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The Fixation of Chemical Forms on Nitrocellulose Membranes

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Book cover Methods in Plant Electron Microscopy and Cytochemistry

Abstract

Tissue printing is becoming a general procedure for the biochemical, molecular, anatomical, and physiological characterization of biological specimens and plant natural products. Tissue printing dates back to 1957, when Daoust (1) reported a substrate film printing. Tissue printing is the art and science of visualization of soluble materials and information that are transferred to membranes such as nitrocellulose membrane. In the 1960s, Daoust (2) used the technique to localize protease, amylase, RNase and DNase by placing cryostat sections of various animal organs (liver, kidney, pancreas, and intestine) on substrate films of gelatin, starch, or gelatin-nucleic acid, respectively. When the films were incubated for a few minutes and then stained for the substrate, negative images were obtained.

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References

  1. Daoust R. Localization of deoxyribonuclease in tissue sections. A new approach to the histochemistry of enzymes. Exp Cell Res 1957; 12: 203–211.

    Article  PubMed  CAS  Google Scholar 

  2. Daoust R. Histochemical localization of enzyme activities by substrate film methods: Ribonucleases, proteases, amalyses and hyaluronidase. Int Rev Cytol 1965; 18: 191–221.

    Article  PubMed  CAS  Google Scholar 

  3. Cassab GI, Varner JE. Immunocytolocalization of extensin in developing soybean seed coats by immunogold-silver staining and by tissue printing on nitrocellulose paper. J Cell Biol 1987; 105: 2581–2588.

    Article  PubMed  CAS  Google Scholar 

  4. Antikainen M, Griffith M, Zhang J, Hon WC, Yang DSC, Pihakashi-Maunsbach K. Immunolocalization of antifreeze proteins in winter rye leaves, crowns, and roots by tissue printing. Plant Physiol 1996; 1.10: 845–857.

    Google Scholar 

  5. Balsalobre JM., Mas P, Sanchez-Pina MA, Pallas V. Spatial distribution of acidic chitinases and their messenger RNAs in tobacco plants infected with cherry leaf roll virus. Mol Plant Microb Interact 1997; 10: 784–788.

    Article  CAS  Google Scholar 

  6. Conley CA, Hanson MR. Cryostat tissue printing: An improved method for histochemical and immunocytochemical localization in soft tissues. Biotechniques 1997; 22: 490–496.

    Google Scholar 

  7. Cho HT, Kende H. Expansions and internodal growth of deepwater rice. Plant Physiol 1997; 113: 1145–1151.

    Article  PubMed  CAS  Google Scholar 

  8. Cohen JD, Illic N, Taylor R, Dunlap JR, Slovin JP. Cucurbitaceaes: evaluation and enhancement in cucurbit germplasm, in Cucurbitaceae (Lester CE, Dunlop JR, eds.), Gateway, 1995, pp. 104–109.

    Google Scholar 

  9. Helguera MA. Immunological detection of a GarV-type virus in Argentine garlic cultivars. Plant Dis 1997; 81: 1005–1010.

    Article  CAS  Google Scholar 

  10. Henderson J, Bauly JM, Ashford DA, Oliver SC, Hawes CR, Lazarus CM, Venis MA, Napier RM. Retention of maize auxin-binding protein in the endoplasmic reticulum: quantifying escape and the role of auxin. Planta 1997; 202: 313–323.

    Article  PubMed  CAS  Google Scholar 

  11. Hood KR, Baasiri RA, Fritz SE, Hood EE. Biochemical and tissue print analyses of hydroxyproline-rich glycoproteins in cell walls of sporophytic maize tissues. Plant Physiol 1991; 96: 1214–1219.

    Article  PubMed  CAS  Google Scholar 

  12. Hilal M, Castagnaro A, Moreno H, Massa EM. Specific localization of the respiratory alternative oxidase in meristematic and xylematic tissues from developing soybean roots and hypocotyls. Plant Physiol 1997; 115: 1499–1503.

    Article  PubMed  CAS  Google Scholar 

  13. Hu JS, Sether DM, Liu XP, Wang M, Zee F, Ullman DE. Use of a tissue blotting immunoassay to examine the distribution of pineapple Clostero virus in Hawaii. Plant Dis 1997; 81: 1150–1154.

    Article  Google Scholar 

  14. Jacinto T, McGurl B, Franceschi V, Felano-Freier J, Ryan CA. Tomato prosystemin promoter confers would-inducible, vascular bundle-specific expression of the betaglucuronidase gene in transgenic tomato plants. Planta 1997; 203: 406–412.

    Article  CAS  Google Scholar 

  15. Jinn TL, Chang PFL, Chen YM, Key JL, Lin CY. Tissue-type-specific-heat-shock response and immunolocalization of class 1 low-molecular-weight heat-shock proteins in soybean. Plant Physiol 1997; 14: 429–438.

    Google Scholar 

  16. Kausch KD, Handa AK. Molecular cloning of a ripening-specific lipoxygenase and its expression during wild-type and mutant tomato fruit development. Plant Physiol 1997; 113: 1041–1050.

    Article  PubMed  CAS  Google Scholar 

  17. Key JL, Lin CY. Tissue-type-specific heat-shock response and immunolocalization of class 1 low-molecular-weight heat-shock proteins in soybean. Plant Physiol 1997; 114: 429–438.

    PubMed  Google Scholar 

  18. Mas P, Pallas V. Long-distance movement of cherry leaf roll virus in infected tobacco plants. J Gen Virol 1996; 77: 531–540.

    Article  PubMed  CAS  Google Scholar 

  19. Roth R, Boudet AM, Pont-Lezica R. Lignification and cinnamyl alcohol dehydrogenase activity in developing stems of tomato and popular: a spatial and kinetic study through tissue printing. J Exp Bot 1997; 48: 247–254.

    Article  CAS  Google Scholar 

  20. Pereira S, Pissarra J, Sunkel C, Salema R. Tissue-specific distribution of glutamine synthetase in potato tubers. Ann Bot 1996; 77: 429–432.

    Article  CAS  Google Scholar 

  21. Ramalho-Santos M, Pissarra J, Verissimo P, Pereira S, Salema R, Pires E, Faro CJ., Cardosin A, An abundant aspartic proteinase, accumulates in protein storage vacuoles in the stigmatic papillae of Cynara cardunculus I. Planta 1997; 203: 204–212.

    Article  PubMed  CAS  Google Scholar 

  22. Taylor R, Varner JE. The role of calcium in maintaining cell-wall firmness studied by physical printing on nitrocellulose membranes, in Tissue Printing: Tools for the Study of Anatomy, Histochemistry, and Gene Expression (Reid P, Pont-Lezica R, del Campillo E, Taylor R, eds.), Academic Press, New York, 1992, pp. 15–17.

    Google Scholar 

  23. Taylor R, Varner JE. Ascorbic acid location by tissue printing on nitrocellulose, in Tissue Printing: Tools for the Study ofAnatomy, Histochemistry, and Gene Expression (Reid P, Pont-Lezica R, del Campillo E, Taylor R, eds.), Academic Press, New York, 1992, pp. 163–164.

    Google Scholar 

  24. Taylor R, Bailey BA, Dean JFD, Anderson JD. Translocation of fungal protein in plants, in Tissue Printing: Tools for the Study of Anatomy, Histochemistry, and Gene Expression (Reid P, Pont-Lezica R, del Campillo E, Taylor R, eds.), Academic Press, New York, 1992, pp. 43–58.

    Google Scholar 

  25. Varner JE, Ye Z. Tissue printing. FASEB J 1994; 8: 378–384.

    PubMed  CAS  Google Scholar 

  26. Al-Wandawi H. Chemical composition of seeds of two okra cultivars [Emerald, Ibtaria, Abelmoschus esculentus]. J Agric Food Chem 1983; 31: 1355–1358.

    Article  PubMed  CAS  Google Scholar 

  27. Ames JM, MacLeod G. Volatile components of okra, in Phytochemistry 1990; 29: 1201–1207.

    Article  CAS  Google Scholar 

  28. Baxter L, Waters L Jr. Chemical changes in okra stored in air and controlled atmosphere. J Am Soc Hortic Sci 1990; 115: 452–454.

    CAS  Google Scholar 

  29. Ndjouenkeu R, Goycoolea FM, Morris ER, Akingbala JO. Rheology of okra (Hibiscus esculentus L.) and dika nut (Irvingia gabonensis) polysaccharides. Carbohydr Polvm 1996; 29: 263–269.

    Article  CAS  Google Scholar 

  30. Bryant LA, Montecalvo J Jr, Moren KS, Loy, B. Processing, functional, and nutritional properties of okra seed product. J Food Sci Off Publ Inst Food Technol 1988; 53: 810–816.

    Google Scholar 

  31. Moawas FG, Wahab EI, Shehata FW, Abdel-Naiem FM. Preparation of some chemical components from okra stalks. Ann Agric Sci Moshtohor 1984; 21: 603–612.

    Google Scholar 

  32. Cook DA, Brown A III. Somatic embryogenesis and organogenesis in okra (Abelmoschus esculentus L. Moench.) Somatic Embryogenesis and Synthetic Seed 1995; 31: 164–169.

    Article  Google Scholar 

  33. Bialek K, Cohen JD. Isolation and partial characterization of the major amidelinked conjugate of indole-3-acetic acid from Phaseolus vulgaris. Plant Physiol 1986; 80: 99–104.

    Article  PubMed  CAS  Google Scholar 

  34. Sambrook J, Fritsch EF, Mantiatis T. Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1989, pp. 1867–1868.

    Google Scholar 

  35. Taylor R. Demonstration of tissue printing; an overview, in Tissue Printing: Tools for the Study of Anatomy, Histochemistry and Gene Expression (Reid P, Pontlezica R, del Campillo E, Taylor R, eds.), Academic Press, New York, 1992, pp. 5–7.

    Google Scholar 

  36. Taylor R, Inamine G, Anderson JD. Tissue printing as a tool for observing immunological and protein profiles in young and mature celery petioles. Plant Physiol 1993; 102: 1027–1031.

    PubMed  CAS  Google Scholar 

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Authors
  1. Rosannah Taylor
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Editors and Affiliations

  1. Mary Baldwin College, Richmond, VA, USA

    William V. Dashek

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© 2000 Springer Science+Business Media New York

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Taylor, R. (2000). The Fixation of Chemical Forms on Nitrocellulose Membranes. In: Dashek, W.V. (eds) Methods in Plant Electron Microscopy and Cytochemistry. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-232-6_7

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  • DOI: https://doi.org/10.1007/978-1-59259-232-6_7

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61737-199-8

  • Online ISBN: 978-1-59259-232-6

  • eBook Packages: Springer Book Archive

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