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Total, Complexed, and Free PSA Forms and Human Glandular Kallikrein 2

Clinical Application for Early Detection and Staging of Prostate Cancer
  • Alexander Haese
  • Alan W. Partin
Chapter
  • 137 Downloads
Part of the Current Clinical Urology book series (CCU)

Abstract

In the United States, extensive use of prostate-specific antigen (PSA) for early detection of prostate cancer prostate cancer was responsible for a steady increase in the incidence of clinically and pathologically localized prostate cancer for more than a decade, with the incidence of locally advanced or metastatic disease steadily declining (1). The massive impact of PSA on the presentation of prostate cancer has caused concerns of overdetection and initiation of unnecessary treatment for so-called clinically insignificant prostate cancer. Recently, a decrease in prostate cancer incidence was noted, and today the incidence is only minimally higher than that seen in the pre-PSA era (2), suggesting that PSA is effective as a screening tool. An effective screening tool will increase the detection of a certain disease; however, the incidence should decrease over time, if significant disease is detected through that screening method. If, however the incidence does not decline, it is possible that insignificant disease may be detected.

Keywords

Prostate Cancer Benign Prostatic Hyperplasia Radical Prostatectomy Localize Prostate Cancer Prostate Cancer Detection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Mettlin CJ, Murphy GP, Ho R, et al. The National Cancer Data Base report on longitudinal observations on prostate cancer. Cancer 1996; 77: 2162.Google Scholar
  2. 2.
    Wingo PA, Landis S, Ries LA. An adjustment to the 1997 estimate for new prostate cancer cases. Cancer 1997; 80: 1810.PubMedCrossRefGoogle Scholar
  3. 3.
    Ito K, Kubota Y, Suzuki K, et al. Correlation of prostate-specific antigen before prostate cancer detection and clinicopathologic features: evaluation of mass screening populations. Urology 2000; 55: 705.PubMedCrossRefGoogle Scholar
  4. 4.
    Plawker MW, Fleisher JM, Vapnek EM, et al. Current trends in prostate cancer diagnosis and staging among United States urologists. J Urol 1997; 158: 1853.PubMedCrossRefGoogle Scholar
  5. 5.
    Partin AW, Kattan MW, Subong EN, et al. Combination of prostate-specific antigen, clinical stage, and Gleason score to predict pathological stage of localized prostate cancer. A multi-institutional update. Jama 1997; 277: 1445.PubMedCrossRefGoogle Scholar
  6. 6.
    Partin AW, Mangold LA, Lamm DM, Walsh PC, Epstein JI, Pearson JD. Contemporary update of prostate cancer staging nomograms (Partin tables) for the new millenium. Urology 2001; 6: 843–848.CrossRefGoogle Scholar
  7. 7.
    Gann PH, Hennekens CH, Stampfer MJ. A prospective evaluation of plasma prostate-specific antigen for detection of prostatic cancer. JAMA 1995; 273: 289.PubMedCrossRefGoogle Scholar
  8. 8.
    Stamey TA, Freiha FS, McNeal JE, et al. Localized prostate cancer. Relationship of tumor volume to clinical significance for treatment of prostate cancer. Cancer 1993; 71: 933.PubMedCrossRefGoogle Scholar
  9. 9.
    Epstein JI, Chan DW, Sokoll LJ, et al. Nonpalpable stage T1c prostate cancer: prediction of insignificant disease using free/total prostate specific antigen levels and needle biopsy findings. J Urol 1998; 160: 2407.Google Scholar
  10. 10.
    Belville WD. Are T1c tumors different from incidental tumors found at autopsy? The risk and reality of overdetection. Semin Urol Oncol 1995; 13: 181.PubMedGoogle Scholar
  11. 11.
    Mettlin C, Murphy GP, Lee F, et al. Characteristics of prostate cancer detected in the American Cancer Society-National Prostate Cancer Detection Project. J Urol 1994; 152: 1737.PubMedGoogle Scholar
  12. 12.
    Catalona WJ, Smith DS, Ratliff TL, et al. Detection of organ-confined prostate cancer is increased through prostate-specific antigen-based screening. JAMA 1993; 270: 948.PubMedCrossRefGoogle Scholar
  13. 13.
    Herschman JD, Smith DS, Catalona WJ. Effect of ejaculation on serum total and free prostate-specific antigen concentrations. Urology 1997; 50: 239.PubMedCrossRefGoogle Scholar
  14. 14.
    Oesterling JE, Rice DC, Glenski WJ, et al. Effect of cystoscopy, prostate biopsy, and transurethral resection of prostate on serum prostate-specific antigen concentration. Urology 1993; 42: 276.PubMedCrossRefGoogle Scholar
  15. 15.
    Pannek J, Marks LS, Pearson JD, et al. Influence of finasteride on free and total serum prostate specific antigen levels in men with benign prostatic hyperplasia. J Urol 1998; 159: 449.PubMedCrossRefGoogle Scholar
  16. 16.
    Catalona WJ, Smith DS, Ornstein DK. Prostate cancer detection in men with serum PSA concentrations of 2.6 to 4.0 ng/mL and benign prostate examination. Enhancement of specificity with free PSA measurements. JAMA 1997; 277: 1452.PubMedCrossRefGoogle Scholar
  17. 17.
    Lodding P, Aus G, Bergdahl S, et al. Characteristics of screening detected prostate cancer in men 50 to 66 years old with 3 to 4 ng./ml. Prostate specific antigen. J Urol 1998; 159: 899.PubMedCrossRefGoogle Scholar
  18. 18.
    Catalona WJ, Richie JP, Ahmann FR, et al. Comparison of digital rectal examination and serum prostate specific antigen in the early detection of prostate cancer: results of a multicenter clinical trial of 6,630 men. J Urol 1994; 151: 1283.PubMedGoogle Scholar
  19. 19.
    Cooner WH, Mosley BR, Rutherford CL, Jr, et al. Prostate cancer detection in a clinical urological practice by ultrasonography, digital rectal examination and prostate specific antigen. J Urol 1990; 143: 1146.PubMedGoogle Scholar
  20. 20.
    Hammerer P, Huland H. Systematic sextant biopsies in 651 patients referred for prostate evaluation. J Urol 1994; 151: 99.PubMedGoogle Scholar
  21. 21.
    Ellis WJ, Chetner MP, Preston SD, et al. Diagnosis of prostatic carcinoma: the yield of serum prostate specific antigen, digital rectal examination and transrectal ultrasonography. J Urol 1994; 152: 1520.PubMedGoogle Scholar
  22. 22.
    Lilja H, Christensson A, Dahlen U, et al. Prostate-specific antigen in serum occurs predominantly in complex with alpha 1-antichymotrypsin. Clin Chem 1991; 37: 1618.PubMedGoogle Scholar
  23. 23.
    Christensson A, Laurell CB, Lilja H. Enzymatic activity of prostate-specific antigen and its reactions with extracellular serine proteinase inhibitors. Eur J Biochem 1990; 194: 755.PubMedCrossRefGoogle Scholar
  24. 24.
    Christensson A, Bjork T, Nilsson O, et al. Serum prostate specific antigen complexed to alpha 1- antichymotrypsin as an indicator of prostate cancer. J Urol 1993; 150: 100.PubMedGoogle Scholar
  25. 25.
    Stenman UH, Leinonen J, Alfthan H, et al. A complex between prostate-specific antigen and alpha 1- antichymotrypsin is the major form of prostate-specific antigen in serum of patients with prostatic cancer: assay of the complex improves clinical sensitivity for cancer. Cancer Res 1991; 51: 222.PubMedGoogle Scholar
  26. 26.
    Luderer AA, Chen YT, Soriano TF, et al. Measurement of the proportion of free to total prostate-specific antigen improves diagnostic performance of prostate-specific antigen in the diagnostic gray zone of total prostate-specific antigen. Urology 1995; 46: 187.PubMedCrossRefGoogle Scholar
  27. 27.
    Prestigiacomo AF, Lilja H, Pettersson K, et al. A comparison of the free fraction of serum prostate specific antigen in men with benign and cancerous prostates: the best case scenario. J Urol 1996; 156: 350.PubMedCrossRefGoogle Scholar
  28. 28.
    Catalona WJ, Partin AW, Slawin KM, et al. Use of the percentage of free prostate-specific antigen to enhance differentiation of prostate cancer from benign prostatic disease: a prospective multicenter clinical trial. JAMA 1998; 279: 1542.PubMedCrossRefGoogle Scholar
  29. 29.
    Carter HB, Partin AW, Luderer AA, et al. Percentage of free prostate-specific antigen in sera predicts aggressiveness of prostate cancer a decade before diagnosis. Urology 1997; 49: 379.PubMedCrossRefGoogle Scholar
  30. 30.
    Semjonow A, De Angelis G, Schmidt HP. Variability of immunoassays for PSA, in Prostate-Specific Antigen, vol 1. Edited by M. K. Brawer. Dekker, New York, 2001.Google Scholar
  31. 31.
    Lilja H, Haese A, Bjork T, et al. Significance and metabolism of complexed and noncomplexed prostate specific antigen forms, and human glandular kallikrein 2 in clinically localized prostate cancer before and after radical prostatectomy. J Urol 1999; 162: 2029.Google Scholar
  32. 32.
    Haese A, Graefen M, Noldus J, et al. Prostatic volume and ratio of free-to-total prostate specific antigen in patients with prostatic cancer or benign prostatic hyperplasia. J Urol 1997; 158: 2188.Google Scholar
  33. 33.
    Partin AW, Catalona WJ, Southwick PC, et al. Analysis of percent free prostate-specific antigen (PSA) for prostate cancer detection: influence of total PSA, prostate volume, and age. Urology 1996; 48: 55.PubMedCrossRefGoogle Scholar
  34. 34.
    Bjork T, Piironen T, Pettersson K, et al. Comparison of analysis of the different prostate-specific antigen forms in serum for detection of clinically localized prostate cancer. Urology 1996; 48: 882.PubMedCrossRefGoogle Scholar
  35. 35.
    Christensson A, Lilja H. Complex formation between protein C inhibitor and prostate-specific antigen in vitro and in human semen. Eur J Biochem 1994; 220: 45.PubMedCrossRefGoogle Scholar
  36. 36.
    Leinonen J, Zhang WM, Paus E, et al. Reactivity of 77 antibodies to prostate-specific antigen with isoenzymes and complexes of prostate-specific antigen. Tumour Biol 1999; 20 (suppl 1): 28.PubMedCrossRefGoogle Scholar
  37. 37.
    Pettersson K, Piironen T, Seppala M, et al. Free and complexed prostate-specific antigen (PSA): in vitro stability, epitope map, and development of immunofluorometric assays for specific and sensitive detection of free PSA and PSA-alpha 1-antichymotrypsin complex. Clin Chem 1995; 41: 1480.PubMedGoogle Scholar
  38. 38.
    Allard WJ, Zhou Z, Yeung KK. Novel immunoassay for the measurement of complexed prostate-specific antigen in serum. Clin Chem 1998; 44: 1216.PubMedGoogle Scholar
  39. 39.
    Bjork T, Ljungberg B, Piironen T, et al. Rapid exponential elimination of free prostate-specific antigen contrasts the slow, capacity-limited elimination of PSA complexed to alpha 1-antichymotrypsin from serum. Urology 1998; 51: 57.PubMedCrossRefGoogle Scholar
  40. 40.
    Piironen T, Pettersson K, Suonpaa M, et al. In vitro stability of free prostate-specific antigen (PSA) and prostate-specific antigen (PSA) complexed to alpha 1-antichymotrypsin in blood samples. Urology 1996; 48: 81.PubMedCrossRefGoogle Scholar
  41. 41.
    Woodrum D, French C, Shamel LB. Stability of free prostate-specific antigen in serum samples under a variety of sample collection and sample storage conditions. Urology 1996; 48: 33.PubMedCrossRefGoogle Scholar
  42. 42.
    Okihara K, Fritsche H, Ayala A, et al. Can complexed prostate-specific antigen enhance prostate cancer detection in men with total prostate specific antigen between 2.4 and 4 ng/ml? J Urol 2001; 165: 1930.PubMedCrossRefGoogle Scholar
  43. 43.
    Mitchell IDC, Croal BL, Dickie A, et al. A. prospective study to evaluate the role of complexed prostate-specific antigen and free/total prostate-specific antigen ratio for the diagnosis of prostate cancer. J Urol 2001; 165: 1549.PubMedCrossRefGoogle Scholar
  44. 44.
    Brawer MK, Cheli CD, Neaman IE, et al. Complexed prostate specific antigen provides significant enhancement of specificity compared with total prostate specific antigen for detecting prostate cancer. J Urol 2000; 163: 1476.PubMedCrossRefGoogle Scholar
  45. 45.
    Okegawa T, Noda H, Nutahara K, et al. Comparison of two investigative assays for the complexed prostate-specific antigen in total prostate-specific antigen between 4.1 and 10ng/ml. Urology 2000;55:700–704, 2000; 55: 700.Google Scholar
  46. 46.
    Mikolajczyk SD, Millar LS, Wang TJ, et al. “BPSA”, a specific molecular form of free prostate-specific antigen is found predominantly in the transition zone of patients with nodular benign prostatic hyperplasia. Urology 2000; 55: 41.PubMedCrossRefGoogle Scholar
  47. 47.
    Mikolajczyk SD, Rittenhouse HG. BPSA and pPSA are complementary forms of PSA that are found, respectively, in the serum of men with benign and malignant prostate disease. J Clin Lig Assay, submitted, 2001.Google Scholar
  48. 48.
    Mikolajczyk SD, Millar LS, Wang TJ, et al. A precursor form of prostate-specific antigen is more highly elevated in prostate cancer compared with benign transition zone prostate tissue. Cancer Res 2000; 60: 756.PubMedGoogle Scholar
  49. 49.
    Nurmikko P, Vaisanen V, Piironen T, et al. Production and characterisation of novel anti-prostate-specific antigen (PSA) monoclonal antibodies that do not detect internally cleaved Lys145-Lys146 inactive PSA. Clin Chem 2000; 46: 1610.PubMedGoogle Scholar
  50. 50.
    Steuber T, Haese A, Becker C, et al. Determination of a cancer-specific form of free PSA—intact PSA—discriminates by statistical significance patients with a negative systematic prostate biopsy from those with biopsy proven prostate cancer. J Urol 2001;165(suppl):1162 A.Google Scholar
  51. 51.
    Benson MC, Whang IS, Olsson CA, et al. The use of prostate specific antigen density to enhance the predictive value of intermediate levels of serum prostate specific antigen. J Urol 1992; 147: 817.PubMedGoogle Scholar
  52. 52.
    Partin AW, Carter HB, Chan DW, et al. Prostate specific antigen in the staging of localized prostate cancer: influence of tumor differentiation, tumor volume and benign hyperplasia. J Urol 1990; 143: 747.PubMedGoogle Scholar
  53. 53.
    Stamey TA, Kabalin JN, McNeal JE, et al. Prostate specific antigen in the diagnosis and treatment of adenocarcinoma of the prostate. II. Radical prostatectomy treated patients. J Urol 1989; 141: 1076.PubMedGoogle Scholar
  54. 54.
    Seaman E, Whang IS, Olsson CA, et al. PSA-density (PSAD). Role in patient evaluation and management. Urol Clin North Am 1993; 20: 635.Google Scholar
  55. 55.
    Catalona WJ, Richie JP, de Kernion JB, et al. Comparison of prostate-specific antigen concentration versus prostate-specific antigen density in the early detection of prostate cancer: receiver operator characteristic curves. J Urol 1994; 152: 2031.Google Scholar
  56. 56.
    Brawer MK, Aramburu EAG, Chen GL, et al. The inability of prostate-specific antigen index to enhance the predictive value of prostate-specific antigen in the diagnosis of prostatic carcinoma. J Urol 1993; 150: 369.PubMedGoogle Scholar
  57. 57.
    Djavan B, Zlotta AR, Byttebier G, et al. Prostate specific antigen density of the transition zone for early detection of prostate cancer. J Urol 1998; 160: 411.PubMedCrossRefGoogle Scholar
  58. 58.
    Djavan B, Marberger M, Zlotta AR, et al. PSA, f/tPSA, PSAD, PSA-TZ and PAS-velocity for prostate cancer prediction: a multivariate analysis. J Urol 1998; 159: 898 (A).Google Scholar
  59. 59.
    Carter HB, Pearson JD. PSA velocity for the diagnosis of early prostate cancer. A new concept. Urol Clin North Am 1993; 20: 665.PubMedGoogle Scholar
  60. 60.
    Nadler RB, Humphrey PA, Smith DS, et al. Effect of inflammation and benign prostatic hyperplasia on elevated serum prostate specific antigen levels. J Urol 1995; 154: 407.PubMedCrossRefGoogle Scholar
  61. 61.
    Nixon RG, Wener MH, Smith KM. Biolgical variation of prostate specific antigen levels in serum: an evaluation of day-to-day physiological fluctuations in a well-defined cohort of 24 patients. J Urol 1997; 157: 2183.Google Scholar
  62. 62.
    Hoekx L, Jeuris W, Van Marck E, et al. Elevated serum prostate specific antigen (PSA) related to asymptomatic prostatic inflammation. Acta Urol Belg 1998; 66: 1.PubMedGoogle Scholar
  63. 63.
    Oesterling JE. Age-specific reference ranges for serum PSA. N Engl J Med 1996; 335: 345.PubMedCrossRefGoogle Scholar
  64. 64.
    Partin AW, Criley SR, Subong EN, et al. Standard versus age-specific prostate-specific antigen reference ranges among men with clinically localized prostate cancer: a pathological analysis. J Urol 1996; 155: 1336.PubMedCrossRefGoogle Scholar
  65. 65.
    Reissigl A, Pointner J, Horniger W, et al. Comparison of different prostate-specific antigen cutpoints for early detection of prostate cancer: results of a large screening study. Urology 1995; 46: 662.PubMedCrossRefGoogle Scholar
  66. 66.
    Littrup PJ, Kane RA, Mettlin C, et al. Cost-effective prostate cancer detection. Reduction of low-yield biopsies. Cancer 1994; 74: 3146.PubMedCrossRefGoogle Scholar
  67. 67.
    Morgan TO, Jacobsen SJ, McCarthy WF, et al. Age-specific reference ranges for prostate-specific antigen in black men. N Engl J Med 1996; 335: 304.PubMedCrossRefGoogle Scholar
  68. 68.
    Oesterling JE, Kumamoto Y, Tsukamoto T, et al. Serum prostate-specific antigen in a community based population of health Japanese men: lower values than for similarly aged white men. Br J Urol 1995; 75: 347.PubMedCrossRefGoogle Scholar
  69. 69.
    Schedlich LJ, Bennetts BH, Morris BJ. Primary structure of a human glandular kallikrein gene. DNA 1987; 6: 429.PubMedCrossRefGoogle Scholar
  70. 70.
    Young CY, Andrews PE, Montgomery BT, et al. Tissue-specific and hormonal regulation of human prostate-specific glandular kallikrein. Biochemistry 1992; 31: 818.PubMedCrossRefGoogle Scholar
  71. 71.
    Darson MF, Pacelli A, Roche P, et al. Human glandular kallikrein 2 (hK2) expression in prostatic intraepithelial neoplasia and adenocarcinoma: a novel prostate cancer marker. Urology 1997; 49: 857.PubMedCrossRefGoogle Scholar
  72. 72.
    Darson MF, Pacelli A, Roche P, et al. Human glandular kallikrein 2 expression in prostate adenocarcinoma and lymph node metastases. Urology 1999; 53: 939.PubMedCrossRefGoogle Scholar
  73. 73.
    Siivola P, Pettersson K, Piironen T, et al. Time-resolved fluorescence imaging for specific and quantitative immunodetection of human kallikrein 2 and prostate-specific antigen in prostatic tissue sections. Urology 2000; 56: 682.PubMedCrossRefGoogle Scholar
  74. 74.
    Piironen T, Lovgren J, Karp M, et al. Immunofluorometric assay for sensitive and specific measurement of human prostatic glandular kallikrein (hK2) in serum. Clin Chem 1996; 42: 1034.PubMedGoogle Scholar
  75. 75.
    Becker C, Piironen T, Kiviniemi J, et al. Sensitive and specific immunodetection of human glandular kallikrein 2 in serum. Clin Chem 2000; 46: 198.PubMedGoogle Scholar
  76. 76.
    Klee GG, Goodmanson MK, Jacobsen SJ, et al. Highly sensitive automated chemiluminometric assay for measuring free human glandular kallikrein-2. Clin Chem 1999; 45: 800.PubMedGoogle Scholar
  77. 77.
    Becker C, Piironen T, Pettersson K, et al. Discrimination of men with prostate cancer from those with benign disease by measurements of human glandular kallikrein 2 (HK2) in serum. J Urol 2000; 163: 311.PubMedCrossRefGoogle Scholar
  78. 78.
    Partin AW, Catalona WJ, Finlay JA, et al. Use of human glandular kallikrein 2 for the detection of prostate cancer: preliminary analysis. Urology 1999; 54: 839.PubMedCrossRefGoogle Scholar
  79. 79.
    Grauer LS, Finlay JA, Mikolajczyk SD, et al. Detection of human glandular kallikrein, hK2, as its precursor form and in complex with protease inhibitors in prostate carcinoma serum. J Androl 1998; 19: 407.PubMedGoogle Scholar
  80. 80.
    Saedi MS, Hill TM, Kuus-Reichel K, et al. The precursor form of the human kallikrein 2, a kallikrein homologous to prostate-specific antigen, is present in human sera and is increased in prostate cancer and benign prostatic hyperplasia. Clin Chem 1998; 44: 2115.Google Scholar
  81. 81.
    Nam RK, Diamandis EP, Toi A, et al. Serum human glandular kallikrein-2 protease levels predict the presence of prostate cancer among men with elevated prostate-specific antigen. J Clin Oncol 2000; 18: 1036.PubMedGoogle Scholar
  82. 82.
    Klee GG, Young CY, Tindall DJ. Human glandular kallikrein protein, in Prostate-Specific Antigen, vol 1. Edited by M. K. Brawer. Dekker, New York, 2001, pp. 283–296.Google Scholar
  83. 83.
    Magklara A, Scorilas A, Catalona WJ, et al. The combination of human glandular kallikrein and free prostate-specific antigen (PSA) enhances discrimination between prostate cancer and benign prostatic hyperplasia in patients with moderately increased total PSA. Clin Chem 1999; 45: 1960.PubMedGoogle Scholar
  84. 84.
    Becker C, Piironen T, Pettersson K, et al. Clinical value of human glandular kallikrein 2 and free and total prostate-specific antigen in serum from a population of men with prostate-specific antigen levels 3.0 ng/mL or greater. Urology 2000; 55: 694.PubMedCrossRefGoogle Scholar
  85. 85.
    Kwiatkowski MK, Recker F, Piironen T, et al. In prostatism patients the ratio of human glandular kallikrein to free PSA improves the discrimination between prostate cancer and benign hyperplasia within the diagnostic “gray zone” of total PSA 4 to 10 ng/mL. Urology 1998; 52: 360.PubMedCrossRefGoogle Scholar
  86. 86.
    Epstein JI, Pizov G, Walsh PC. Correlation of pathologic findings with progression after radical retropubic prostatectomy. Cancer 1993; 71: 3582.PubMedCrossRefGoogle Scholar
  87. 87.
    Pound CR, Partin AW, Epstein JI, et al. Prostate-specific antigen after anatomic radical retropubic prostatectomy. Patterns of recurrence and cancer control. Urol Clin North Am 1997; 24: 395.PubMedCrossRefGoogle Scholar
  88. 88.
    McNeal JE, Villers AA, Redwine EA, et al. Capsular penetration in prostate cancer. Significance for natural history and treatment. Am J Surg Pathol 1990; 14: 240.PubMedCrossRefGoogle Scholar
  89. 89.
    Oesterling JE, Chan DW, Epstein JI, et al. Prostate specific antigen in the preoperative and postoperative evaluation of localized prostatic cancer treated with radical prostatectomy. J Urol 1988; 139: 766.PubMedGoogle Scholar
  90. 90.
    Schroder FH, van der Cruijsen-Koeter I, de Koning HJ, et al. Prostate cancer detection at low prostate specific antigen. J Urol 2000; 163: 806.PubMedCrossRefGoogle Scholar
  91. 91.
    Narayan P, Gajendran V, Taylor SP, et al. The role of transrectal ultrasound-guided biopsy-based staging, preoperative serum prostate-specific antigen, and biopsy Gleason score in prediction of final pathologic diagnosis in prostate cancer. Urology 1995; 46: 205.PubMedCrossRefGoogle Scholar
  92. 92.
    Partin AW, Yoo J, Carter HB, et al. The use of prostate specific antigen, clinical stage and Gleason score to predict pathological stage in men with localized prostate cancer. J Urol 1993; 150: 110.PubMedGoogle Scholar
  93. 93.
    Chybowski FM, Keller JJ, Bergstralh EJ, et al. Predicting radionuclide bone scan findings in patients with newly diagnosed, untreated prostate cancer: prostate specific antigen is superior to all other clinical parameters. J Urol 1991; 145: 313.PubMedGoogle Scholar
  94. 94.
    Lerner SE, Jacobsen SJ, Lilja H, et al. Free, complexed, and total serum prostate-specific antigen concentrations and their proportions in predicting stage, grade, and deoxyribonucleic acid ploidy in patients with adenocarcinoma of the prostate. Urology 1996; 48: 240.PubMedCrossRefGoogle Scholar
  95. 95.
    Noldus J, Graefen M, Huland E, et al. The value of the ratio of free-to-total prostate specific antigen for staging purposes in previously untreated prostate cancer. J Urol 1998; 159: 2004.Google Scholar
  96. 96.
    Graefen M, Hammerer P, Henke R-P, Hilz H, Huland E, Huland H. Percentage of free PSA does not correlate with pathological outcome. J Urol 1996; 155: 370A.Google Scholar
  97. 97.
    Henricks WH, England BG, Giacherio DA, Oesterling JE, Wojno KJ. Free total PSA-ratio does not predict extraprostatic spread of prostatic adenocarcinoma. J Urol 1996; 155: 369A.Google Scholar
  98. 98.
    Bangma CH, Kranse R, Blijenberg BG, Schroder FH. The free-to-total serum prostate specific antigen ratio for staging prostate carcinoma. J Urol 1997; 157: 544–547.PubMedCrossRefGoogle Scholar
  99. 99.
    Pannek J, Subong EN, Jones KA, et al. The role of free/total prostate-specific antigen ratio in the prediction of final pathologic stage for men with clinically localized prostate cancer. Urology 1996; 48: 51–54.PubMedCrossRefGoogle Scholar
  100. 100.
    Elgamal AA, Comillie FJ, Van Poppel HP, Van de Voorde WM, McCabe R, Baert LV. Free-to-total prostate specific antigen ratio as a single test for detection of significant state Tlc prostate cancer. J Urol 1996;156:1042–1049; discussiom 1047–1049.Google Scholar
  101. 101.
    Pannek J, Rittenhouse HG, Chan DW, Epstein JI, Walsh PC, Partin AW. The use of percent free prostate specific positive specific antigen for staging clinically localized prostate cancer. J Urol 1998; 159; 1238–1242.PubMedCrossRefGoogle Scholar
  102. 102.
    Hara I, Miyake H, Hara S, et al. Value of the serum prostate-specific antigen-alpha 1-antichymotrypsin complex and its density as a predictor for the extent of prostate cancer. BJU Int 2001; 88: 53.PubMedCrossRefGoogle Scholar
  103. 103.
    Kuriyama M, Ueno K, Uno H, et al. Clinical evaluation of serum prostate-specific antigen-alpha1- antichymotrypsin complex values in diagnosis of prostate cancer: a cooperative study. Int J Urol 1998; 5: 48.PubMedCrossRefGoogle Scholar
  104. 104.
    Graefen M, Haese A, Pichlmeier U, et al. A validated strategy for side specific prediction of organ confined prostate cancer: a tool to select for nerve sparing radical prostatectomy. J Urol 2001; 165: 857.PubMedCrossRefGoogle Scholar
  105. 105.
    Haese A, Becker C, Noldus J, et al. Human glandular kallikrein 2: a potential serum marker for predicting the organ confined versus non-organ confined growth of prostate cancer. J Urol 2000; 163: 1491.PubMedCrossRefGoogle Scholar
  106. 106.
    Haese A, Graefen M, Steuber T, et al. Human glandular kallikrein 2 levels in serum for discrimination of pathologically organ-confined from locally advanced prostate cancer in total PSA-levels below 10 ng/ml. Prostate 2001; 49: 101.PubMedCrossRefGoogle Scholar
  107. 107.
    Recker F, Kwiatkowski MK, Piironen T, et al. Human glandular kallikrein as a tool to improve discrimination of poorly differentiated and non-organ-confined prostate cancer compared with prostate-specific antigen. Urology 2000; 55: 481.PubMedCrossRefGoogle Scholar
  108. 108.
    Lange PH, Ercole CJ, Lightner DJ, et al. The value of serum prostate specific antigen determinations before and after radical prostatectomy. J Urol 1989; 141: 873.PubMedGoogle Scholar
  109. 109.
    Haese A, Huland E, Graefen M, et al. Ultrasensitive detection of prostate specific antigen in the followup of 422 patients after radical prostatectomy. J Urol 1999; 161: 1206.PubMedCrossRefGoogle Scholar
  110. 110.
    Stamey TA, Graves HC, Wehner N, et al. Early detection of residual prostate cancer after radical prostatectomy by an ultrasensitive assay for prostate specific antigen. J Urol 1993; 149: 787.PubMedGoogle Scholar

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© Springer Science+Business Media New York 2004

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  • Alexander Haese
  • Alan W. Partin

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