  7. 7.
    Wagner M, Loy A (2002) Bacterial community composition and function in sewage treatment systems. Curr Opin Biotechnol 13:218–227CrossRefGoogle Scholar
  8. 8.
    Fuhs GW, Chen M (1975) Microbiological basis of phosphate removal in the activated sludge process for the treatment of wastewater. Microb Ecol 2:119–138CrossRefGoogle Scholar
  9. 9.
    Wentzel MC, Lotter LH, Loewenthal RE, Marais GvR (1986) Metabolic behaviour of Acinetobacter spp. in enhanced biological phosphorus removal – a biochemical model. Water SA 12:209–224Google Scholar
  10. 10.
    Greenburg AE, Levin G, Kauffman WJ (1955) Effect of phosphorus removal on the activated sludge process. Sewage Ind Waste 27:227Google Scholar
  11. 11.
    Srinath EG, Sastry CA, Pillai SC (1959) Rapid removal of phosphorus from sewage by activated sludge. Experientia 15:339–345CrossRefGoogle Scholar
  12. 12.
    Levin GV, Shapiro J (1965) Metabolic uptake of phosphorus by wastewater organisms. J Water Pollut Control Fed 37:800–821Google Scholar
  13. 13.
    Comeau Y, Hall KJ, Hancock REW, Oldham WK (1986) Biochemical model for enhanced biological phosphorus removal. Water Res 20:1511–1521CrossRefGoogle Scholar
  14. 14.
    Matsuo Y (1985) Functioning of the TCA cycle under anaerobic conditions in the anaerobic aerobic acclimated activated sludge. Proc 40th Annu Conf Jpn Soc Civil Eng 40:989–990Google Scholar
  15. 15.
    van Groenestijn JW, Deinema MH, Zehnder AJB (1987) ATP production from polyphosphate in Acinetobacter strain 210A. Arch Microbiol 148:14–19CrossRefGoogle Scholar
  16. 16.
    Mino T, Arun V, Tsuzuki Y, Matsuo T (1987) Effect of phosphorus accumulation on acetate metabolism in the biological phosphorus removal process. In: Ramadori R (ed) Biological phosphorus removal from wastewaters. Advances in water pollution control. Pergamon, Oxford, pp 27–38Google Scholar
  17. 17.
    Osborn DW, Lotter LH, Pitman AR, Nicholls HA (1989) Two-year study on the enhancement of biological phosphate removal by altering process feed composition (Plant and Laboratory Studies). WRC Report No. 137/2/89Google Scholar
  18. 18.
    Bordacs K, Chiesa SC (1989) Carbon flow patterns in enhanced biological phosphorus accumulating activated sludge cultures. Water Sci Technol 21:387–396Google Scholar
  19. 19.
    Satoh H, Mino T, Matsuo T (1992) Uptake of organic substrates and accumulation of polyhydroxyalkanoates linked with glycolysis of intracellular carbohydrates under anaerobic conditions in the biological excess phosphate removal process. Water Sci Technol 26:933–942Google Scholar
  20. 20.
    Maurer M (1997) Intracelluar carbon flow in phosphorus accumulating organisms from activated sludge systems. Water Res 31:907–917CrossRefGoogle Scholar
  21. 21.
    Pereira H, Lemos PC, Reis MAM, Crespo JPSG, Carrondo MJT, Santos H (1996) Model for carbon metabolism in biological phosphorus removal processes based on in vivo 13C-NMR labelling experiments. Water Res 30:2128–2138CrossRefGoogle Scholar
  22. 22.
    Hesselmann RPX, von Rummel R, Resnick SM, Hany R, Zehnder AJB (2000) Anaerobic metabolism of bacteria performing enhanced biological phosphate removal. Water Res 34:3487–3494CrossRefGoogle Scholar
  23. 23.
    Mino T, van Loosdrecht MCM, Heijnen JJ (1998) Microbiology and biochemistry of the enhanced biological phosphate removal process. Water Res 32:3193–3207CrossRefGoogle Scholar
  24. 24.
    Dawes EA, Senior PJ (1973) The role and regulation of energy reserve polymers in microorganisms. Adv Microb Physiol 10:135–266CrossRefGoogle Scholar
  25. 25.
    Seviour RJ, Mino T, Onuki M (2003) The microbiology of biological phosphorus removal in activated sludge systems. FEMS Microbiol Rev 27:99–127CrossRefGoogle Scholar
  26. 26.
    Wentzel MC, Lotter LH, Ekama GA, Loewenthal TE, Marais GvR (1991) Evaluation of biochemical models for biological excess phosphorus removal. Water Sci Technol 23:567–576Google Scholar
  27. 27.
    Buchan L (1983) The possible biological mechanism of phosphorus removal. Water Sci Technol 15:87–103Google Scholar
  28. 28.
    Lotter LH, Murphy M (1985) Identification of heterotrophic bacteria in an activated sludge plant with particular reference to polyphosphate accumulation. Water SA 11:179–184Google Scholar
  29. 29.
    Wentzel MC, Loewenthal RE, Ekama GA, Marais GvR (1988) Enhanced polyphosphate organism cultures in activated sludge systems-part 1: enhanced culture development. Water SA 14:81–92Google Scholar
  30. 30.
    Deinema MH, van Loosdrecht M, Scholten A (1985) Some physiological characteristics of Acinetobacter spp. accumulating large amounts of phosphate. Water Sci Technol 17:119–125Google Scholar
  31. 31.
    Streichan M, Golecki JR, Schoen G (1990) Polyphosphate accumulating bacteria from sewage plants with different processes for biological phosphorus removal. FEMS Microbiol Ecol 73: 113–124CrossRefGoogle Scholar
  32. 32.
    Beacham AM, Seviour RJ, Lindrea KC (1992) Polyphosphate accumulating abilities of Acinetobacter isolates from a biological nutrient removal pilot plant. Water Res 26:121–122CrossRefGoogle Scholar
  33. 33.
    Stante L, Cellamare CM, Malaspina F, Bortone G, Tilche A (1997) Biological phosphorus removal by pure culture of Lampropedia spp. Water Res 31:1317–1324CrossRefGoogle Scholar
  34. 34.
    Nakamura K, Hiraishi A, Yoshimi Y, Kawaharasaki M, Masuda K, Kamagata Y (1995) Microlunatus phosphovorus gen-nov, sp-nov, a new gram-positive polyphosphate-accumulating bacterium isolating from activated sludge. Int J Syst Bacteriol 45:17–22CrossRefGoogle Scholar
  35. 35.
    Shintani T, Liu WT, Hanada S, Kamagata Y, Miyaoka S, Suzuki T, Nakamura K (2000) Micropruina glycogenica gen. nov., sp, nov., a new Gram-positive glycogen-accumulating bacterium isolated from activated sludge. Int J Syst Evol Microbiol 50:201–207CrossRefGoogle Scholar
  36. 36.
    Maszenan AM, Seviour RJ, Patel BKC, Schumann P, Burghardt J, Tokiwa Y, Stratton HM (2000) Three isolates of novel polyphosphate-accumulating Gram-positive cocci, obtained from activated sludge, belong to a new genus, Tetrasphaera gen. nov., and description of two new species, Tetrasphaera japonica sp, nov and Tetrasphaera australiensis sp nov. Int J Syst Evol Microbiol 50:593–603CrossRefGoogle Scholar
  37. 37.
    Jenkins D, Tandoi V (1991) The applied microbiology of enhanced biological phosphate removal – accomplishments and needs. Water Res 25:1471–1478CrossRefGoogle Scholar
  38. 38.
    Hiraishi A, Masamune K, Kitamura H (1989) Characterization of the bacterial population structure in an anaerobic-aerobic activated sludge system on the basis of respiratory quinine profiles. Appl Environ Microbiol 30:197–210Google Scholar
  39. 39.
    Amann RI, Krumholz L, Stahl DA (1990) Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology. J Bacteriol 172: 762–770Google Scholar
  40. 40.
    Muyzer G, de Waal EC, Uitterlinden AG (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 59:695–700Google Scholar
  41. 41.
    Liu WT, Marsh TL, Cheng H, Forney LJ (1997) Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA. Appl Environ Microbiol 63:4516–4522Google Scholar
  42. 42.
    Auling G, Pilz F, Busse HJ, Karrasch M, Streichan M, Schon G (1991) Analysis of the polyphosphate-accumulating microflora in phosphorus-eliminating, anaerobic-aerobic activated sludge systems by using diaminopropane as a biomarker for rapid estimation of Acinetobacter spp. Appl Environ Microbiol 57:3685–3692Google Scholar
  43. 43.
    Hiraishi A, Ueda Y, Ishihara J (1998) Quinone profling of bacterial communities in natural and synthetic sewage activated sludge for enhanced phosphate removal. Appl Environ Microbiol 64:992–998Google Scholar
  44. 44.
    Fujita M, Chen H, Furamai H (1999) An investigation on microbial population dynamics in enhanced biological phosphorus removal SBR using quinone profile and PCR-DGGE techniques In: Proceedings of 7th IAWQ Asian Pacific Conference, TaipaiGoogle Scholar
  45. 45.
    Cloete TE, Steyn PL (1987) A combined fluorescent antibody-membrane filter technique for enumerating Acinetobacter in activated sludge. In: Ramadori R (ed) Proc. IAWPRC Int. Conf. in Rome on method “Biological Phosphate Removal from Wastewaters”, Advances in water pollution control. Pergamon Press, Rome, pp 335–338Google Scholar
  46. 46.
    Hiraishi A, Morishita Y (1990) Capacity for polyphosphate accumulation of predominant bacteria in activated sludge showing enhanced phosphate removal. J Ferment Bioeng 69:368–371CrossRefGoogle Scholar
  47. 47.
    Wagner M, Amann R, Lemmer H, Manz W, Schleifer KH (1994) Probing activated sludge with fluorescently labeled rRNA targeted oligonucleotides. Water Sci Technol 29:15–23Google Scholar
  48. 48.
    Bond PL, Keller J, Blackall LL (1998) Characterisation of enhanced biological phosphorus removal activated sludges with dissimilar phosphorus removal performances. Water Sci Technol 37:567–571CrossRefGoogle Scholar
  49. 49.
    Bond PL, Hugenholtz P, Keller J, Blackall LL (1995) Bacterial community structures of phosphate-removing and non-phosphate-removing activated sludge from sequencing batch reactors. Appl Environ Microbiol 61:1910–1916Google Scholar
  50. 50.
    Snaidr J, Amann R, Huber I, Ludwig W (1997) Phylogenetic analysis and in situ identification of bacteria in activated sludge. Appl Environ Microbiol 63:2884–2896Google Scholar
  51. 51.
    Wagner M, Amann R, Lemmer H, Schleifer KH (1993) Probing activated sludge with oligonucleotides specific for proteobacteria: inadequacy of culture-dependent methods for describing microbial community structure. Appl Environ Microbiol 59:1520–1525Google Scholar
  52. 52.
    Sudiana IM, Mino T, Satoh H, Matsuo T (1998) Morphology, in-situ characterization with rRNA targeted probes and respiratory quinone profiles of enhanced biological phosphorus removal sludge. Water Sci Technol 38:69–76Google Scholar
  53. 53.
    Bond PL, Erhart R, Wagner M, Keller J, Blackall LL (1999) Identification of some of the major groups of bacteria in efficient and nonefficient biological phosphorus removal activated sludge systems. Appl Environ Microbiol 65:4077–4084Google Scholar
  54. 54.
    Olsen GJ, Lane DJ, Giovannoni SJ, Pace NR, Stahl DA (1986) Microbial ecology and evolution: a ribosomal RNA approach. Annu Rev Microbiol 40:337–365CrossRefGoogle Scholar
  55. 55.
    Hesselmann RPX, Werlen C, Hahn D, van der Meer JR, Zehnder AJB (1999) Enrichment, phylogenetic analysis and detection of a bacterium that performs enhanced biological phosphate removal in activated sludge. Syst Appl Microbiol 22:454–465CrossRefGoogle Scholar
  56. 56.
    Crocetti GR, Hugenholtz P, Bond PL, Schuler A, Keller J, Jenkins D, Blackall LL (2000) Identification of polyphosphate-accumulating organisms and design of 16S rRNA-directed probes for their detection and quantitation. Appl Environ Microbiol 66:1175–1182CrossRefGoogle Scholar
  57. 57.
    Dabert P, Fleura-Lessard A, Mounier E, Delgenes JP, Moletta R, Godon JJ (2001) Monitoring of the microbial community of a sequencing batch reactor bioaugmented to improve its phosphorus removal capabilities. Water Sci Technol 43:1–8Google Scholar
  58. 58.
    Dabert P, Sialve B, Delgenes JP, Moletta R, Godon JJ (2001) Characterisation of the microbial 16S rDNA diversity of an aerobic phosphorus-removal ecosystem and monitoring of its transition to nitrate respiration. Appl Microbiol Biotech 55:500–509CrossRefGoogle Scholar
  59. 59.
    Onuki M, Satoh H, Mino T (2001) Analysis of microbial community that performs enhanced biological phosphorus removal in activaded sludge fed with acetate. In: Tandoi V, Passino R, Blundo CM (eds) Microorganisms in activated sludge and biofilm processes. Proceedings of the 3rd International Water Association Conference. International Water Association, London, England, pp 98–105Google Scholar
  60. 60.
    Zilles JL, Hung C-H, Noguera DR (2001) Presence of Rhodocyclus in a full-scale wastewater treatment plant and their participation in enhanced biological phosphorous removal. In: Tandoi V, Passino R, Blundo CM (eds) Microorganisms in activated sludge and biofilm processes. Proceedings of the 3rd International Water Association Conference. International Water Association, London, England, pp 75–81Google Scholar
  61. 61.
    Kawaharasaki M, Tanaka H, Kanagawa T, Nakamura K (1999) In situ identification of polyphosphate-accumulating bacteria in activated sludge by dual staining with rRNA-targeted oligonucleotide probes and 4’, 6-diamidino-2-phenylindol (DAPI) at a polyphosphate-probing concentration. Water Res 33:257–265CrossRefGoogle Scholar
  62. 62.
    Liu WT, Nielsen AT, Wu JH, Tsai CS, Matsuo Y, Molin S (2001) In situ identification of polyphosphate- and polyhydroxyalkanoate-accumulating traits for microbial populations in a biological phosphorus removal process. Environ Microbiol 3:110–122CrossRefGoogle Scholar
  63. 63.
    Cech JB, Hartman P (1990) Glucose induced breakdown of enhanced biological phosphate removal. Environ Technol 11:651–656CrossRefGoogle Scholar
  64. 64.
    Cech JB, Hartman P (1993) Competition between polyphosphate and polysaccharide accumulating bacteria in enhanced biological phosphate removal systems. Water Res 27:1219–1225CrossRefGoogle Scholar
  65. 65.
    Shapiro J, Levin GV, Humberto ZG (1967) Anoxically induced release of phosphate in sewage treatment. J Water Pollut Control Fed 39:1810–1818Google Scholar
  66. 66.
    Barnard JL (1974) Cut P and N without chemicals. Water Waste Eng 11:33–36Google Scholar
  67. 67.
    Sedlak RI (1991) Phosphorus and nitrogen removal from municipal wastewater. Lewis, New YorkGoogle Scholar
  68. 68.
    Krichten DJ, Nicholas DM, Galdiers JV (1978) Phosphorus and BOD removal in an activated sludge system without chemical addition. Presented at the 176th national meeting, American chemical society, Miami Beach, FLGoogle Scholar
  69. 69.
    Hong SN, Krichten DJ, Kisenbauer KS, Sell RL (1982) A biological wastewater treatment system for nutrient removal. Presented at the workshop on biological phosphorus removal in municipal wastewater treatment, Annapolis, MDGoogle Scholar
  70. 70.
    Metcalf & Eddy (2003) Wastewater engineering, treatment, disposal and reuse. New York: McGraw-HillGoogle Scholar
  71. 71.
    Lemaire R, Yuan ZG, Bernet N, Marcos M, Yilmaz G, Keller J (2009) A sequencing batch reactor system for high-level biological nitrogen and phosphorus removal from abattoir wastewater. Biodegradation 20:339–350CrossRefGoogle Scholar
  72. 72.
    Beun JJ, Hendriks A, Loosdrecht MCM, van Morgenroth E, Wilderer PA, Heijnen JJ (1999) Aerobic granulation in a sequencing batch reactor. Water Res 33:2283–2290CrossRefGoogle Scholar
  73. 73.
    Shi XY, Yu HQ, Sun YJ, Huang X (2009) Characteristics of aerobic granules rich in autotrophic ammonium-oxidizing bacteria in a sequencing batch reactor. Chem Eng J 147:102–109CrossRefGoogle Scholar
  74. 74.
    Adav SS, Lee DJ, Show KY, Tay JH (2008) Aerobic granular sludge: Recent advances. Biotechnol Adv 26:411–423CrossRefGoogle Scholar
  75. 75.
    Lin YM, Liu Y, Tay JH (2003) Development and characteristics of phosphorus-accumulating microbial granules in sequencing batch reactors. Appl Microbiol Biotechnol 62:430–435CrossRefGoogle Scholar
  76. 76.
    Liu Y, Lin YM, Tay JH (2005) The elemental compositions of P-accumulating microbial granules developed in sequencing batch reactors. Process Biochem 40:3258–3262CrossRefGoogle Scholar
  77. 77.
    Cassidy DP, Belia E (2005) Nitrogen and phosphorus removal from an abattoir wastewater in a SBR with aerobic granular sludge. Water Res 19:4817–4823CrossRefGoogle Scholar
  78. 78.
    Kishida N, Kim J, Tsuneda S, Sudo R (2006) Anaerobic/oxic/anoxic granular sludge process as an effective nutrient removal process utilizing denitrifying polyphosphate-accumulating organisms. Water Res 40:2303–2310CrossRefGoogle Scholar
  79. 79.
    de Kreuk MK, Pronk M, van Loosdrecht MCM (2005) Formation of aerobic granules and conversion processes in an aerobic granular sludge reactor at moderate and low temperatures. Water Res 39:4476–4484CrossRefGoogle Scholar
  80. 80.
    de Kreuk M, Heijnen JJ, van Loosdrecht MCM (2005) Simultaneous COD, nitrogen, and phosphate removal by aerobic granular sludge. Biotechnol Bioeng 90:761–769CrossRefGoogle Scholar
  81. 81.
    Gerber A, Simpkins MJ, Winter CT, Scheepers JA (1982) Biological nutrient removal from wastewater effluents: Performance Evaluation of the Full PHOREDOX and UCT processes. Contract report 52, council for scitific and industrial research, Pretoria, South AfricaGoogle Scholar
  82. 82.
    Barnard JL, Stevens GM, Leslie PJ (1985) Design strategies for nutrient removal plants. Water Sci Technol 17:233–242Google Scholar
  83. 83.
    Fukase T, Shibata M, Miyaji Y (1985) The role of an anaerobic stage on the biological phosphorus removal. Water Sci Technol 17:69–80Google Scholar
  84. 84.
    Pitman AR, Trim BC, van Dalsen L (1988) Operating experience with biological nutrient removal at the Hohannesburg Bushkoppie works. Water Sci Technol 20:51–61Google Scholar
  85. 85.
    Wentzel MC, Ekama GA, Marais GvR (1992) Processes and modeling of nitrification denitrification biological excess phosphorus removal systems – a review. Water Sci Technol 25:59–82Google Scholar
  86. 86.
    Tam NFY, Wong YS, Leung G (1992) Significance of external carbon sources on simultaneous removal of nutrients from wastewater. Water Sci Technol 26:1047–1055Google Scholar
  87. 87.
    Randall AA, Benefield LD, Hill WE (1994) Effect of fermentation products on enhanced biological phosphorus removal, polyphosphate storage, and microbial population dynamics. Water Sci Technol 30:213–219Google Scholar
  88. 88.
    Satoh H, Mino T, Matsuo T (1994) Deterioration of enhanced biological phosphorus removal by the domination of microorganisms without poly-phosphate accumulation. Water Sci Technol 30:203–211Google Scholar
  89. 89.
    Martinez AD, Canizares PC, Mayor LR, Camacho JV (2001) Short-term effects of wastewater biodegradability on biological phosphorus removal. J Environ Eng 127:259–265CrossRefGoogle Scholar
  90. 90.
    Ruel SM, Comeau Y, Heduit A, Deronzier G, Ginestet P, Audic JM (2002) Operating conditions for the determination of the biochemical acidogenic potential of wastewater. Water Res 36:2337–2341CrossRefGoogle Scholar
  91. 91.
    Randall AA, Benefield LD, Hill WE, Nicol JP, Boman GK, Jing SR (1997) The effect of volatile fatty acids on enhanced biological phosphorus removal and population structure in anaerobic/aerobic sequencing batch reactors. Water Sci Technol 35:153–160Google Scholar
  92. 92.
    Hood C, Randall AA (2001) A biochemical hypothesis explaining the response of enhanced biological phosphorus removal biomass to organic substrates. Water Res 35:2758–2766CrossRefGoogle Scholar
  93. 93.
    Rustrian E, Delgenes JP, Moletta R (1996) Effect of the volatile fatty acids on phosphate uptake parameters by pure cultures of Acinetobacter spp. Lett Appl Microbiol 23:245–248CrossRefGoogle Scholar
  94. 94.
    Barnard JL (1993) Prefermentation in biological nutrient removal plants. Proceedings of the Joint CSCE-ASCE National Conference on Environmental Engineering, Montreal, Quebec, Canada, 12–14 July 1993, pp 1767–1774Google Scholar
  95. 95.
    Maharaj I, Elefsiniotis P (2001) The role of HRT and low temperature on the acidphase anaerobic digestion of municipal and industrial wastewaters. Bioresour Technol 76:191–197CrossRefGoogle Scholar
  96. 96.
    Randall CW, Chapin RW (1997) Acetic acid inhibition of biological phosphorus removal. Water Environ Res 69:955–960CrossRefGoogle Scholar
  97. 97.
    Morgenroth E, Wilderer PA (1998) Modeling of enhanced biological phosphorus removal in a sequencing batch biofilm reactor. Water Sci Technol 37:583–587CrossRefGoogle Scholar
  98. 98.
    Liu WT, Mino T, Matsuo T, Nakamura K (1996) Biological phosphorus removal process – effect of pH on anaerobic substrate metabolism. Water Sci Technol 34:25–32Google Scholar
  99. 99.
    Randall CW, Barnard JL, Stensel HD (1992) Design and retrofit of wastewater treatment plants for biological nutrient removal. Technomic Publishing, Lancaster, pp 25–78Google Scholar
  100. 100.
    Romanski J, Heider M, Wiesmann U (1997) Kinetics of anaerobic orthophosphate release and substrate uptake in enhanced biological phosphorus removal from synthetic wastewater. Water Res 31:3137–3145CrossRefGoogle Scholar
  101. 101.
    Murthy SN, Novak JT (1998) Effects of potassium ion on sludge settling, dewatering and effluent properties. Water Sci Technol 37:317–324Google Scholar
  102. 102.
    Barnard JL (1976) A review of biological phosphorus removal in the activated sludge process. Water SA 2:136–144Google Scholar
  103. 103.
    Choi YS, Shin EB, Lee YD (1996) Biological phosphorus removal from wastewater in a single reactor combining anaerobic and aerobic conditions. Water Sci Technol 34:179–186Google Scholar
  104. 104.
    Rabinowitz B (1985) The role of specific substrates in excess biological phosphorus removal. Ph.D. Thesis, the University British Columbia, Vancouver, British Columbia, CanadaGoogle Scholar
  105. 105.
    Rensink JH, van der Ven J, van Pamelen G, Fedder F, Majoor E (1997) The modified Renphosystem: a high biological nutrient removal system. Water Sci Technol 35:137–146Google Scholar
  106. 106.
    Liu WT, Marsh TL, Forney LJ (1998) Determination of the microbial diversity of anaerobic – aerobic activated sludge by a novel molecular biological technique. Water Sci Technol 37: 417–422CrossRefGoogle Scholar
  107. 107.
    Randall AA, Benefield LD, Hill WE (1997). Induction of phosphorus removal in an enhanced biological phosphorus removal bacterial population. Water Res 31:2869–2877CrossRefGoogle Scholar
  108. 108.
    Sudiana IM, Mino T, Satoh H, Nakamura K, Matsuo T (1999) Metabolism of enhanced biological phosphorus removal and non-enhanced phosphorus removal sludge with acetate and glucose as carbon source. Water Sci Technol 39:29–35Google Scholar
  109. 109.
    Converti A, Rovatti M, Del Borghi M (1995) Biological removal of phosphorus from wastewaters by alternating aerobic and anaerobic conditions. Water Res 29:263–269CrossRefGoogle Scholar
  110. 110.
    Brdjanovic D, van Loosdrecht MCM, Hooijmans CM, Alaerts GJ, Heijnen JJ (1997) Temperature effects on physiology of biological phosphorus removal. J Environ Eng 123:144–153CrossRefGoogle Scholar
  111. 111.
    Panswad T, Doungchai A, Anotai J (2003) Temperature effect on microbial community of enhanced biological phosphorus removal system. Water Res 37:409–415CrossRefGoogle Scholar
  112. 112.
    Groenestijn JW, Deinema MH (1985) Effects of cultural conditions on phosphate accumulation and release by Acinetobactor strain 210A. Proceeding of the international conference, management strategies for phosphorus in the environment, Lisbon, Portugal, July 1–4, 1985Google Scholar
  113. 113.
    Tracy KD, Flammino A (1985) Kinetics of biological phosphorus removal. Presented at the 58th annual water pollution control federation conference, Kansas city, Missouri, October 1985Google Scholar
  114. 114.
    Alleman JE (1984) Elevated nitrite occurrence in biological wastewater treatment systems. Water Sci Technol 17:409–419Google Scholar
  115. 115.
    Louzeiro NR, Mavinic DS, Oldham WK, Meisen A, Gardner IS (2002) Methanolinduced biological nutrient removal kinetics in a full-scale sequencing batch reactor. Water Res 36:2721–2732CrossRefGoogle Scholar
  116. 116.
    Shehab O, Deininger R, Porta F, Wojewski T (1996) Optimising phosphorus removal at the Ann Arbor wastewater treatment plant. Water Sci Technol 34:493–499Google Scholar
  117. 117.
    Matsuo Y (1994) Effect of the anaerobic solids retention time on enhanced biological phosphorus removal. Water Sci Technol 30:193–202Google Scholar
  118. 118.
    Goncalves RF, Rogalla F (2000) Optimising the A/O cycle for phosphorus removal in a submerged biofilter under continuous feed. Water Sci Technol 41:503–508Google Scholar
  119. 119.
    Dassanayake CY, Irvine RL (2001) An enhanced biological phosphorus removal (EBPR) control strategy for sequencing batch reactors (SBRs). Water Sci Technol 43:183–189Google Scholar
  120. 120.
    Chiou RJ, Ouyang CF, Lin KH, Chuang SH (2001) The characteristics of phosphorus removal in an anaerobic/aerobic sequential batch biofilter reactor. Water Sci Technol 44:57–65Google Scholar
  121. 121.
    Rodrigo MA, Seco A, Penyaroja JM, Ferrer J (1996) Influence of sludge age on enhanced phosphorus removal in biological systems. Water Sci Technol 34:41–48Google Scholar
  122. 122.
    Chang WC, Chiou RJ, Ouyang CF (1996) The effect of residual substrate utilization on sludge settling in an enhanced biological phosphorus removal process. Water Sci Technol 34:425–430CrossRefGoogle Scholar
  123. 123.
    Chang CH, Hao OJ (1996) Sequencing batch reactor system for nutrient removal: ORP and pH profiles. J Chem Technol Biotechnol 67:27–38CrossRefGoogle Scholar
  124. 124.
    Kargi F, Uygur A (2002) Nutrient removal performance of a sequencing batch reactor as a function of the sludge age. Enzyme Microb Technol 31:842–847CrossRefGoogle Scholar
  125. 125.
    Wang LK, Ivanov V, Tay JH, Hung YT (2010) Environmental Biotechnology. Humana Press, Totowa, NJ, pp 783–814CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Yong-Qiang Liu
    • 1
  • Yu Liu
    • 2
  • Joo-Hwa Tay
    • 3
  • Yung-Tse Hung
    • 4
  1. 1.Institute of Environmental Science and EngineeringNanyang Technological UniversitySingaporeSingapore
  2. 2.School of Civil and Environmental EngineeringNanyang Technological UniversitySingaporeSingapore
  3. 3.Department of Environmental Science and EngineeringFudan UniversityShanghaiChina
  4. 4.Department of Civil and Environmental EngineeringCleveland State UniversityClevelandUSA

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