Abstract
Previous chapters have dealt with individual parts of the LC system and with various symptoms that require troubleshooting: leaks, abnormal pressure, changes in the chromatogram, and so on. The various underlying problems suggested by these symptoms can, in most cases, affect quantitative analysis. That is, calculated concentrations for various sample components can be in error as a result of system malfunction of various kinds. In this chapter we will examine the relationship between various LC-system problems and quantitative analysis. We will also look at errors in reported results as another symptom of some underlying problem. This will in turn allow us to use the assay results from a particular LC procedure as a further diagnostic aid in troubleshooting. Before continuing in this chapter, review the basics of LC quantitation in reference (1).
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References
Snyder, L. R. and Kirkland, J. J. (1979) Introduction to Modern Liquid Chromatography, 2nd edn., Wiley-Interscience, New York, Chap. 13.
Snyder, L. R. and van der Wal, Sj. (1981) Anal. Chem. 53, 877.
Webb, P. A., Ball, D., and Thornton, T. (1983) J. Chromatogr. Sci. 21, 47.
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© 1989 Springer Science+Business Media New York
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Dolan, J.W., Snyder, L.R. (1989). Problems in Quantitation. In: Troubleshooting LC Systems. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-640-9_16
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DOI: https://doi.org/10.1007/978-1-59259-640-9_16
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-4757-6879-4
Online ISBN: 978-1-59259-640-9
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