Quantitative, False Positive, and False Negative Issues for Lateral Flow Immunoassays as Exemplified by Onsite Drug Screens

  • Raphael C. WongEmail author
  • Harley Y. Tse


Lateral flow immunoassay devices offer many advantages including convenience, economical, simplicity, and rapid result. Many lateral flow immunoassays are non-instrumental and rely on visual detection of colored lines for results, enabling easy portability and allowing testing at any time and at any place by non-technical personnel. Hence, many lateral flow immunoassay tests have been developed for use “onsite”, “point-of-care”, or “point-of-test”. However, utilization of this type of device requires the acceptance of some trade-offs. The most important is that the test results are generally qualitative. Moreover, since it is antibody-based, possibility exists that chemicals with similar structures will cause positive result leading to specificity and sensitivity issues. Recognizing such limitations, this technology has been widely utilized for screening tests in which a yes and no answer is sufficient. The initial screen test result can then be confirmed by a quantitative...


Test Line Drug Screen False Result Poppy Seed Liquid Front 
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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Branan Medical CorporationIrvineUSA

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