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In Vitro Differentiation of Spermatogonia

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The Biology of Mammalian Spermatogonia

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Abstract

Presence of functional spermatogonial population secures continuing spermatogenesis, by repeated commencement of spermatogenic cycles from them. In vitro manipulation of spermatogonia, thus, can be divided into two aspects: proliferation of spermatogonia for their maintenance and initiation of their differentiation toward sperm formation. In this chapter, experiments on in vitro differentiation of spermatogonia using an organ culture technique are described. Principle of the method and results, using testis tissues of pup and adult mice as well as an infertile mutant mouse, are introduced. Spermatogenesis from isolated or cultured spermatogonia is also described. Finally, future challenges and prospects are stated.

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Authors and Affiliations

  1. Laboratory of Biopharmaceutical and Regenerative Sciences, Institute of Molecular Medicine and Life Science, Yokohama City University, 3-9 Fukuura, Kanazawa-ku, Yokohama, 236-0004, Japan

    Takehiko Ogawa M.D., Ph.D.

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  1. Takehiko Ogawa M.D., Ph.D.
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Correspondence to Takehiko Ogawa M.D., Ph.D. .

Editor information

Editors and Affiliations

  1. Centre for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, Washington, USA

    Jon M. Oatley

  2. School of Molecular Biosciences, Washington State University, Pullman, Washington, USA

    Michael D. Griswold

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Ogawa, T. (2017). In Vitro Differentiation of Spermatogonia. In: Oatley, J., Griswold, M. (eds) The Biology of Mammalian Spermatogonia. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-7505-1_12

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