Abstract
Successful degradation of the very resistant human complement component C3 (Mr 187,500) with trypsin in the presence of 2-propanol (1) has prompted us to examine the effects of the lower alcohols and acetonitrile during degradation with some commonly used endoproteases. The benefits might be threefold. 1) Secondary proteolytic digestions might be executed directly on the fractions obtained after initial RP-HPLC. 2) Proteolytic cleavage might be restricted to produce fewer and larger fragments. 3) Complete digestion of resistant proteins might be advanced due to denaturation or disaggregation with such organic solvents.
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Reference
Welinder, K. G., and Svendsen, A. (1986) FEBS Lett. 202, 59–62
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© 1987 Springer Science+Business Media New York
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Welinder, K.G. (1987). Proteolytic Cleavage in RP-HPLC Solvents. In: Walsh, K.A. (eds) Methods in Protein Sequence Analysis · 1986. Experimental Biology and Medicine, vol 14. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-480-1_25
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DOI: https://doi.org/10.1007/978-1-59259-480-1_25
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-4757-5826-9
Online ISBN: 978-1-59259-480-1
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