Handheld and Portable Reader Devices for Lateral Flow Immunoassays

  • Konrad FaulstichEmail author
  • Roman Gruler
  • Michael Eberhard
  • Dirk Lentzsch
  • Klaus Haberstroh


In recent years, lateral flow immunoassays have become an invaluable tool for various diagnostics applications. Among the most prominent reasons for this development are their reasonable sensitivity and specificity for many applications and their rapid time-to-result readouts. The samples are applied to the test strips directly, often without the need of prior time-consuming sample preparation steps. Lateral flow immunoassays are also easy to operate and, last but not least, they do not require a device for readouts. Therefore, they are cheap and mobile. Like other technologies, however, lateral flow immunoassays also have limitations and do lack important features to further exploit this technology [1]. These shortcomings include lack of automated documentation, subjective interpretation of results leading to a high number of false positives and false negatives, lack of accurate quantitative and limited multiplexing capabilities, as well as limitation in high-throughput...


Lateral Flow Test Strip Test Line Control Band Reader Device 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We would like to thank Dr. Brendan O’Farrell of Diagnostic Consulting Network (DCN) and Dr. Bernhard Gerstenecker of Milenia Biotec for helpful discussions and evaluation of lateral flow immunoassay readers.


  1. 1.
    O’Farrell, B. and Bauer J. (2006) Developing highly sensitive, more-reproducible lateral-flow assays. Part 2: New challenges with approaches. IVD Technology 7:67.Google Scholar
  2. 2.
    Bonenberger, J. and Doumanas, M. (2006) Overcoming sensitivity limitations of lateral-flow with a novel labeling technique. IVD Technology 5:41–46.Google Scholar
  3. 3.
    Piepenburg, O., Williams, C. H., Stemple, D. L. and Armes, N. A. (2006) DNA detection using recombination proteins. PLOS Biol. 4:e204,1–7.PubMedCrossRefGoogle Scholar
  4. 4.
    Seal, J., Braven, H. and Wallace, P. (2006) Point-of-care nucleic acid lateral-flow tests. IVD Technology 8:41–54.Google Scholar
  5. 5.
    Faulstich, K., Gruler, R., Eberhard, M. and Haberstroh, K. (2007) Developing rapid mobile POC systems. Part 1: Devices and applications for lateral-flow immunodiagnostics. IVD Technology 13(6):47–53.Google Scholar
  6. 6.
    Faulstich, K., Gruler, R., Eberhard, M. and Haberstroh, K. (2007) Developing rapid mobile POC systems. Part 2: Nucleic acid based testing platforms. IVD Technology 13(7):47.Google Scholar
  7. 7.
  8. 8.
    European Patent Office Publication Number 0088636, 14.09.83, Bulletin 83/37.Google Scholar
  9. 9.
  10. 10.
  11. 11.
  12. 12.
  13. 13.
  14. 14.
  15. 15.
  16. 16.
  17. 17.
  18. 18.
  19. 19.
  20. 20.
  21. 21.
    Lenglet, L., Nikitin, P. and Pequignot, C. (2008) Magnetic immunoassays: a new paradigm in POCT. IVD Technology July/August, pp. 43–49.Google Scholar
  22. 22.
    Müller-Bardorff, M., Sylvén, C., Gundars, R., Jørgensen, B., Collinson, P. O., Waldenhofer, U., et al. (2000) Evaluation of a point-of-care system for quanti-tative determination of troponin T and myoglobin. Clin. Chem. Lab. Med. (2000) 38:567–574.CrossRefGoogle Scholar
  23. 23.
  24. 24.
  25. 25.
  26. 26.
  27. 27.
  28. 28.
    LaBorde, R. T. and O’Farrell, B. (2002) Paramagnetic-particle detection in lateral-flow assays. IVD Technology 4:36–42.Google Scholar
  29. 29.
  30. 30.

Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Konrad Faulstich
    • 1
    Email author
  • Roman Gruler
  • Michael Eberhard
  • Dirk Lentzsch
  • Klaus Haberstroh
  1. 1.ESE GmbHGermany

Personalised recommendations