Abstract
The mitochondrial permeability transition is an inner membrane permeability increase that can be traced to the opening of a specific but nonselective high-conductance inner membrane channel named the permeability transition pore (PTP). PTP can open either transiently or permanently. There is an abundant literature from different laboratories using cellular or animal models, showing that PTP inhibition prevents some types of cell death. Although permanent PTP opening allows the release of mitochondrial pro-apoptotic proteins, the mechanism for release remains debated. Contrary to permanent PTP opening, transient PTP opening does not induce cell death and may have physiological functions. The best-characterized nonlethal function of PTP may be participation in calcium homeostasis, which is supported by limited but convincing evidence. Transient PTP opening may also participate in the phenomenon named ROS-induced ROS release and also serve in the uptake-release of compounds that lack a specific transport system in the inner membrane and are present both in matrix and cytosol at similar concentrations. Finally, transient PTP opening might allow ATP production by succinate-CoA ligase inside mitochondria when excess NADH inhibits the TCA cycle.
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Akerman KE (1978) Charge transfer during valinomycin-induced Ca2+ uptake in rat liver mitochondria. FEBS Lett 93:293–296
Alavian KN, Beutner G, Lazrove E, Sacchetti S, Park HA et al (2014) An uncoupling channel within the c-subunit ring of the F1FO ATP synthase is the mitochondrial permeability transition pore. Proc Natl Acad Sci U S A 111:10580–10585
Altschuld RA, Hohl CM, Castillo LC, Garleb AA, Starling RC et al (1992) Cyclosporin inhibits mitochondrial calcium efflux in isolated adult rat ventricular cardiomyocytes. Am J Physiol 262:H1699–H1704
Angelin A, Tiepolo T, Sabatelli P, Grumati P, Bergamin N et al (2007) Mitochondrial dysfunction in the pathogenesis of Ullrich congenital muscular dystrophy and prospective therapy with cyclosporins. Proc Natl Acad Sci U S A 104:991–996
Aprille JR (1988) Regulation of the mitochondrial adenine nucleotide pool size in liver: mechanism and metabolic role. FASEB J 2:2547–2556
Azzone GF, Azzi A (1965) Volume changes in liver mitochondria. Proc Natl Acad Sci U S A 53:1084–1089
Azzone GF, Pozzan T, Massari S, Bragadin M, Dell’Antone P (1977) H+/site ratio and steady state distribution of divalent cations in mitochondria. FEBS Lett 78:21–24
Baines CP, Kaiser RA, Purcell NH, Blair NS, Osinska H et al (2005) Loss of cyclophilin D reveals a critical role for mitochondrial permeability transition in cell death. Nature 434:658–662
Barsukova A, Komarov A, Hajnoczky G, Bernardi P, Bourdette D et al (2011) Activation of the mitochondrial permeability transition pore modulates Ca2+ responses to physiological stimuli in adult neurons. Eur J Neurosci 33:831–842
Basso E, Fante L, Fowlkes J, Petronilli V, Forte MA et al (2005) Properties of the permeability transition pore in mitochondria devoid of Cyclophilin D. J Biol Chem 280:18558–18561
Batandier C, Leverve X, Fontaine E (2004) Opening of the mitochondrial permeability transition pore induces reactive oxygen species production at the level of the respiratory chain complex I. J Biol Chem 279:17197–17204
Batandier C, Guigas B, Detaille D, El-Mir MY, Fontaine E et al (2006) The ROS production induced by a reverse-electron flux at respiratory-chain complex 1 is hampered by metformin. J Bioenerg Biomembr 38:33–42
Baughman JM, Perocchi F, Girgis HS, Plovanich M, Belcher-Timme CA et al (2011) Integrative genomics identifies MCU as an essential component of the mitochondrial calcium uniporter. Nature 476:341–345
Bernardi P, Azzone GF (1981) Cytochrome c as an electron shuttle between the outer and inner mitochondrial membranes. J Biol Chem 256:7187–7192
Bernardi P, Azzone GF (1982) A membrane potential-modulated pathway for Ca2+ efflux in rat liver mitochondria. FEBS Lett 139:13–16
Bernardi P, Azzone GF (1983) Regulation of Ca2+ efflux in rat liver mitochondria. Role of membrane potential. Eur J Biochem 134:377–383
Bernardi P, Petronilli V (1996) The permeability transition pore as a mitochondrial calcium release channel: a critical appraisal. J Bioenerg Biomembr 28:131–138
Bernardi P, von Stockum S (2012) The permeability transition pore as a Ca(2+) release channel: new answers to an old question. Cell Calcium 52:22–27
Bernardi P, Petronilli V, Di Lisa F, Forte M (2001) A mitochondrial perspective on cell death. Trends Biochem Sci 26:112–117
Boyer CS, Moore GA, Moldeus P (1993) Submitochondrial localization of the NAD+ glycohydrolase. Implications for the role of pyridine nucleotide hydrolysis in mitochondrial calcium fluxes. J Biol Chem 268:4016–4020
Bragadin M, Pozzan T, Azzone GF (1979) Kinetics of Ca2+ carrier in rat liver mitochondria. Biochemistry 18:5972–5978
Carraro M, Giorgio V, Sileikyte J, Sartori G, Forte M et al (2014) Channel formation by yeast F-ATP synthase and the role of dimerization in the mitochondrial permeability transition. J Biol Chem 289:15980–15985
Chauvin C, De Oliveira F, Ronot X, Mousseau M, Leverve X et al (2001) Rotenone inhibits the mitochondrial permeability transition-induced cell death in U937 and KB cells. J Biol Chem 276:41394–41398
Chouchani ET, Pell VR, Gaude E, Aksentijević D, Sundier SY et al (2014) Ischaemic accumulation of succinate controls reperfusion injury through mitochondrial ROS. Nature 515(7527):431–435
De Giorgi F, Lartigue L, Bauer MK, Schubert A, Grimm S et al (2002) The permeability transition pore signals apoptosis by directing Bax translocation and multimerization. FASEB J 16:607–609
De Stefani D, Raffaello A, Teardo E, Szabo I, Rizzuto R (2011) A forty-kilodalton protein of the inner membrane is the mitochondrial calcium uniporter. Nature 476:336–340
Detaille D, Guigas B, Chauvin C, Batandier C, Fontaine E et al (2005) Metformin prevents high-glucose-induced endothelial cell death through a mitochondrial permeability transition-dependent process. Diabetes 54:2179–2187
Di Lisa F, Ziegler M (2001) Pathophysiological relevance of mitochondria in NAD(+) metabolism. FEBS Lett 492:4–8
Di Lisa F, Menabo R, Canton M, Barile M, Bernardi P (2001) Opening of the mitochondrial permeability transition pore causes depletion of mitochondrial and cytosolic NAD+ and is a causative event in the death of myocytes in postischemic reperfusion of the heart. J Biol Chem 276:2571–2575
Dumas JF, Argaud L, Cottet-Rousselle C, Vial G, Gonzalez C et al (2009) Effect of transient and permanent permeability transition pore opening on NAD(P)H localization in intact cells. J Biol Chem 284:15117–15125
Elrod JW, Wong R, Mishra S, Vagnozzi RJ, Sakthievel B et al (2010) Cyclophilin D controls mitochondrial pore-dependent Ca(2+) exchange, metabolic flexibility, and propensity for heart failure in mice. J Clin Invest 120:3680–3687
Eriksson O, Pollesello P, Geimonen E (1999) Regulation of total mitochondrial Ca2+ in perfused liver is independent of the permeability transition pore. Am J Physiol 276:C1297–C1302
Fiermonte G, De Leonardis F, Todisco S, Palmieri L, Lasorsa FM et al (2004) Identification of the mitochondrial ATP-Mg/Pi transporter. Bacterial expression, reconstitution, functional characterization, and tissue distribution. J Biol Chem 279:30722–30730
Fontaine E, Eriksson O, Ichas F, Bernardi P (1998) Regulation of the permeability transition pore in skeletal muscle mitochondria. Modulation by electron flow through the respiratory chain complex i. J Biol Chem 273:12662–12668
Giorgio V, von Stockum S, Antoniel M, Fabbro A, Fogolari F et al (2013) Dimers of mitochondrial ATP synthase form the permeability transition pore. Proc Natl Acad Sci U S A 110:5887–5892
Guigas B, Detaille D, Chauvin C, Batandier C, De Oliveira F et al (2004) Metformin inhibits mitochondrial permeability transition and cell death: a pharmacological in vitro study. Biochem J 382:877–884
Haworth RA, Hunter DR (1979) The Ca2+-induced membrane transition in mitochondria. II. Nature of the Ca2+ trigger site. Arch Biochem Biophys 195:460–467
Haworth RA, Hunter DR (1980) Allosteric inhibition of the Ca2+-activated hydrophilic channel of the mitochondrial inner membrane by nucleotides. J Membr Biol 54:231–236
Hunter DR, Haworth RA (1979a) The Ca2+-induced membrane transition in mitochondria. I. The protective mechanisms. Arch Biochem Biophys 195:453–459
Hunter DR, Haworth RA (1979b) The Ca2+-induced membrane transition in mitochondria. III. Transitional Ca2+ release. Arch Biochem Biophys 195:468–477
Hunter DR, Haworth RA, Southard JH (1976) Relationship between configuration, function, and permeability in calcium-treated mitochondria. J Biol Chem 251:5069–5077
Irwin WA, Bergamin N, Sabatelli P, Reggiani C, Megighian A et al (2003) Mitochondrial dysfunction and apoptosis in myopathic mice with collagen VI deficiency. Nat Genet 35:367–371
Kantrow SP, Piantadosi CA (1997) Release of cytochrome c from liver mitochondria during permeability transition. Biochem Biophys Res Commun 232:669–671
Kinnally KW, Campo ML, Tedeschi H (1989) Mitochondrial channel activity studied by patch-clamping mitoplasts. J Bioenerg Biomembr 21:497–506
Kirichok Y, Krapivinsky G, Clapham DE (2004) The mitochondrial calcium uniporter is a highly selective ion channel. Nature 427:360–364
Korshunov SS, Skulachev VP, Starkov AA (1997) High protonic potential actuates a mechanism of production of reactive oxygen species in mitochondria. FEBS Lett 416:15–18
Kushnareva Y, Murphy AN, Andreyev A (2002) Complex I-mediated reactive oxygen species generation: modulation by cytochrome c and NAD(P)+ oxidation-reduction state. Biochem J 368:545–553
Kwong LK, Sohal RS (1998) Substrate and site specificity of hydrogen peroxide generation in mouse mitochondria. Arch Biochem Biophys 350:118–126
Lablanche S, Cottet-Rousselle C, Lamarche F, Benhamou PY, Halimi S et al (2011) Protection of pancreatic INS-1 beta-cells from glucose- and fructose-induced cell death by inhibiting mitochondrial permeability transition with cyclosporin A or metformin. Cell Death Dis 2:e134
Lamarche F, Carcenac C, Gonthier B, Cottet-Rousselle C, Chauvin C et al (2013) Mitochondrial permeability transition pore inhibitors prevent ethanol-induced neuronal death in mice. Chem Res Toxicol 26(1):78–88
Li B, Chauvin C, De Paulis D, De Oliveira F, Gharib A et al (2012) Inhibition of complex I regulates the mitochondrial permeability transition through a phosphate-sensitive inhibitory site masked by cyclophilin D. Biochim Biophys Acta 1817:1628–1634
Massari S, Azzone GF (1972) The equivalent pore radius of intact and damaged mitochondria and the mechanism of active shrinkage. Biochim Biophys Acta 283:23–29
Merlini L, Angelin A, Tiepolo T, Braghetta P, Sabatelli P et al (2008) Cyclosporin A corrects mitochondrial dysfunction and muscle apoptosis in patients with collagen VI myopathies. Proc Natl Acad Sci U S A 105:5225–5229
Mitchell P (1961) Coupling of phosphorylation to electron and hydrogen transfer by a chemi-osmotic type of mechanism. Nature 191:144–148
Nakagawa T, Shimizu S, Watanabe T, Yamaguchi O, Otsu K et al (2005) Cyclophilin D-dependent mitochondrial permeability transition regulates some necrotic but not apoptotic cell death. Nature 434:652–658
Palma E, Tiepolo T, Angelin A, Sabatelli P, Maraldi NM et al (2009) Genetic ablation of cyclophilin D rescues mitochondrial defects and prevents muscle apoptosis in collagen VI myopathic mice. Hum Mol Genet 18:2024–2031
Palty R, Silverman WF, Hershfinkel M, Caporale T, Sensi SL et al (2010) NCLX is an essential component of mitochondrial Na+/Ca2+ exchange. Proc Natl Acad Sci U S A 107:436–441
Petronilli V, Szabo I, Zoratti M (1989) The inner mitochondrial membrane contains ion-conducting channels similar to those found in bacteria. FEBS Lett 259:137–143
Petronilli V, Nicolli A, Costantini P, Colonna R, Bernardi P (1994) Regulation of the permeability transition pore, a voltage-dependent mitochondrial channel inhibited by cyclosporin A. Biochim Biophys Acta 1187:255–259
Petronilli V, Miotto G, Canton M, Brini M, Colonna R et al (1999) Transient and long-lasting openings of the mitochondrial permeability transition pore can be monitored directly in intact cells by changes in mitochondrial calcein fluorescence. Biophys J 76:725–734
Petronilli V, Penzo D, Scorrano L, Bernardi P, Di Lisa F (2001) The mitochondrial permeability transition, release of cytochrome c and cell death. Correlation with the duration of pore openings in situ. J Biol Chem 276:12030–12034
Pfeiffer DR, Kuo TH, Tchen TT (1976) Some effects of Ca2+, Mg2+, and Mn2+ on the ultrastructure, light-scattering properties, and malic enzyme activity of adrenal cortex mitochondria. Arch Biochem Biophys 176:556–563
Piot C, Croisille P, Staat P, Thibault H, Rioufol G et al (2008) Effect of cyclosporine on reperfusion injury in acute myocardial infarction. N Engl J Med 359:473–481
Raaflaub J (1953a) Mechanism of adenosinetriphosphate as cofactor of isolated mitochondria. Helv Physiol Pharmacol Acta 11:157–165
Raaflaub J (1953b) Swelling of isolated mitochondria of the liver and their susceptibility to physicochemical influences. Helv Physiol Pharmacol Acta 11:142–156
Scarpa A, Azzone GF (1970) The mechanism of ion translocation in mitochondria. 4. Coupling of K+ efflux with Ca2+ uptake. Eur J Biochem 12:328–335
Schinzel AC, Takeuchi O, Huang Z, Fisher JK, Zhou Z et al (2005) Cyclophilin D is a component of mitochondrial permeability transition and mediates neuronal cell death after focal cerebral ischemia. Proc Natl Acad Sci U S A 102:12005–12010
Scorrano L, Ashiya M, Buttle K, Weiler S, Oakes SA et al (2002) A distinct pathway remodels mitochondrial cristae and mobilizes cytochrome c during apoptosis. Dev Cell 2:55–67
Szabo I, Zoratti M (1991) The giant channel of the inner mitochondrial membrane is inhibited by cyclosporin A. J Biol Chem 266:3376–3379
Tiepolo T, Angelin A, Palma E, Sabatelli P, Merlini L et al (2009) The cyclophilin inhibitor Debio 025 normalizes mitochondrial function, muscle apoptosis and ultrastructural defects in Col6a1-/- myopathic mice. Br J Pharmacol 157:1045–1052
von Stockum S, Giorgio V, Trevisan E, Lippe G, Glick GD et al (2015) F-ATPase of Drosophila melanogaster forms 53-picosiemen (53-pS) channels responsible for mitochondrial Ca2+-induced Ca2+ release. J Biol Chem 290:4537–4544
Votyakova TV, Reynolds IJ (2001) DeltaPsi(m)-Dependent and -independent production of reactive oxygen species by rat brain mitochondria. J Neurochem 79:266–277
Wang X, Carlsson Y, Basso E, Zhu C, Rousset CI et al (2009) Developmental shift of cyclophilin D contribution to hypoxic-ischemic brain injury. J Neurosci 29:2588–2596
Zoratti M, Szabo I (1995) The mitochondrial permeability transition. Biochim Biophys Acta 1241:139–176
Zorov DB, Filburn CR, Klotz LO, Zweier JL, Sollott SJ (2000) Reactive oxygen species (ROS)-induced ROS release: a new phenomenon accompanying induction of the mitochondrial permeability transition in cardiac myocytes. J Exp Med 192:1001–1014
Zorov DB, Juhaszova M, Sollott SJ (2014) Mitochondrial reactive oxygen species (ROS) and ROS-induced ROS release. Physiol Rev 94:909–950
Zulian A, Rizzo E, Schiavone M, Palma E, Tagliavini F et al (2014) NIM811, a cyclophilin inhibitor without immunosuppressive activity, is beneficial in collagen VI congenital muscular dystrophy models. Hum Mol Genet 23:5353–5363
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Fontaine, E., Bernardi, P. (2016). Lethal and Nonlethal Functions of the Permeability Transition Pore. In: Hockenbery, D. (eds) Mitochondria and Cell Death. Cell Death in Biology and Diseases. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3612-0_1
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DOI: https://doi.org/10.1007/978-1-4939-3612-0_1
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