Abstract
Embedding techniques were first developed in the mid 1800s in response to the significant improvements in light microscopy. As the resolution of microscopy increased, so did the need for improved quality of the tissue specimens to be analyzed. The specimens needed to be cut in much thinner slices, which could only be done if embedded in a suitable medium supporting the material and providing the hardness required for thinner sectioning. Edwin Klebs introduced the paraffin-embedding methodology in 1869.18 However, at that time, the material was not completely infiltrated with paraffin and the sections were cut freehand with a razor.
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An, Y.H., Moreira, P.L., Kang, Q.K., Gruber, H.E. (2003). Principles of Embedding and Common Protocols. In: An, Y.H., Martin, K.L. (eds) Handbook of Histology Methods for Bone and Cartilage. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-417-7_11
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DOI: https://doi.org/10.1007/978-1-59259-417-7_11
Publisher Name: Humana Press, Totowa, NJ
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