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Introduction to Experimental Bone and Cartilage Histology

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Book cover Handbook of Histology Methods for Bone and Cartilage

Abstract

Experimental and diagnostic histotechnology and histomorphometry are two consecutive steps that are essential for clinical work and research in bone or cartilage. Unlike routine histological or pathological laboratories, a laboratory for bone-tissue processing has unique characteristics, such as more demanding fixation, the challenging process of decalcification, media infiltration and embedding, the need for heavy-duty microtomes, diamond circular or wire saws for tissue sectioning, and grinders or grinding machines for section thinning and grinding. The existence of metal or other implants adds further complexity to the process. In addition to the routine microscopic evaluation of cellular and structural features, histomorphometry is a unique facet of bone and cartilage histology. A laboratory engaged in experimental bone and cartilage histology should have both basic and specialized equipment for its unique needs. Histotechnology and histomorphometry are both techniques and arts. They require patience and skilled hands as well as basic biological and anatomical knowledge at the level of organs, tissues, cells, and even molecules. Pre-employment and continuing education are necessary for maintaining adequate knowledge of biological tissues, basic and specific histological techniques, good laboratory practice, and laboratory safety issues.

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Authors and Affiliations

  1. Orthopaedic Research Laboratory, Medical University of South Carolina, Charleston, SC, USA

    Yuehuei H. An

  2. Orthopaedic Research Biology, Carolinas Medical Center, Charlotte, NC, USA

    Helen E. Gruber

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  1. Yuehuei H. An
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Editors and Affiliations

  1. Department of Orthopaedic Surgery, Medical University of South Carolina, Charleston, SC, USA

    Yuehuei H. An MD (Associate Professor, Director of Orthopaedic Research Laboratory, Assistant Professor of Bioengineering) (Associate Professor, Director of Orthopaedic Research Laboratory, Assistant Professor of Bioengineering)

  2. Department of Bioengineering, Clemson University, Clemson, SC, USA

    Yuehuei H. An MD (Associate Professor, Director of Orthopaedic Research Laboratory, Assistant Professor of Bioengineering) (Associate Professor, Director of Orthopaedic Research Laboratory, Assistant Professor of Bioengineering)

  3. Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, Medical University of South Carolina, Charleston, SC, USA

    Kylie L. Martin BSc (Histological Technician and Laboratory Manager) (Histological Technician and Laboratory Manager)

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© 2003 Springer Science+Business Media New York

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An, Y.H., Gruber, H.E. (2003). Introduction to Experimental Bone and Cartilage Histology. In: An, Y.H., Martin, K.L. (eds) Handbook of Histology Methods for Bone and Cartilage. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-417-7_1

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  • DOI: https://doi.org/10.1007/978-1-59259-417-7_1

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61737-277-3

  • Online ISBN: 978-1-59259-417-7

  • eBook Packages: Springer Book Archive

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