Rapid Generation of Peptides for N-Terminal Sequence Analysis Using Immobilized Pronase

Abstract

The primary structure of proteins can often be determined by successive enzymatic and/or chemical digestion procedures that generate peptides yielding overlapping sequence data. Such methodology is time-consuming, labor intensive and subject to sample loss due to handling. We have developed a fast and simple protein fragmentation strategy which will generate overlapping peptides and eliminates much sample handling.