Abstract
Since the introduction of in vivo electrochemistry in the study of brain neurochemistry, most applicatons have been made within the nigrostriatal dopamine (DA) system. There are many reasons investigators have been so attracted by this brain region: The striatum contains the highest density of DA-containing neuronal terminals of all the brain. This region is also the largest catecholamine (CA) area of the brain with an almost exclusive dopaminergic innervation. It is easily accessible to investigation with carbon electrodes. Finally, many biochemical data are available concerning the metabolism of DA within this area. From the early in vivo electrochemical studies it was learned that the in vivo study of catechol metabolism in the brain is a relatively difficult task, requiring selective and sensitive methods of detection. As a result, we, like other investigators, have attempted to develop an electrochemical method allowing for selective in vivo monitoring of DA metabolism in the striatum (Gonon et al., 1980). This method enables us to study various features of DA metabolism in the striatum (Gonon et al., 1981b, 1984a; Gonon and Buda, 1985; Gonon, this volume), as well as in other dopaminergic regions (Buda et al., 1981). The use of carbon fiber microelectrodes, because of their size and sensitivity, allow for investigation of other catecholaminergic regions, such as those innervated by noradrenaline (NA)-containing neuronal systems.
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References
Adams, R. N. and Marsden, C. A. (1982) Electrochemical Detection Methods for Monoamine Measurements In Vitro aid In Vivo, In Handbook in Psychopharmacology, vol. 15, (L. L. Iversen, S. D. Iversen, and S. H. Snyder, eds.), Plenum, New York.
Aghajanian, G. K. and Cedarbaum, J. M. (1979) Central Noradrenergic Neurons: Interaction of Autoregulatory Mechanisms With Extrinsic Influences in Catecholamines: Basic and Clinical Frontiers vol. 1, (E. Usdin, I. J. Kopin, and J. Barchas, eds.), Pergamon, New York.
Aghajanian, G. K. and Rogawski, M. A. (1983) The physiological role of alpha-adrenoceptors in the CNS: New concepts from single-cell studies. Trends Pharmacol. Sci. 6, 315–317.
Aghajanian, G. K., Cedarbaum, J. M., and Wang, R. Y. (1977) Evidence for norepinephrine-mediated collateral inhibition of locus coeruleus neurons. Brain Res. 136, 570–577.
Amaral, D. G. and Sinnamon, H. M. (1977) The locus coeruleus: Neurobiology of a central noradrenergic nucleus. Prog. Neurobiol. 9, 147–196.
Armstrong, D. M., Ross, C. A., Pickel, V. M., Joh, T. H., and Reis, D. J. (1982) Distribution of dopamine-, noradrenaline-, and adrenaline-containing cell bodies in the rat medulla oblongata: Demonstrated by the immunocytochemical localization of catecholamine biosynthetic enzymes. J. Comp. Neurol. 212, 173–187.
Aston-Jones, G. and Bloom, F. E. (1981a) Activity of norepinephrinecontaining locus coeruleus neurons in behaving rats anticipates fluctuations in the sleep-waking cycle. J. Neurosci. 1, 876–886.
Aston-Jones, G. and Bloom, F. E. (1981b) Norepinephrine-containing locus coeruleus neurons in behaving rats exhibit pronounced responses to non-noxious environmental stimuli. J. Neurosci. 1, 887–900.
Berod, A., Hartman, B. K., Keller, A., Joh, T. H., and Pujol, J. F. (1982) A new double labelling technique using tyrosine hydroxylase and dopamine-ß-hydroxylase immunochemistry: Evidence for dopaminergic cells lying in the pons of the beef brain. Brain Res. 240, 235–243.
Bloom, F. E., Battenberg, E., Rossier, J., Ling, N., and Guillemin, R. (1978) Neurons containing 13-endorphin in rat brain exist separately from those containing enkephalin: Immunocytochemical studies. Proc. Natl. Acad. Sci. USA 75, 1591–1595.
Brownstein, M., Saavedra, J. M., and Palkovits, M. (1974) Norepinephrine and dopamine in the limbic system of the rat. Brain Res. 79, 431–436.
Buda, M., Gonon, F., Cespuglio, R., Jouvet, M., and Pujol, J. F. (1981) In vivo electrochemical detection of catechols in several dopaminergic brain regions of anesthetized rats. Eur. J. Pharmacol. 73, 61–68.
Buda, M., De Simoni, M. G., Gonon, F., and Pujol, J. F. (1983) Catecholamine metabolism in the rat locus coeruleus as studied by in vivo differential pulse voltammetry. I. Nature and origin of contribuors to the oxidation current at +0.1 V. Brain Res. 273, 197–206.
Cedarbaum, J. M., and Aghajanian, G. K. (1977) Catecholamine receptors on locus coeruleus neurons: Pharmacological characterization. Eur. J. Pharmacol. 44, 375–385.
Cedarbaum, J. M. and Aghajanian, G. K. (1978) Afferent projections to the rat locus coeruleus as determined by a retrograde tracing technique. J. Comp. Neurol. 178, 1–16.
Cespuglio, R., Faradji, H., Hahn, Z., and Jouvet, M. (1984) Voltammetric Detection of Brain 5-Hydroxyindolamines by Means of Electrochemically Treated Carbon Fiber Electrodes: Chronic Recordings for Up to One Month With Movable Cerebral Electrodes in the Sleeping or Waking Rat, in Measurements of Neurotransmitter Release in Vivo IBRO Handbook series. Methods in Neurosciences, vol. 6, (C. A. Marsden, ed.), John Wiley, Chichester.
Cheney, D. L., Le Fevre, H. F., and Racagni, G. (1975) Choline acetyltransferase activity and mass fragmentographic measurement of acetylcholine in specific nuclei and tracts of the rat brain. Neuropharmacology 14, 801–809.
Conti, J., Strope, E., Adams, R. N., and Marsden, C. A. (1978) Voltammetry in brain tissue: Chronic recording of stimulated dopamine and 5-hydroxytryptamine release. Life Sci. 23, 2705–2716.
Curet, O., Dennis, T., and Scatton, B. (1985) The formation of deaminated metabolites of dopamine in the locus coeruleus depends upon noradrenergic neuronal activity. Brain Res 335, 297–301.
Dahlström, A., and Fuxe, K. (1964) Evidence for the existence of monoamine-containing neurons in the central nervous system. I. Demonstration of monoamines in the cell bodies of brain stem neurones. Acta Physiol. Scand. 62 (suppl. 232), 1–55.
Dayton, M. A., Brown, J. C., Stutts, K. H., and Wightman, R. M. (1980) Faradaic electrochemistry at microvoltammetric electrodes. Anal. Chem. 52, 946–950.
De Simoni, M. G., Gonon, F., Buda, M., and Pujol, J. F. (1983) Cholinergic control of catechol metabolism in the rat locus coeruleus as studied by in vivo voltammetry. Eur. J. Pharmacol. 95, 65–70.
Dinan, T. G. and Aston-Jones, G. (1984) Acute haloperiodol increases impulse activity of brain noradrenergic neurons. Brain Res. 307, 359–362.
Engberg, G. and Svensson, T. H. (1980) Pharmacological analysis of a cholinergic receptor mediated regulation of brain norepinephrine neurons. J. Neural. Transm. 49, 137–150.
Fekete, M. I. K., Herman, J. P., Kanyicska, B., and Palkovits, M. (1979) Dopamine noradrenaline and 3,4-dihydroxyphenylacetic acid (DOPAC) levels of individual brain nuclei. Effects of haloperiodol and pargyline. J. Neural. Transm. 45, 207–218.
Foote, S. L., Aston-Jones, G., and Bloom, F. E. (1980) Impulse activity of locus coeruleus neurons in awake rats and monkeys is a function of sensory stimulation and arousal. Proc. Natl. Acad. Sci USA 77, 3033–3037.
Foote, S. L., Bloom, F. E., and Aston-Jones, G. (1983) Nucleus locus coeruleus: New evidence of anatomical and physiological specificity. Physiol. Rev. 63, 844–914.
Gonon, F., and Buda, M. J. (1985) Regulation of dopamine release by impulse flow and by autoreceptors as studied by in vivo voltammetry in the rat striatum. Neuroscience 14, 765–774.
Gonon, F., Buda, M., Cespuglio, R., Jouvet, M., and Pujol, F. J. (1980) In vivo electrochemical detection of catechols in the neostriatum of anaesthetized rats: Dopamine or DOPAC? Nature 286, 902–904.
Gonon, F., Fombarlet, C., Buda, M., and Pujol, J. F. (1981a) Improvement of pyrolytic carbon fiber electrodes by electrochemical treatments. Anal. Chem. 53, 1386–1389.
Gonon, F., Buda, M., Cespuglio, R., Jouvet, M., and Pujol, J. F. (1981b) Voltammetry in the striatum of chronic freely moving rats: Detection of catechols and ascorbic acid. Brain Res. 223 69–81.
Gonon, F., Buda, M., De Simoni, G., and Pujol, J. F. (1983a) Catecholamine metabolism in the rat locus coeruleus as studied by in vivo differential pulse voltammetry. II. Pharmacological and behavioral study. Brain Res. 273, 207–216.
Gonon, F., Cespuglio, R., Buda, M., and Pujol, J. F. (1983b) In Vivo Electrochemical Detection of Monoamine Derivatives, in Methods in Biogenic Amine Research, ( S. Parvez, T. Nagatsu, J. Nagatsu, and H. Parvez, eds.), Elsevier, North-Holland.
Gonon, F., Navarre, F., and Buda, M. (1984a) In vivo monitoring of dopamine release in the rat brain with differential normal pulse voltammetry. Anal. Chem. 56, 573–575.
Gonon, F., Buda, M., and Pujol, J. F. (1984b) Treated Carbon Fibre Electrodes For Measuring Catechols and Ascorbic Acid Treated Carbon Fibre Electrodes, in Measurements of Neurotransmitter Release IBRO Handbook series Methods in Neurosciences vol. 6, ( C. A. Marsden, ed.), John Wiley, Chichester.
Groves, P. M. and Wilson, C. J. (1980) Monoaminergic presynaptic axons and dendrites in rat locus coeruleus seen in reconstructions of serial sections. J. Comp. Neurology 193, 853–862.
Grzanna, R. and Molliver, M. E. (1980) The locus coeruleus in the rat: An immunohistochemical delineation. Neuroscience 5, 21–40.
Guyenet, P. G. and Aghajanian, G. K. (1979) Ach substance P and metenk ephalin in the locus coeruleus: Pharmacological evidence for independent sites of action. Eur. J. Pharmacol. 53, 319–328.
Hökfelt, T. K., Fuxe, K., Goldstein, M., and Johansson, O. (1974) Immunohistochemical evidence for the existence of adrenaline neurons in the rat brain. Brain Res. 66, 166–307.
Jouvet, M. (1972) The role of monoamines and acetylcholine-containing neurons in the regulation of the sleep-waking cycle. Ergeb. Physiol. Biol. Chem. Exp. Pharmacol. 64, 166–307.
Kalia, M., Fuxe, K., and Goldstein, M. (1985) Rat medulla oblongata II. Dopaminergic, noradrenergic and adrenergic neurons, nerve fibers and presumptive terminal processes. J. Comp. Neurol. 233, 308–332.
Kobayashi, R. M., Palkovits, M., Kopin, I. J., and Jacobowitz, D. M. (1974) Biochemical mapping of noradrenergic nerves arising from the rat locus coeruleus. Brain Res. 77, 269–279.
Kovach, P. M., Ewing, A. G., Wilson, R. L., and Wightman, R. M. (1984) In vitro comparison of the selectivity of electrodes for in vivo electrochemistry. J. Neurosci. Meth. 10, 215–227.
Lane, R. F., Hubbard, A. T., Fukunaga, K., and Blanchard, R. J. (1976) Brain catecholamines: Detection in vivo by means of differential pulse voltammetry at surface-modified platinum electrodes. Brain Res. 114, 346–352.
Lindvall, O. and Björklund, A. (1978) Organization of Catecholamine Neurons in the Rat Central Nervous System, in Handbook of Psychopharmacology, vol. 9, (L. L. Iversen, S. D. Iversen, and S. H. Snyder, eds.), Plenum, New York.
Ljungdahl, A., Hökfelt, T., and Nilsson, G. (1978) Distribution of substance P-like immunoreactivity in the central nervous system of the rat. I. Cell bodies and nerve terminals. Neuroscience 3, 861–943.
Louilot, A., Buda, M., Gonon, F., Simon, H., Le Moal, M., and Pujol, J. F. (1985) Effect of haloperidol and sulpiride on dopamine metabolism in nucleus accumbens and olfactory tubercule: A study by in vivo voltammetry. Neuroscience 14, 775–782.
Marsden, C. A., Brazell, M. P., and Maidment, N. T. (1984) An Introduction to In Vivo Electrochemistry, in Measurement of Neurotransmitter Release In Vivo IBRO Handbook series Methods in Neurosciences, vol. 16, (C. A. Marsden, ed.), John Wiley, Chichester.
Marwaha, J. and Aghajanian, G. K. (1982) Relative potencies of alpha-1 and alpha-2 antagonists in the locus coeruleus, dorsal raphe and dorsal lateral geniculate nuclei: An electrophysiological study. J. Pharmacol. Exp. Ter. 222, 287–293.
Mermet, C. and Gonon, F. (1986) In vivo voltammetric monitoring of noradrenaline release and catecholamine metabolism in the hypothalamic paraventricular nucleus. Neuroscience,in press.
Milon, H. and McRae-Degueurce, A. (1982) Pharmacological investigation on the role of dopamine in the rat locus coeruleus. Neurosci. Let. 30, 297–301.
Moore, R. Y. and Bloom, F. E. (1979) Central catecholamine neuron systems: Anatomy and physiology of the norepinephrine and epinephrine systems. Ann. Rev. Neurosci. 2, 113–168.
Pickel, V. M., Joh, T. M., Reiss, D. J., Leeman, S. E., and Miller, R. J. (1979) Electron microscopic localization of substance P and enkephalin in axon terminals related to dendrites of catecholaminergic neurons. Brain Res. 160, 387–400.
Ponchon, J. L., Cespuglio, R., Gonon, F., Jouvet, M., and Pujol, J. F. (1979) Normal pulse polarography with carbon fiber electrodes for in vitro and in vivo determination of catecholamines. Anal. Chem. 51, 1483–1486.
Quintin, L., Buda, M., Hilaire, G., Bardelay, C., Ghignone, M., and Pujol, J. F. (1986a) Catecholamine metabolism in the rat locus coeruleus as studied by in vivo differential pulse voltammetry. III: Evidence for the existence of an alpha-2-adrenergic tonic inhibition in behaving rats. Brain Res.,in press.
Quintin, L., Hilaire, G., and Pujol, J. F. (1986b) Variations in DOPAC concentration are correlated to single cell firing changes in the rat locus coeruleus. Neuroscience,in press.
Quintin, L., Gonon, F., Buda, M., Ghignone, M., Hilaire, G., and Pujol, J. F. (1986c) Clonidine modulates locus coeruleus metabolic hyperactivity induced by stress in behaving rats. Brain Res. 362, 366–369.
Ross, M. E., Park, D. H., Teitelman, G., Pickel, V. M., Reis, D.J., and Joh, T. H. (1983) Immunohistochemical localization of choline acetyltransferase using a monoclonal antibody: A radioautographic method. Neuroscience 10, 907–922.
Rotter, A., Birdsall, J. M., Field, P. M., and Raisman, G. (1979) Muscarinic receptors in the central nervous system of the rat. II. Distribution of binding of 3H-propylbenzilylcholine mustard in the midbrain and hindbrain. Brain Res. 1, 167–183.
Salzman, P. M. and Roth, R. H. (1980a) Poststimulation catecholamine synthesis and tyrosine hydroxylase activation in central noradrenergic neurons. I. In vivo stimulation of the locus coeruleus. J. Pharmacol. Exp. Ther. 212, 64–73.
Salzman, P. M. and Roth, R. H. (1980b) Post-stimulation catecholamine synthesis and tyrosine hydroxylase activation in central noradrenergic neurons. II. Depolarized hippocampal slices. J. Pharmacol. Exp. Ther. 212, 74–84.
Sawchenko, P. E. and Swanson, L. W. (1982) The organization of noradrenergic pathways from the brainstem to the paraventricular and supraoptic nuclei in the rat. Brain Res. Rev 4, 275–325.
Scatton, B., Dennis, T., and Curet, O. (1984) Increase in dopamine and DOPAC levels in noradrenergic terminals after electrical stimulation of the ascending noradrenergic pathways. Brain Res. 298, 193–196.
Schwarcz, R., Hökfelt, T., Fuxe, K., Jonsson, G., Goldstein, M., and Terenius, L. (1979) Ibotenic acid-induced neuronal degeneration: A morphological and neurochemical study. Exp. Brain Res 37, 199–216.
Simon, H., Le Moal, M., Stinus, L., and Calas, A. (1979) Anatomical relationships between the ventral mesencephalic tegmentum A 10 region and the locus coeruleus as demonstrated by anterograde and retrograde tracing techniques. J. Neur. Transco. 44, 77–86.
Suaud-Chagny, M. F., Steinberg, R., Mermet, C., and Gonon, F. (1986) In vivo voltammetric monitoring of catecholamine metabolism in Al and A2 areas of the rat medulla oblongata. J. Neurochem.,in press.
Svensson, T. H., and Usdin, T. (1978) Feedback inhibition of brain noradrenaline neurons by tricyclic antidepressants: Alpha-receptor mediation. Science 202, 1089–1091.
Swanson, L. W. (1976) The locus coeruleus: A cytoarchitectonic, golgi and immunohistochemical study in the albino rat. Brain Res. 110, 39–56.
Swanson, L. W. and Hartman, B. K. (1976) The central adrenergic system. An immunofluorescence study of the location of cell bodies and their efferent connections in the rat utilizing dopamine-(3hydroxylase as a marker. J. Comp. Neurol. 163, 467–506.
Versteeg, D. M. G., Van Der Gugten, J., De Jong, W., and Palkovits, M. (1976) Regional concentrations of noradrenaline and dopamine in rat brain. Brain Res. 113, 563–574.
Young III, W. S. and Kuhar, M. G. (1980) Noradrenergic alpha-1 and alpha-2 receptors: Light microscopic autoradiographie localization. Proc. Natl. Acad. Sci. USA 77, 1696–1700.
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Buda, M., Gonon, F.G. (1987). Study of Brain Noradrenergic Neurons by Use of In Vivo Voltammetry. In: Justice, J.B. (eds) Voltammetry in the Neurosciences. Contemporary Neurosciences. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-463-4_7
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