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Tumor Antigen Discovery With T Cells

  • Chapter
Handbook of Cancer Vaccines

Part of the book series: Cancer Drug Discovery and Development ((CDD&D))

Abstract

Studies first carried out in the early 1980s demonstrated that incubation of tumorinfiltrating lymphocytes (TILs) with high doses of interleukin-2 (IL-2) resulted in the generation of CD8+ cytotoxic T cells (CTL) that recognized tumor cells in a major histocompatibility complex (MHC)-restricted manner. This procedure resulted in the generation of tumor-reactive T cells from about 50% of patients with melanoma and a smaller percentage of patients bearing other malignancies. The in vitro sensitization of peripheral blood mononuclear cells (PBMCs) from cancer patients with autologous or human leukocyte antigen (HLA)-matched allogeneic tumor cells in mixed lymphocyte tumor cultures (MLTCs) also resulted in the generation of tumor-reactive T cells. These cells as well as the tumor-reactive TIL-lysed tumor and released a variety of cytokines including interferon-γ (IFN-γ), granulocyte macrophage colony-stimulating factor (GM-CSF), or tumor necrosis factor-α (TNF-α) in response to human leukocyte antigen (HLA)-matched tumor targets. Tumor-reactive class II restricted T cells have also been identified in a significant percentage of the cultures of sensitized PBMC or TIL cultures containing class I—restricted tumor-reactive T cells.

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  1. Paul F. Robbins PhD
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Editors and Affiliations

  1. Duke University Medical Center, Durham, NC, USA

    Michael A. Morse MD , Timothy M. Clay PhD  & H. Kim Lyerly MD ,  & 

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Robbins, P.F. (2004). Tumor Antigen Discovery With T Cells. In: Morse, M.A., Clay, T.M., Lyerly, H.K. (eds) Handbook of Cancer Vaccines. Cancer Drug Discovery and Development. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-680-5_4

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  • DOI: https://doi.org/10.1007/978-1-59259-680-5_4

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4684-9818-9

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