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Cardiac Markers pp 173–185Cite as

Antibody Selection Strategies in Cardiac Troponin Assays

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Part of the book series: Pathology and Laboratory Medicine ((PLM))

Abstract

The history of troponin assays starts from the late 1980s. In 1987, B. Cummins reported a new analyte that could be used for diagnosis of acute myocardial infarction (MI) (1). The new method was based on the immunodetection of cardiac isoform of troponin I (cTnI). Two years later Katus and colleagues (2) suggested utilization of cardiac troponin T (cTnT) as a cardiac marker. Today dozens of commercial cTnI assays are available. Troponins are the most “popular” cardiac markers. According to Apple et al. (3), in 1999 about 85% of clinical laboratories in the United States were using this analyte in their practice. But our knowledge about the nature of cTnI circulating in the blood is still only the tip of an iceberg. Limited knowledge of the antigen limits the possibilities of developing a theory of cTnI assays, and as a consequence results in huge between-method variations for existing cTnI assays (4,5). Obviously the lack of an international standard (6) complicates assay standardization, but in the case of cTnI, the antibody standardization can be even more important (4).

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  1. Alexei Katrukha
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  1. Department of Pathology and Laboratory Medicine, Hartford Hospital, Hartford, CT, USA

    Alan H. B. Wu PhD

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Katrukha, A. (2003). Antibody Selection Strategies in Cardiac Troponin Assays. In: Wu, A.H.B. (eds) Cardiac Markers. Pathology and Laboratory Medicine. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-385-9_10

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  • DOI: https://doi.org/10.1007/978-1-59259-385-9_10

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61737-319-0

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