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Methods for the Detergent Release of Particle-Bound Plant Proteins

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Methods in Plant Electron Microscopy and Cytochemistry

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Abstract

There are three major classes of membrane proteins, i.e., peripheral or extrinsic proteins, integral or intrinsic proteins and transport proteins (1). The peripheral proteins (see Fig. 1) appear to be restricted to either the outer or inner layers of the membrane bilayer. These proteins, which are bound chiefly by ionic forces to the polar heads of phospholipids (electrostatic bonding), can be isolated through alteration of the pH or ionic strength of the suspension medium. Integral or transmembrane proteins are linked either electrostatically or by means of biophysical lipophilicity to the inner domains of the membrane bilayer (1). Integral proteins appear to be anchored in the membrane bilayer via polar amino acid sequences (2). There are two types of integral proteins, simple and complex. Whereas the former possess γ helical structure (3), the latter are globular and comprised of several y helical loops that may traverse the membrane more than once (multiple hairpin bending). The transport proteins may be either of the peripheral or integral type consisting of pumps, carriers, and channels based on energy input (1). Thorough treatments of various aspects of membrane proteins can be found in Martanosi (4), Azzi (5), Gennis (6), Turner (7), Shinitzky (8), Lee (9), Papa and Tager (10), Findlay (11), and Van Heijne (12).

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  1. William V. Dashek
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  1. Mary Baldwin College, Richmond, VA, USA

    William V. Dashek

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Dashek, W.V. (2000). Methods for the Detergent Release of Particle-Bound Plant Proteins. In: Dashek, W.V. (eds) Methods in Plant Electron Microscopy and Cytochemistry. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-232-6_12

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  • DOI: https://doi.org/10.1007/978-1-59259-232-6_12

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61737-199-8

  • Online ISBN: 978-1-59259-232-6

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