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Gene Engineering in Endocrinology pp 217–237Cite as

Transgenic Approaches to Study Developmental Expression and Regulation of the Gonadotropin Genes

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Part of the book series: Contemporary Endocrinology ((COE,volume 22))

Abstract

In the past decade, transgenic animals have evolved as key tools for studies of the development, molecular biology, and function of the reproductive system. Probably the single most important contribution of transgenics to the investigation of gonadotrophs and the gonadotropin genes has been the development through targeted oncogenesis of differentiated pituitary gonadotroph cell lines, specifically the α-T3 (1) and LβT2 (2) cell lines. This chapter reviews the application of these cell lines and in vivo transgenic expression systems to the understanding of the physiological regulation of the glycoprotein hormone α-subunit, luteinizing hormone β (LHβ), and follicle-stimulating hormone β (FSHβ) genes, and the detailed analysis of promoter elements involved in the complex integration of signals for α-subunit gene transcription. Other advances that have been possible as a direct result of the α-T3 cell line include the cloning of LH-2, a novel LIM/homeodomain transcription factor (3), and the gonadotropin-releasing hormone (GnRH) receptor cDNAs (4,5), as well as a detailed ding of the intracellular signaling pathways involved in GnRH action (for a review see ref. 6).

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Authors
  1. Kathryn E. Graham MD
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  2. Malcolm J. Low MD, PhD
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Editor information

Editors and Affiliations

  1. Division of Endocrinology, Department of Internal Medicine, University of Virginia Health Sciences Center, Charlottesville, VA, USA

    Margaret A. Shupnik PhD

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© 2000 Springer Science+Business Media New York

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Graham, K.E., Low, M.J. (2000). Transgenic Approaches to Study Developmental Expression and Regulation of the Gonadotropin Genes. In: Shupnik, M.A. (eds) Gene Engineering in Endocrinology. Contemporary Endocrinology, vol 22. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-221-0_11

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  • DOI: https://doi.org/10.1007/978-1-59259-221-0_11

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61737-147-9

  • Online ISBN: 978-1-59259-221-0

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