Handheld and Portable Reader Devices for Lateral Flow Immunoassays
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In recent years, lateral flow immunoassays have become an invaluable tool for various diagnostics applications. Among the most prominent reasons for this development are their reasonable sensitivity and specificity for many applications and their rapid time-to-result readouts. The samples are applied to the test strips directly, often without the need of prior time-consuming sample preparation steps. Lateral flow immunoassays are also easy to operate and, last but not least, they do not require a device for readouts. Therefore, they are cheap and mobile. Like other technologies, however, lateral flow immunoassays also have limitations and do lack important features to further exploit this technology . These shortcomings include lack of automated documentation, subjective interpretation of results leading to a high number of false positives and false negatives, lack of accurate quantitative and limited multiplexing capabilities, as well as limitation in high-throughput...
KeywordsLateral Flow Test Strip Test Line Control Band Reader Device
We would like to thank Dr. Brendan O’Farrell of Diagnostic Consulting Network (DCN) and Dr. Bernhard Gerstenecker of Milenia Biotec for helpful discussions and evaluation of lateral flow immunoassay readers.
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