Abstract
To obtain maximum sequence information from minute amounts of protein, it is desirable to perform specific and complete cleavage at a limited number of sites. To date this criterion is met by very few methods. We wish to draw attention to a metalloprotease from Pseudomonas fragi which is specific for the amino side of aspartyl residues. It has proven a valuable tool in our structural work on intracellular proteins. The cleavage pattern differs considerably from other enzymes commonly used.
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Maier, G., Drapeau, G.R., Doenges, KH., Ponstingl, H. (1987). Generation of Starting Points for Microsequencing with a Protease Specific for the Amino Side of Aspartyl Residues. In: Walsh, K.A. (eds) Methods in Protein Sequence Analysis · 1986. Experimental Biology and Medicine, vol 14. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-480-1_24
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DOI: https://doi.org/10.1007/978-1-59259-480-1_24
Publisher Name: Humana Press, Totowa, NJ
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