Antibodies: Key to a Robust Lateral Flow Immunoassay

  • Michael C. BrownEmail author


Lateral flow immunoassays are well established as a valuable tool in medical, veterinary, food, agricultural, environmental, and industrial diagnostics. While in many instances they are used as a rapid screening tool and backed up by more complex and time-consuming assays, they may also be used in some applications as the primary method of choice. As a consequence, these assays need to be constructed as robustly as any other diagnostic tests. While the physical components of the lateral flow test strip, construction techniques, and buffers and surfactants play a major role in optimizing the test, at the very heart of these processes are the antibodies themselves. If the antibodies are not meticulously selected for their ability to recognize the target antigen(s) in this format, no amount of optimization will be able to overcome this inherent defect. Often, assay developers may spend great deal of time struggling to “tweak” the assay to fit their specifications, but come...


Lateral Flow Colloidal Gold Affinity Purification Detector Particle Strong Immune Response 
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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Strategic DiagnosticsNewark

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