Abstract
Candida rugosa lipase was entrapped in silica sol-gel particles prepared by hydrolysis of methyltrimethoxysilane and assayed by p-nitrophenyl palmi-tate hydrolysis, as a function of pH and temperature, giving pH optima of 7.8 (free enzyme) and 5.0–8.0 (immobilized enzyme). The optimum temperature for the immobilized enzyme (50–55°C) was 19°C higher than for the free enzyme. Thermal, operational, and storage stability were determined with n-butanol and butyric acid, giving at 45°C a half-life 2.7 times greater for the immobilized enzyme; storage time was 21 d at room temperature. For ester synthesis, the optimum temperature was 47°C, and high esterification conversions were obtained under repeated batch cycles (half-life of 138 h).
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References
Hwang, S., Lee, K. T., Park, J. W., Min, B. R., Haam, S., Ahn, I. S., and Jung, J. K. (2004), Biochem. Eng. J. 17, 85–90.
Pierre, A. and Buisson, P. (2001), J. Mol. Catal. B. Enzymat. 11, 639–647.
Reetz, M. T. (1997), Adv. Mater. 12, 943–954.
Fu, X., Zhu, X., Gao, K., and Duan, J. (1995), J. A. Oil Chem. Soc. 72(5), 527–533.
Villeneuve, P., Muderhwa, J. M, Graille, J. M., and Haas, M. J. (2000), J. Mol. Catal. B: Enzymat. 9(4–6), 113–148.
Tisher, W. and Kasche, V. (1999), TIBTECH 17, 223–232.
Bagi, K., Simon, M. and Szaja, B. (1997), Enzyme Microb. Technol. 20, 531–535.
Oliveira, P. C, Alves, G. M., and Castro, H. F. (2000), Biochem. Eng. J. 5, 63–71.
Buisson, P., Hernandez, C., Pierre, M., and Pierre, A. C. (2001), J. Non-Crystalline Solids 285, 295–302.
Reetz, M. T., Zonta, A., and Simpelkamp, J. (1996), Biotechnol. Bioeng. 49, 527–534.
Kawakami, K., Matsui, Y, Ono, T. and Hiroyuki, H. (2003), Biocatal. Biotransform. 21, 49–52.
Alfaya, A. and Kubota, L. T. (2002), Quint. Nova 25, 935–841.
Kauffmann, C. and Mandelbaum, R. T. (1998), J. Biotechnol. 62, 169–176.
Fernandez-Lafuente, R., Sabuquilho, P., Fernandez-Lorente, G., and Gguisán, J. M. (1998), Chem. Phys. Lipids 93, 185–197.
Faber, K. (1997), in Biotransformations in Organic Chemistry: A Textbook, Springer-Verlag, Berlin, pp. 21.
Lima, A. W. O. and Angnes, L. (1999), Quint. Nova 22(2), 229–245.
Reslow, M., Adlercreutz, P., and Mattiason, B. (1988), Eur. J. Biochem. 172, 573–578.
Soares, C. M. F., Santos, O. A. S., de Castro, H. E, de Moraes, F. E, and Zanin, G. M. (2004), Appl. Biochem. Biotechnol. 113–116, 307–319.
Soares, C. M. E, Santos, O. A. S., Olivo, J. E., de Castro, H. E, de Moraes, E E, and Zanin, G. M. (2004), J. Mol. Catal. B: Enzymat. 29, 69–79.
Iza, M., Worley, S., Danumah, C, Kaliagine, S., and Bousmina, M. (2000), Polymer 41, 5885–5893.
Kordel, M., Hofmann, B., Schomburg, D., and Schmid, R. D. (1991), J. Bacteriol. 173(15), 4836–4841.
Pereira, E. B., Castro, H. E, Moraes, E E, and Zanin, G. M. (2001) Appl. Biochem. Biotechnol. 91–93, 739–752.
Cabral, J. M. S., Best, D., Boross, L., and Tramper, J. (1994), Applied Biocatalysis., Harwood Academic Publishers, Chur.
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Soares, C.M.F., de Castro, H.F., Itako, J.E., de Moraes, F.F., Zanin, G.M. (2005). Characterization of Sol-Gel Bioencapsulates for Ester Hydrolysis and Synthesis. In: Davison, B.H., Evans, B.R., Finkelstein, M., McMillan, J.D. (eds) Twenty-Sixth Symposium on Biotechnology for Fuels and Chemicals. ABAB Symposium. Humana Press. https://doi.org/10.1007/978-1-59259-991-2_72
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DOI: https://doi.org/10.1007/978-1-59259-991-2_72
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