Asymmetrical Flow Field Flow Fractionation: A Useful Tool for the Separation of Protein Pharmaceuticals and Particulate Systems

  • Julia EngertEmail author
  • Roman Mathaes
  • Gerhard Winter
Part of the Advances in Delivery Science and Technology book series (ADST)


The focus of this chapter will be on asymmetrical flow field flow fractionation (AF4) for the separation and characterization of protein pharmaceuticals and particulate systems. The chapter will provide some background and historical information on field flow fractionation and the general working principle. In addition, a practical guide on how to use AF4 will be described and critical parameters for the development of a suitable separation method will be discussed. The use of AF4 for protein pharmaceuticals as well as particulate systems will be described and some examples given in the literature will be presented. Finally, a summary of the most recent trends in AF4 and an outlook will be given for potential application fields in the future.


Asymmetrical flow field flow fractionation Protein pharmaceuticals Separation 



The authors wish to thank Dr.-Ing. Marcus Engert for preparing the schematic illustrations.


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Copyright information

© Controlled Release Society 2016

Authors and Affiliations

  1. 1.Department of Pharmacy, Pharmaceutical Technology and BiopharmaceuticsLudwig-Maximilians-University MunichMunichGermany

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