Fluorescence Spectroscopy: Basic Foundations and Methods
Fluorescence spectroscopy is a powerful experimental tool used by scientists from many disciplines. During the last decades there have been important developments on distinct fluorescence methods, particularly those related to the study of biological phenomena. This chapter discusses the foundations of the fluorescence phenomenon, introduces some general methodologies and provides selected examples on applications focused to disentangle structural and dynamical aspects of biological processes.
KeywordsLight absorption, fluorescence emission Fluorescence lifetime Fluorescence polarization FRET Fluorescence instrumentation Fluorophores Raster imaging correlation spectroscopy
The author wants to thanks the Danish National Research Foundation (which supports MEMPHYS-Center for Biomembrane Physics) and Drs. David Jameson and Roberto Stock for the critical reading of this manuscript.
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