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Mutagenesis Studies of Mammalian Lipoxygenases

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Molecular and Cellular Basis of Inflammation

Part of the book series: Current Inflammation Research ((CIRES))

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Abstract

A widely used assay in studies of lipoxygenases (LOs) is the oxygénation of arachidonic acid, and, to date, mammalian enzymes that introduce oxygen at C-5, C-8, C-12, and C-15 of the arachidonic acid backbone have been described. Oxygénation of arachidonic acid is a physiological function, e.g., for 5-LO in leukotriene biosynthesis. However, it is possible that these enzymes also have other roles, one indication is the low activity of mouse epidermal 12-LO regarding oxygénation of free fatty acids (1).

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  1. Olof Rådmark
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Editors and Affiliations

  1. Department of Anesthesia, Center for Experimental Therapeutics and Reperfusion Injury, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA, USA

    Charles N. Serhan

  2. Department of Pathology, University of Michigan Medical School, Ann Arbor, MI, USA

    Peter A. Ward

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Rådmark, O. (1999). Mutagenesis Studies of Mammalian Lipoxygenases. In: Serhan, C.N., Ward, P.A. (eds) Molecular and Cellular Basis of Inflammation. Current Inflammation Research. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-253-1_4

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  • DOI: https://doi.org/10.1007/978-1-59259-253-1_4

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