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Capillary Electrophoresis-Mass Spectrometry of Biologically Active Peptides and Proteins

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Clinical and Forensic Applications of Capillary Electrophoresis

Part of the book series: Pathology and Laboratory Medicine ((PLM))

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Abstract

Capillary electrophoresis (CE) is characterized by rapid analysis times and ultra-high resolution capabilities. Indeed, these characteristics, in conjunction with low sample consumption and improved analyte recovery, were expected to revolutionize the analysis of complex biological mixtures of peptides and proteins (1–3). Furthermore, the family of CE separation techniques affords numerous other separation mechanisms that differ from high-performance liquid chromatography (HPLC) separation of peptides and proteins. In addition to free solution separations such as capillary zone electrophoresis (CZE) (4),analyte separation of peptides and proteins can also be effected by isoelectric focusing (IEF) (5–8), isotachophoresis (9), molecular weight sieving (10), and micellar electrokinetic chromatography (MEKC) (11).

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Authors
  1. Stephen Naylor
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  2. Andy J. Tomlinson
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Editors and Affiliations

  1. University of Texas Medical Branch, Galveston, TX, USA

    John R. Petersen  & Amin A. Mohammad  & 

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© 2001 Springer Science+Business Media New York

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Naylor, S., Tomlinson, A.J. (2001). Capillary Electrophoresis-Mass Spectrometry of Biologically Active Peptides and Proteins. In: Petersen, J.R., Mohammad, A.A. (eds) Clinical and Forensic Applications of Capillary Electrophoresis. Pathology and Laboratory Medicine. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-120-6_16

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  • DOI: https://doi.org/10.1007/978-1-59259-120-6_16

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61737-113-4

  • Online ISBN: 978-1-59259-120-6

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