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Antibodies: Key to a Robust Lateral Flow Immunoassay

  • Michael C. BrownEmail author
Chapter

Introduction

Lateral flow immunoassays are well established as a valuable tool in medical, veterinary, food, agricultural, environmental, and industrial diagnostics. While in many instances they are used as a rapid screening tool and backed up by more complex and time-consuming assays, they may also be used in some applications as the primary method of choice. As a consequence, these assays need to be constructed as robustly as any other diagnostic tests. While the physical components of the lateral flow test strip, construction techniques, and buffers and surfactants play a major role in optimizing the test, at the very heart of these processes are the antibodies themselves. If the antibodies are not meticulously selected for their ability to recognize the target antigen(s) in this format, no amount of optimization will be able to overcome this inherent defect. Often, assay developers may spend great deal of time struggling to “tweak” the assay to fit their specifications, but come...

Keywords

Lateral Flow Colloidal Gold Affinity Purification Detector Particle Strong Immune Response 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Rapid Lateral Flow Test Strips, Considerations for Product Development. Bedford, MA: Millipore Corporation, (2001).Google Scholar
  2. 2.
    Nunc Guide to Solid Phase, Rowell, V. Ed., Roskilde, Denmark: Nunc A/S, Jan. 2001.Google Scholar
  3. 3.
    Chandler, J., Gurmin, T., Robinson, N. (2000) The place of gold in rapid tests. IVD Technology 6(2):37–49.Google Scholar
  4. 4.
    Linscott, D.W. Linscott’s Directory of Immunological and Biological Reagents. (2008) http://www.linscottsdirectory.com
  5. 5.
    Layton, G.T., Stanworth, D.R. and Amos, H.E. (1986) Factors influencing the immunogenicity of the haptenic drug chlorhexidine in mice. II. The role of the carrier and adjuvants in the induction of IgE and IgG anti-hapten responses. Immunology 59(3):459–465.PubMedGoogle Scholar
  6. 6.
    Harris, J.R. and Mark, J. (1999) Keyhole limpet hemocyanin (KLH): a biomedical review. Micron 30(6):597–623.PubMedCrossRefGoogle Scholar
  7. 7.
    Wong, S.S. (1991) Chemistry of Protein Conjugation and Cross-Linking. Florida: CRC Press.Google Scholar
  8. 8.
    Cuatrecasas, P. and Parikh, I. (1972) Adsorbants for affinity chromatography. Use of N-hydroxysuccinimidyl esters of agarose. J. Biol. Chem. 11(12):2291–2299.Google Scholar
  9. 9.
    Pedersen, M.K., Sorensen, N.S., Heegaard, P.M., Beyer, N.H. and Bruun, L. (2006) Effect of different hapten-carrier conjugation ratios and molecular orientations on antibody affinity against a peptide antigen. J. Immunol. Methods 311(1–2):198–206.PubMedCrossRefGoogle Scholar
  10. 10.
    Carter, J.M. (1994) Techniques for conjugation of synthetic peptides to carrier molecules. Methods Mol. Biol. 36:155–191.PubMedGoogle Scholar
  11. 11.
    Gullick, W.J. (1994) Production of antisera to synthetic peptides. Methods Mol. Biol. 32:389–399.PubMedGoogle Scholar
  12. 12.
    Walter, G. (1986) Production and use of antibodies against synthetic peptides. J. Immunol. Methods 88(2):149–161.PubMedCrossRefGoogle Scholar
  13. 13.
    Hancock, D.C. and Evan, G.I. (1998) Production and characterization of antibodies against synthetic peptides. Methods Mol. Biol. 80:15–22.PubMedCrossRefGoogle Scholar
  14. 14.
    Meloen, R.H., Puijk, W.C., Langeveld, J.P., Langedijk, J.P. and Timmerman, P. (2003) Design of synthetic peptides for diagnostics. Curr. Protein Pept. Sci. 4(4):253–260.PubMedCrossRefGoogle Scholar
  15. 15.
    Adrian, T.E. (1997) Production of antisera using peptide conjugates. Methods Mol. Biol. 73:239–249.PubMedGoogle Scholar
  16. 16.
    Chambers, R.S. and Johnston, S.A. (2003) High-level generation of polyclonal antibodies by genetic immunization. Nat. Biotechnol. 21(9):1088–1092.PubMedCrossRefGoogle Scholar
  17. 17.
    Tang, D.C., DeVit, M. and Johnston, S.A. (1992) Genetic immunization is a simple method for eliciting an immune response. Nature 356:152–154.PubMedCrossRefGoogle Scholar
  18. 18.
    Thalhamer, J., Leitner, W., Hammer, P. and Brtko, J. (2001) Designing immune responses with genetic immunization and immunostimulatory DNA sequences. Endocr. Regul. 35(3):143–166.PubMedGoogle Scholar
  19. 19.
    Cohen, A.D., Boyer, J.D. and Weiner, D.B. (1998) Modulating the immune response to genetic immunization. FASEB J. 12(15):1611–1626.PubMedGoogle Scholar
  20. 20.
    Bailey, G.S. (1994) The raising of a polyclonal antiserum to a protein. Methods Mol. Biol. 32:381–388.PubMedGoogle Scholar
  21. 21.
    Hurn, B.A. and Chantler, S.M. (1980) Production of reagent antibodies. Methods Enzymol. 70(A):104–142.PubMedCrossRefGoogle Scholar
  22. 22.
    Drenckhahn, D., Jons, T. and Schmitz, F. (1993) Production of polyclonal antibodies against proteins and peptides. Methods Cell Biol. 37:7–56.PubMedCrossRefGoogle Scholar
  23. 23.
    Harlow, E. and Lane, D. (1988) Using Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.Google Scholar
  24. 24.
    Johnson, A.G. (1994) Molecular adjuvants and immunomodulators: new approaches to immunization. Clin Microbiol. Rev. 7(3):277–289.PubMedGoogle Scholar
  25. 25.
    Stills, H.F., Jr. (2005) Adjuvants and antibody production: dispelling the myths associated with Freund’s complete and other adjuvants. ILAR J. 46(3):280–293.PubMedGoogle Scholar
  26. 26.
    Leenaars, M. and Hendriksen, C.F. (2005) Critical steps in the production of polyclonal and monoclonal antibodies: evaluation and recommendations. ILAR J. 46(3):269–279.PubMedGoogle Scholar
  27. 27.
    Schunk, M.K. and Macallum, G.E. (2005) Applications and optimization of immunization procedures. ILAR J. 46(3):241–257.PubMedGoogle Scholar
  28. 28.
    Tini, M., Jewell, U.R., Camenisch, G., Chilov, D. and Gassmann, M. (2002) Generation and application of chicken egg-yolk antibodies. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 131(3):569–574.PubMedCrossRefGoogle Scholar
  29. 29.
    Carlander, D., Stalberg, J. and Larsson, A. (1999) Chicken antibodies: a clinical chemistry perspective. Ups. J. Med. Sci. 104(3):179–189.PubMedCrossRefGoogle Scholar
  30. 30.
    Saito, M., Sakurai, S., Motegi, A., Saito, K., Sano, T. and Nakajima, T. (2007) Comparative study using rabbit-derived polyclonal, mouse-derived monoclonal, and rabbit-derived monoclonal antibodies for KIT immunostaining in GIST and other tumors. Pathol. Int. 57(4):200–204.PubMedCrossRefGoogle Scholar
  31. 31.
    Fisher, P.A. and Smith, D.E. (1988) Affinity purification of antibodies using antigens immobilized on solid supports. Biochem. Soc. Trans. 16(2):134–138.PubMedGoogle Scholar
  32. 32.
    Hermanson, G.T., Mallia, A.K. and Smith, P.K. (1992) Immobilized Affinity Ligand Technique. California: Academic Press.Google Scholar
  33. 33.
    Dean, P.D.G., Johnson, W. S. and Middle, F.A. (1985) Affinity Chromatography: A Practical Approach. Oxford: IRI Press.Google Scholar
  34. 34.
    Jack, G.W. (1994) related articles, immunoaffinity chromatography. Mol. Biotechnol. 1(1):59–86.PubMedCrossRefGoogle Scholar
  35. 35.
    Fornstedt, N. (1984) Affinity chromatographic studies on antigen-antibody dissociation. FEBS Lett. 177(2):195–199.PubMedCrossRefGoogle Scholar
  36. 36.
    Hoffken, K., Bosse, F., Steih, U. and Schmidt, C.G. (1982) Dissociation and isolation of antigen and antibody from immune complexes. J. Immunol. Methods 53(1):51–59.PubMedCrossRefGoogle Scholar
  37. 37.
    Tsang, V.C. and Wilkins, P.P. (1991) Optimum dissociating condition for immunoaffinity and preferential isolation of antibodies with high specific activity. J. Immunol. Methods 138(2):291–299.PubMedCrossRefGoogle Scholar
  38. 38.
    Kummer, A. and Li-Chan, E.C. (1998) Application of an ELISA-elution assay as a screening tool for dissociation of yolk antibody-antigen complexes. J. Immunol. Methods 211(1–2):125–137.PubMedCrossRefGoogle Scholar
  39. 39.
    De Roe, C., Courtroy, P.J. and Baudhuin, P. (1987) A model of protein-colloidal gold interactions. J. Histochem. Cytochem. 35(11):1191–1198.PubMedCrossRefGoogle Scholar
  40. 40.
    Horisberger, M., Rosset, J. and Bauer, H. (1975) Colloidal gold granules as markers for cell surface receptors in the scanning electron microscope. Experimentia 31:1147–1149.CrossRefGoogle Scholar
  41. 41.
    De May, J. (1986) Colloidal gold probes. In: Pollack, J. and van Noorden, S., Eds. Immunochemistry, Modern Methods and Applications, Bristol: Wright PSG press, pp. 82–92.Google Scholar

Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Strategic DiagnosticsNewark

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