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Combining Capillary Electrophoresis with Electrospray Ionization Mass Spectrometry

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Clinical and Forensic Applications of Capillary Electrophoresis

Part of the book series: Pathology and Laboratory Medicine ((PLM))

Abstract

Since its first introduction in 1981 by Lucaks and Jorgenson (1,2), capillary electrophoresis (CE) has become a rapidly expanding analytical technique that has been successfully employed in a wide range of analytical areas, including pharmaceutical, agrochemical, and environmental fields and in clinical, pharmacological, and drug metabolism studies (3). The reasons for this fast breakthrough are the high efficiency, short analysis time, rapid method development, and simple instrumentation. Other advantages include the very small sample quantities (e.g., femtomoles) that can be analyzed. This is particularly important for studies in which sample size is limited. In addition, exotic and/or expensive background electrolyte (BGE) solutions can be used with minimal cost and disposal problems because of the low usage associated with CE compared to liquid chromatography. Because of the numerous publications in peer-reviewed journals, CE has been established as a method that is complementary to the more conventional separation techniques, such as high-performance liquid chromatography (HPLC) and gas chromatography (GC).

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  1. Samir Cherkaoui
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Cherkaoui, S. (2001). Combining Capillary Electrophoresis with Electrospray Ionization Mass Spectrometry. In: Petersen, J.R., Mohammad, A.A. (eds) Clinical and Forensic Applications of Capillary Electrophoresis. Pathology and Laboratory Medicine. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-120-6_15

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