Abstract
Staining procedures, and more specialized histochemistry and immunohistochemistry, are performed to obtain specific types of information about bone cells, bone, or cartilage extracellular matrix (ECM) and bone formation and mineralization. Unfortunately, all these aspects cannot usually be obtained with one staining procedure or localization method, and thus the specific needs of a project must be clearly defined at the outset of specimen preparation. If bone formation and mineralization are important for the study, then the specimens must be processed in an undecalcified method and sectioned with a special microtome and knives. If more routine histologic stains and localizations are the desired outcome, then routine or specialized decalcification procedures can be performed and the specimen can be embedded in paraffin. We have recently reviewed the technical methods elsewhere.9 The objective of this chapter is to present technical procedures for the more commonly used bone stains and localizations.
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References
Anderson C: Manual for the Examination of Bone. CRC Press, Boca Raton, FL, 1982.
Baron R, Vignery A, Neff L, et al: Processing of undecalcified bone specimens for bone histomorphometry. In: Recker RR, ed: Bone Histomorphometry: Techniques and Interpretation. CRC Press, Boca Raton, FL, 1983: 13–35.
Derkx P, Birkenhäger-Frenkel DH: A thionin stain for visualizing bone cells, mineralizing fronts and cement lines in undecalcified bone sections. Biotech Histochem 70: 70–74, 1995.
Gruber HE and Mekikian P: Application of Stains-All for demarcation of cement lines in methacrylate-embedded bone. Biotech Histochem 66: 181–184, 1991.
Gruber HE: Adaptations of Goldner’s Masson trichrome stain for the study of undecalcified plastic embedded bone. Biotech Histochem 67: 30–34, 1992.
Gruber HE, Marshall GJ, Kirchen ME, et al: Improvements in dehydration and cement line staining for methacrylate embedded human bone biopsies. Stain Technol 60: 337–344, 1985.
Gruber HE, Marshall GJ, Nolasco LM, et al: Alkaline and acid phosphatase demonstration in human bone and cartilage: effects of fixation intervals and methacrylate embedments. Stain Technol 63: 299–306, 1988.
Gruber HE, Stasky AA: Large specimen bone embedment and cement line staining. Biotech Histochem 72: 198–201, 1997.
Gruber HE, Stasky AA: Histologic study in orthopaedic animal research. In: An YH, Friedman, RJ, eds: Animal Models in Orthopaedic Research. CRC Press, Boca Raton, FL, 1999: 115–138.
Hermanns W, Colbatzky F, Gunther A, Steiniger B: Ia antigens in plastic-embedded tissues: A post-embedding immunohistochemical study. J Histochem Cytochem 34: 827–831, 1986.
Larsson L-I: Immunocytochemistry: Theory and Practice. CRC Press, Boca Raton, FL, 1988.
Lucena SB, Duarte MEL, Fonseca EC: Plastic embedded undecalcified bone biopsies: an immunohistochemical method for routine study of bone marrow extracellular matrix. J Histotech 20: 253–257, 1997.
Malluche HH, Faugere M-C: Atlas of Mineralized Bone Histology. S. Karger AG, Basel, Switzerland, 1986.
Myers JD: Development and application of immunocytochemical staining techniques: A review. Diagn Cytopathol 5: 318–330, 1989.
Page KM: Bone and the preparation of bone sections. In: Bancroft JD, Stevens A, eds: Theory and Practice of Histological Techniques. Churchill Livingstone, London, 1982: 297–331.
Schenk RK, Olah Ai, Herrmann W: Preparation of calcified tissues for light microscopy. In: Dickson GR, ed: Methods of Calcified Tissue Preparation. Elsevier, Amsterdam, 1984: 1–78.
Shi S-R, Cote RJ, Chen T, et al: Antigen retrieval technique: an important approach to standardization of immunohistochemistry. Cell Vision 3: 235–236, 1996.
Shi SR, Cote RJ, Taylor CR: Antigen retrieval immunohistochemistry: past, present, and future. J Histochem Cytochem 45: 327–343, 1997.
Tacha DE, Bowman PD, McKinney L: High resolution light microscopy and immunocytochemistry with glycol methacrylate embedded sections and immunogold-silver staining. J Histotech 16: 13–18, 1993.
Taylor CR, Shi SR, Chen C, et al: Comparative study of antigen retrieval heating methods: microwave, microwave and pressure cooker, autoclave, and steamer. Biotech Histochem 71: 263–270, 1996.
Tsuji Y, Kusuzaki K, Hirasawa Y, et al: Ki-67 antigen retrieval in formalin-or ethanol-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining with autoclave treatment. Acta Histochem Cytochem 30: 251–255, 1997.
Villaneuva AR, Hattner RS, Frost HM: A tetrachrome stain for fresh mineralized bone sections. Stain Technol 39: 87–94, 1964.
Wakamatsu K, Ghazizadeh M, Ishizaki M, et al: Optimizing collagen antigen unmasking in paraffin-embedded tissues. Histochem J 29: 65–72, 1997.
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Gruber, H.E., Ingram, J.A. (2003). Basic Staining and Histochemical Techniques and Immunohistochemical Localizations Using Bone Sections. In: An, Y.H., Martin, K.L. (eds) Handbook of Histology Methods for Bone and Cartilage. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-417-7_20
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DOI: https://doi.org/10.1007/978-1-59259-417-7_20
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61737-277-3
Online ISBN: 978-1-59259-417-7
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