Abstract
While there have been many changes in the way HIV-1 protein research has been carried out since the advent of proteomic techniques, it is important to understand how we have arrived here and the basics of the methods that were used for critical early advances made by researchers who relied on rather primitive methods and alert minds. Indeed, many of the older techniques in their current form still to this day have many advantages over mass spectrometry methods and are often used to confirm and extend the results from high-throughput mass spectrometry. This chapter considers “HIV-1 proteomics” before there was proteomics, providing a short history of the retroviral biochemistry that has made so many vital contributions to our understanding of HIV-1 biology.
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Acknowledgments
This project has been funded in whole or in part with Federal funds from the National Cancer Institute, National Institutes of Health, under Contract No. HHSN261200800001E. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services; nor does the mention of trade names, commercial products, or organizations imply endorsement by the US government.
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Ott, D.E. (2016). 25 Years of HIV-1 Biochemistry. In: Graham, D., Ott, D. (eds) HIV-1 Proteomics. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-6542-7_3
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DOI: https://doi.org/10.1007/978-1-4939-6542-7_3
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