Manufacturing the Next Generation of Highly Sensitive and Reproducible Lateral Flow Immunoassay

  • Thomas C. TisoneEmail author
  • Brendan O’Farrell


The lateral flow immunoassay (LFIA) format that emerged in the 1980s provided the industry with a relatively standardized, open platform that could accommodate a wide range of analytes for point-of-need (PON) testing. Figure 8.1 shows a typical configuration for a LFIA strip. LFIA has been applied in application areas as diverse as human clinical diagnostics, veterinary diagnostics, plant health, environmental health and safety, food safety, and biowarfare, just to name a few [1]. To date, the application of LFIA has largely been limited to threshold or yes/no formats due to the high levels of variation exhibited with the current designs and manufacturing processes. Despite this limitation, it is estimated that over 100 different tests are currently available in the market. The ability to adapt the LFIA to a quantitative format is predicted to increase the number of marketable tests significantly.


Test Strip Roll Stock Plastic Housing Release Liner Lateral Flow Immunoassay 
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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.BioDot, Inc.Irvine

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