Abstract
In 1937 Tiselius (1) reported on a methodology that was to become one of the most novel ways by which proteins could be separated from one another. His apparatus, although large in size relative to current systems, established the serum protein profile still used today. The apparatus, α U-tube, separated the serum proteins in the liquid phase into five fractions, albumin, α-1, α-2, β, and γ globulins. Through the years chemical pathologists and clinical chemists have refined both the methodology and the apparatus. The driving forces behind the development of newer modifications and innovations to his methodology was dependent on:
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1.
Building a more compact and less expensive form of instrument.
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Recording the resultant separation, on solid, visible media that could be stored, and that was capable of visual interpretation.
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Densitometric scanning, so that semi-quantitative values might be assigned to the separated components.
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4.
Making the apparatus available through commercial channels to make it widely available.
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Jolliff, C.R. (2001). Clinical Serum Protein Capillary Zone Electrophoresis. In: Petersen, J.R., Mohammad, A.A. (eds) Clinical and Forensic Applications of Capillary Electrophoresis. Pathology and Laboratory Medicine. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-120-6_4
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DOI: https://doi.org/10.1007/978-1-59259-120-6_4
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