Skip to main content
SpringerLink
Log in

Xenotransplantation of Human Cell Cultures in Nude Mice

  • Chapter
Tumor Models in Cancer Research

Part of the book series: Cancer Drug Discovery and Development ((CDD&D))

  • 449 Accesses

Abstract

The first successful xenotransplant of cultured human cells in nude mice was achieved by Giovanella et al. (1), who inoculated a suspension of melanoma cells subcutaneously in a nude mouse. This result was confirmed and extended by the same author (Giovanella et al. (2,3) and by others (4–9).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 74.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Giovanella BC, Yim SO, Stehlin JS, Williams LJ, Jr. Development of invasive tumors in the “nude” mouse after injection of cultured human melanoma cells. J Natl Cancer Inst 1972; 48: 1531–1533.

    PubMed  CAS  Google Scholar 

  2. Giovanella BC, Stehlin JS, Williams LJ. Heterotransplantation of human malignant tumors in “nude” mice. II. Malignant tumors induced by injection of cell cultures derived from human solid tumors. J Natl Cancer Inst 1974; 52: 921–930.

    PubMed  CAS  Google Scholar 

  3. Giovanella BC, Stehlin JS, Santamaria C, Yim SO, Morgan AC, Williams LJ Jr., et al. Human neoplastic and normal cells in tissue culture. I. Cell lines derived from malignant melanomas and normal melanocytes. J Natl Cancer Inst 1976; 55: 1131–1142.

    Google Scholar 

  4. Freedman V, Shin S. Cellular Tumorigenicity in nude mice: correlation with cell growth in semi-solid medium. Cell 1974; 3: 355–359.

    Article  PubMed  CAS  Google Scholar 

  5. Freedman V, Shin S. Isolation of human diploid cell variants with enhanced colony-forming efficiency in semi-solid medium after a single-stem chemical mutagenesis. J Natl Cancer Inst 1977; 58: 1873–1875.

    PubMed  CAS  Google Scholar 

  6. Freedman VH, Shin S. In: Fogh J, Giovanella B, eds. Use of Nude Mice for Studies on the Tumorigenicity of Animal Cells, Vol. 1. Academic Press, New York, NY, 1978, pp. 353–384.

    Google Scholar 

  7. Stiles D, Desmond W, Sato G, Saier M Jr. Failure of human cells transformed by simian virus 40 to form tumors in athymic nude mice. PNAS 1975; 72: 4971–4975.

    Article  PubMed  CAS  Google Scholar 

  8. Shin S, Freedman VH. Neoplastic growth of animal cells in nude mice. In: Nomura, T, Ohsawa N, Tamaoki N, Fujiwara K, eds. Proc. 2nd Int. Workshop on Nude Mice. pp. 337–349; U Tokyo Press, Tokyo, 1977.

    Google Scholar 

  9. Stiles CD, Kawahara AA. The Growth Behavior of Virus-Transformed Cells in Nude Mice. In: Fogh J, Giovanella BC, eds. The Nude Mouse in Experimental and Clinical Research. Academic Press, NY, 1978, pp. 385–409.

    Google Scholar 

  10. Giovanella, BC, Vardeman DM, Williams Li, Taylor DJ, PD, et al. Heterotransplantation of human breast carcinomas in nude mice: correlation between nude takes, poor prognosis and overexpression of the HER-2/neu Oncogene. Int J Cancer 1991; 47: 66–71.

    Google Scholar 

  11. Giovanella BC, Stehlin JS. Assessment of the Malignant Potential of Cultured Cells by Injection in “Nude” Mice. In: Rygaard J, Povlsen C, eds. Proceedings of the 1st International Workshop on “Nude” Mice. Fischer Verlag, Heidelburg, Germany 1974, pp. 279–284.

    Google Scholar 

  12. Giovanella BC, Fogh J. Present and future trends in investigations with the nude mouse as a recipient of human tumor transplants. In: Fogh J, Giovanella BC, eds. The Nude Mouse in Experimental and Clinical Research. Academic Press, New York, NY, 1978, pp. 282–312.

    Google Scholar 

  13. Stiles CD, Desmond W, Chuman LM, Sato G, Saier MH Jr. Growth control of heterologous tissue culture cells in the congenitally athymic nude mouse. Cancer Res 1976; 36: 1353–1360.

    PubMed  CAS  Google Scholar 

  14. Belewo J, Freedman VH, Shin S. Growth of murine sarcoma-virus transformed rat kidney cells in nude mice. J Natl Cancer Inst 1977; 58: 1691–1694.

    Google Scholar 

  15. Chen LB, Galimore PH, McDougall JD. Correlation between tumor induction and the large external transformation sensitive protein on the cell surface. PNAS 1976; 73: 3570–3574.

    Article  PubMed  CAS  Google Scholar 

  16. Giovanella BC, Stehlin JS, Yim SO. Establishment and characterization of a permanent cell line from a human teratocarcinoma. In Vitro 1974; 10: 382.

    Google Scholar 

  17. Zeuthen J, Norgaard JOR, Aner P, Fellous M, Wartiovaara J, Vaneri A, et al. Characterization of a human ovarian teratocarcinoma cell line. Int J Cancer 1980; 25: 19–32.

    Article  PubMed  CAS  Google Scholar 

  18. Tainsky MA, Cooper CS, Giovanella BC, Vande Woude GE An activated rasN gene: detected in late but not early passage human PA 1 teratocarcinoma cells. Science 1984; 225: 643–645.

    Article  PubMed  CAS  Google Scholar 

  19. Tainsky F, Shamanski D, Blair D, Giovanella BC. Causal Role for an Activated N-ras Oncogene in the Induction of Tumorigenicity Acquired by a Human Cell Line. Cancer Res 1987; 47: 3235–3238.

    PubMed  CAS  Google Scholar 

  20. Tainsky MA, Krizman DB, Chiao PJ, Yim SO, Giovanella BC. PA-1A human cell model for multistage carcinogenesis: oncogens and other factors. Anticancer Res 1988; 8: 899–914.

    PubMed  CAS  Google Scholar 

  21. Gaffney E, Polanowski FP, Blackburn SE, Lambiase JP. Origin, concentration and structural features of human mammary cells cultured from breast secretions. Cell Tissue Res 1976; 172: 269–279.

    Article  PubMed  CAS  Google Scholar 

  22. Giovanella BC, Gaffney EV, Yim SO, Stehlin JS. Increase of malignant potential of human line HBL 100 with long-term Passage in vitro. In Vitro 1980; 16: 211.

    Google Scholar 

  23. Gaffney EV. A cell line (HBL 100) established from breast milk. Cell tissue Res 1982; 227: 563–568.

    Article  PubMed  CAS  Google Scholar 

  24. Dhaliwal MK, Giovanella BC, Pathak S. Cytogenetic characterization of two human milk-derived cell line (HBL-100) passages differing in tumorigenicity. Anticancer Res 1990; 10: 113–118.

    PubMed  CAS  Google Scholar 

  25. Cailleau R, Olive MD, Cruciger QV. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 1978; 14: 911–915.

    Article  PubMed  CAS  Google Scholar 

  26. Cailleau R, Young R, Olive M, Reeves WJ, Jr. Breast tumor cell lines from pleural effusates. J Natl Cancer Inst 1974; 53: 661–674.

    PubMed  CAS  Google Scholar 

  27. Bischoff FZ, Yim SO, Pathak S, Grant G, Siciliano MJ, Giovanella BC, et al. Spontaneous abnormalities in normal fibroblasts from patients with Li-Fraumeni Cancer Syndrome: aneuploidy and immortalization. Cancer Res 1990; 50: 7979–7984.

    PubMed  CAS  Google Scholar 

  28. Nilsson K. Establishment of permanent human lymphoblastoid cell lines in vitro. In: David J, Bloom BR, eds. In Vitro Methods in Cell Mediated Tumor Immunity. Academic Press, New York, NY, 1976, pp. 713–721.

    Google Scholar 

  29. Nilsson K, Giovanella BC, Stehlin JS, Klein G. Tumorigenicity of human hematopoietic cell lines in athymic nude mice. Int J Cancer 1976; 19: 337–344.

    Article  Google Scholar 

  30. McCormick KJ, Giovanella BC, Klein G, Nilsson K, Stehlin JS. Diploid human lymphoblastoid and Burkitt lymphoma cell lines: susceptibility to murine NK cells and heterotransplantation to nude mice. Int J Cancer 1981; 28: 455–458.

    Article  PubMed  CAS  Google Scholar 

  31. Giovanella BC, Nilsson K, Zech L, Yim SO, Klein G, Stehlin JS. Growth of diploid, Epstein-Barr Virus carrying human lymphoblastoid cell lines heterotransplanted into nude mice under immunologically privileged conditions. Int J Cancer 1979; 24: 103–113.

    Article  PubMed  CAS  Google Scholar 

  32. Potmesil M, Vardeman D, Kozielski AJ, Mendoza J, Stehlin JS, Giovanella BC. Growth inhibition of human cancer metastases by camptothecins in newly developed xenograft models. Cancer Res 1995; 55: 5637–5641.

    PubMed  CAS  Google Scholar 

  33. Verschraegen C, Giovanella BC, Mendoza JT, Kozielski AJ, Stehlin JS, Jr. Specific organ metastases of human melanoma cells injected into the arterial circulation of nude mice. Anticancer Res 1991; 11: 529–536.

    PubMed  CAS  Google Scholar 

Download references

Authors
  1. Beppino Giovanella PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Lilly Research Laboratories, Indianapolis, IN, USA

    Beverly A. Teicher

Rights and permissions

Reprints and permissions

Copyright information

© 2002 Springer Science+Business Media New York

About this chapter

Cite this chapter

Giovanella, B. (2002). Xenotransplantation of Human Cell Cultures in Nude Mice. In: Teicher, B.A. (eds) Tumor Models in Cancer Research. Cancer Drug Discovery and Development. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-100-8_5

Download citation

  • DOI: https://doi.org/10.1007/978-1-59259-100-8_5

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4757-6883-1

  • Online ISBN: 978-1-59259-100-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation