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Molecular Mechanisms of Apical and Basolateral Sorting in Polarized Epithelial Cells

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Ion Channels and Transporters of Epithelia in Health and Disease

Part of the book series: Physiology in Health and Disease ((PIHD))

Abstract

The proper localization of ion channels and transporters to the apical or basolateral membrane domains is essential for correct ion transport through epithelia. Polarized sorting is achieved through elaborate intracellular sorting pathways encompassing the trans-Golgi network, early endosomes, and recycling endosomes that are fine-tuned for epithelial function and thus different from the secretory and endocytic systems in non-polarized cells. Polarized cells express epithelial cell-specific molecules, including sorting adaptors like the cytosolic clathrin adaptor AP-1B, that are required for efficient segregation and targeting of proteins to specific plasma membrane domains. In this book chapter, we will first discuss the various sorting stations in the cells followed by a review of apical and basolateral sorting signals and their interpretation at different compartments. Specific emphasis is placed on the trafficking of ion transporters.

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Abbreviations

AEE:

Apical early endosome

ARE:

Apical RE

BEE:

Basolateral early endosome

CRE:

Common RE

RE:

Recycling endosome

TGN:

trans-Golgi network

TJ:

Tight junction

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Acknowledgments

We apologize to colleagues whose work we could not cite due to space constraints. Work in the Weisz lab is supported by NIH R01 DK100357, and work in the Fölsch lab is supported by NIH R01 GM0707036.

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Authors and Affiliations

  1. Renal-Electrolyte Division, Department of Medicine, University of Pittsburgh, 3550 Terrace Street, Pittsburgh, 15261, PA, USA

    Ora A. Weisz

  2. Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, 303 East Chicago Avenue, Chicago, IL, 60611, USA

    Heike Fölsch

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  1. Ora A. Weisz
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  2. Heike Fölsch
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Correspondence to Heike Fölsch .

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Editors and Affiliations

  1. Department of Physiology, University of Otago , Dunedin, Otago, New Zealand

    Kirk L. Hamilton

  2. Department of Cell Biology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA

    Daniel C Devor

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Weisz, O.A., Fölsch, H. (2016). Molecular Mechanisms of Apical and Basolateral Sorting in Polarized Epithelial Cells. In: Hamilton, K., Devor, D. (eds) Ion Channels and Transporters of Epithelia in Health and Disease. Physiology in Health and Disease. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-3366-2_7

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