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Biophysical and Biochemical Approaches in the Analysis of Argonaute–MicroRNA Complexes

  • Sujin Kim
  • Yoosik KimEmail author
Chapter
  • 460 Downloads
Part of the Biological and Medical Physics, Biomedical Engineering book series (BIOMEDICAL)

Abstract

MicroRNAs (miRNAs) are a class of small, noncoding, and single-stranded RNAs that play a key role in regulating gene expressions in most eukaryotes. Human genome encodes over 1,600 different miRNA loci, and miRNAs directly regulate expression of over 60% of protein-coding genes. The canonical miRNA biogenesis pathway has been well documented with key players identified by the efforts of many biochemical research groups. However, we still lack a mechanistic understanding of the target recognition and gene silencing processes. Over the recent years, structural, biochemical, and single-molecule biophysical studies have revealed the complexity in the interactions between miRNA and the effector protein Argonaute (Ago) as well as between Ago and the target mRNA. In this chapter, we present these findings that significantly enhanced our knowledge of the assembly and regulation by Ago–miRNA complexes.

Keywords

MicroRNA Argonaute RISC Protein structure Single-molecule experiment Speed–stability paradox 

Notes

Acknowledgements

This work was supported by Samsung Research Funding & Incubation Center of Samsung Electronics under Project Number SRFC-MA1702-08.

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Authors and Affiliations

  1. 1.Department of Chemical and Biomolecular EngineeringKAIST Institute for Health Science and Technology, KAISTDaejeonSouth Korea

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