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Continuous Hydrogen Photoproduction by Chlamydomonas reinhardtii

Using a Novel Two-Stage, SuIfate-Limited Chemostat System

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Twenty-Sixth Symposium on Biotechnology for Fuels and Chemicals

Part of the book series: ABAB Symposium ((ABAB))

Abstract

This study demonstrates, for the first time, that it is possible to couple sulfate-limited Chlamydomonas reinhardtii growth to continuous H2 photoproduction for more than 4000 h. A two-stage chemostat system physically separates photosynthetic growth from H2 production, and it incorporates two automated photobioreactors (PhBRs). In the first PhBR, the algal cultures are grown aerobically in chemostat mode under limited sulfate to obtain photosynthetically competent cells. Active cells are then continuously delivered to the second PhBR, where H2 production occurs under anaerobic conditions. The dependence of the H2 production rate on sulfate concentration in the medium, dilution rates in the PhBRs, and incident light intensity is reported.

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Fedorov, A.S., Kosourov, S., Ghirardi, M.L., Seibert, M. (2005). Continuous Hydrogen Photoproduction by Chlamydomonas reinhardtii . In: Davison, B.H., Evans, B.R., Finkelstein, M., McMillan, J.D. (eds) Twenty-Sixth Symposium on Biotechnology for Fuels and Chemicals. ABAB Symposium. Humana Press. https://doi.org/10.1007/978-1-59259-991-2_35

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