Abstract
In Chapter 14 we discussed the varying causes of misshaped bands: tailing bands, fronting bands, and other kinds of non-Gaussian bands. Odd-shaped peaks are easy to spot by simply glancing at the chromatogram. Closer study of the chromatogram may reveal other kinds of anomalies. For example, we normally expect bandwidth to increase from the beginning to the end of the chromatogram. In other words, the plate numbers of different bands in the chromatogram should be roughly constant. Not infrequently, however, one or more bands within the chromatogram will be obviously wider than bands on either side. Sometimes such a band also exhibits tailing, but not always. At other times we may see unexpected peaks in the chromatogram—peaks that do not correspond to any compound known to be both present in the sample and responsive to the detector. These extra peaks often deflect downward from the baseline (negative peaks), rather than upward as in the normal case.
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References
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Dolan, J.W., Snyder, L.R. (1989). Separation Problems. In: Troubleshooting LC Systems. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-640-9_15
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DOI: https://doi.org/10.1007/978-1-59259-640-9_15
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