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Infiltration Techniques and Results in Different Types of Resin

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Handbook of Histology Methods for Bone and Cartilage

Abstract

Plastic-embedding technique generally does not require the removal of the resin before staining, a process that could introduce artifacts at the tissue-implant interface. The presence of the resin in the sections makes the staining procedures different from routine paraffin-embedded tissues, and achieving satisfactory staining is more difficult. An exception is methyl methacrylate (MMA) which is removed from the sections after cutting to permit staining. Embedding in MMA requires the removal of the resin with solvents. Glycol methacrylate cannot be removed because of the high number of crosslinking binding sites present in the chains of the glycol methacrylate polymer.7,8

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Scarano, A., Orsini, G., Piattelli, A. (2003). Infiltration Techniques and Results in Different Types of Resin. In: An, Y.H., Martin, K.L. (eds) Handbook of Histology Methods for Bone and Cartilage. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-417-7_12

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  • DOI: https://doi.org/10.1007/978-1-59259-417-7_12

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61737-277-3

  • Online ISBN: 978-1-59259-417-7

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