Abstract
We describe the use of enzymatically isolated human allogeneic chondrocytes embedded in an alginate matrix in combination with a periosteal flap for the treatment of chondral and osteochondral lesions. The short-term clinical results illustrate the feasibility and safety of this procedure. This concept essentially involves a one-step surgical transplantation procedure with a well-characterized cell product in a biodegradable matrix; this is hoped to result in an easier and less time-consuming surgical procedure and in a more reproducible clinical and histological outcome than first- and second-generation autologous chondrocyte transplantation.
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References
Brittberg M, Lindahl A, Nilsson A, Ohlsson C, Isaksson O, Peterson L. Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. N Engl J Med 1994;331:889–895.
Richardson JB, Caterson B, Evans EH, Ashton BA, Roberts S. Repair of human articular cartilage after implantation of autologous chondrocytes. J Bone Joint Surg Br 1999;81: 1064–1068.
Horas U, Pelinkovic D, Herr G, Aigner T, Schnettler R. Autologous chondrocyte implantation and osteochondral cylinder transplantation in cartilage repair of the knee joint. A prospective, comparative trial. J Bone Joint Surg Am 2003;85:185–192.
Knutsen G, Engebretsen L, Ludvigsen TC, et al. Autologous chondrocyte implantation compared with microfracture in the knee. A randomized trial. J Bone Joint Surg Am 2004;86: 455–464.
Peterson L, Minas T, Brittberg M, Nilsson A, Sjogren-Jansson E, Lindahl A. Two-to 9-yr outcome after autologous chondrocyte transplantation of the knee. Clin Orthop 2000; 374:212–234.
Peterson L, Brittberg M, Kiviranta I, Akerlund EL, Lindahl A. Autologous chondrocyte transplantation. Biomechanics and long-term durability. Am J Sports Med 2002;30:2–12.
Frisbie DD, Oxford JT, Southwood L, et al. Early events in cartilage repair after subchondral bone microfracture. Clin Orthop 2003;407:215–227.
Wang L, Verbruggen G, Almqvist KF, Elewaut D, Broddelez C, Veys EM. Flow cytometric analysis of the human articular chondrocyte phenotype in vitro. Osteoarthritis Cartilage 2001;9:73–84.
Wang L, Almqvist KF, Broddelez C, Veys EM, Verbruggen G. Evaluation of chondrocyte cellassociated matrix metabolism by flow cytometry. Osteoarthritis Cartilage 2001;9:454–462.
Sohn DH, Lottman LM, Lum LY, et al. Effect of gravity on localization of chondrocytes implanted in cartilage defects. Clin Orthop 2002;394:254–262.
Zimmermann U, Mimietz S, Zimmermann H, et al. Hydrogel-based non-autologous cell and tissue therapy. Biotechniques 2000;29:564–572.
Hauselmann HJ, Fernandes RJ, Mok SS, et al. Phenotypic stability of bovine articular chondrocytes after long-term culture in alginate beads. J Cell Sci 1994; 107:17–27.
Kuettner KE, Pauli BU, Gall G, Memoli VA, Schenk RK. Synthesis of cartilage matrix by mammalian chondrocytes in vitro. I. Isolation, culture characteristics, and morphology. J Cell Biol 1982;93:743–750.
Guo JF, Jourdian GW, MacCallum DK. Culture and growth characteristics of chondrocytes encapsulated in alginate beads. Connect Tissue Res 1989; 19:277–297.
Almqvist KF, Wang L, Wang J, et al. Culture of chondrocytes in alginate surrounded by fibrin gel: characteristics of the cells over a period of eight weeks. Ann Rheum Dis 2001;60:781–790.
Almqvist KF, Wang L, Broddelez C, Veys EM, Verbruggen G. Biological freezing of human articular chondrocytes. Osteoarthritis Cartilage 2001;9:341–350.
Recht M, White LM, Winalski CS, Miniaci A, Minas T, Parker RD. MR imaging of cartilage repair procedures. Skeletal Radiol 2003;32:185–200.
Alparslan L, Winalski CS, Boutin RD, Minas T. Postoperative magnetic resonance imaging of articular cartilage repair. Semin Musculoskelet Radiol 2001;5:345–363.
Verstraete KL, Almqvist F, Verdonk P, et al. Magnetic resonance imaging of cartilage and cartilage repair. Clin Radiol 2004;59:674–689.
Verstraete K, Vandenabeele V, De Cuyper C, et al. Dynamic and delayed gadolinium-enhanced MRI of cartilage and subchondral bone after alginate allograft chondrocyte implantation (ACI). Radiology Proceedings of the 90th Scientific and Annual Meeting of the Radiological Society of North America (RSNA), 2004.
Atala A, Kim W, Paige KT, Vacanti CA, Retik AB. Endoscopic treatment of vesicoureteral reflux with a chondrocyte-alginate suspension. J Urol 1994; 152:641–643.
Genes NG, Rowley JA, Mooney DJ, Bonassar LJ. Effect of substrate mechanics on chondrocyte adhesion to modified alginate surfaces. Arch Biochem Biophys 2004;422:161–167.
Rowley JA, Madlambayan G, Mooney DJ. Alginate hydrogels as synthetic extracellular matrix materials. Biomaterials 1999;20:45–53.
Brittberg M, Sjogren-Jansson E, Thornemo M, et al. Clonal growth of human articular cartilage and the functional role of the periosteum in chondrogenesis. Osteoarthritis Cartilage 2005;13:146–153.
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© 2007 Humana Press Inc., Totowa, NJ
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Verdonk, P.C.M., Almqvist, K.F., Verdonk, R., Verstraete, K.L., Verbruggen, G. (2007). Allogeneic Chondrocyte-Based Cartilage Repair Using Alginate Beads. In: Williams, R.J. (eds) Cartilage Repair Strategies. Humana Press. https://doi.org/10.1007/978-1-59745-343-1_14
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DOI: https://doi.org/10.1007/978-1-59745-343-1_14
Publisher Name: Humana Press
Print ISBN: 978-1-58829-629-0
Online ISBN: 978-1-59745-343-1
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