Recording of Ionic Currents Under Physiological Conditions: Action Potential-Clamp and ‘Onion-Peeling’ Techniques

  • Ye Chen-IzuEmail author
  • Leighton T. Izu
  • Bence Hegyi
  • Tamás Bányász
Part of the Handbook of Modern Biophysics book series (HBBT, volume 5)


Upon stimulation, excitable cells generate a transient change in the membrane potential called Action Potential (AP). The AP is governed by numerous ionic currents that flow in or out of the cell membrane. The goal of cellular electrophysiology is to understand the role of individual ionic currents and the interplay between currents in determining the profile and time course of AP. A critically important question of the field is how the ionic currents behave individually and interact collectively during the AP cycle of an excitable cell? To answer this question we need to know the dynamic behavior of ionic currents during AP and how these currents work in concert to determine the cell’s membrane potential at every moment.


Ionic Current Pipette Solution Compensation Current Voltage Command Dynamic Clamp 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Ye Chen-Izu
    • 1
    Email author
  • Leighton T. Izu
    • 2
  • Bence Hegyi
    • 2
  • Tamás Bányász
    • 1
    • 3
  1. 1.Departments of Pharmacology, Biomedical Engineering, Internal Medicine/CardiologyUniversity of California, DavisDavisUSA
  2. 2.Department of PharmacologyUniversity of California, DavisDavisUSA
  3. 3.Department of PhysiologyUniversity of DebrecenDebrecenHungary

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