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Aging Effect on Post-recovery Hypofusion and Mortality Following Cardiac Arrest and Resuscitation in Rats

  • Kui XuEmail author
  • Michelle A. Puchowicz
  • Joseph C. LaManna
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 876)

Abstract

In this study we investigated the effect of aging on brain blood flow following transient global ischemia. Male Fisher rats (6 and 24 months old) underwent cardiac arrest (15 min) and resuscitation. Regional brain (cortex, hippocampus, brainstem and cerebellum) blood flow was measured in non-arrested rats and 1-h recovery rats using [14C] iodoantipyrene (IAP) autoradiography; the 4-day survival rate was determined in the two age groups. The pre-arrest baseline blood flows were similar in cortex, brainstem and cerebellum between the 6-month and the 24-month old rats; however, the baseline blood flow in hippocampus was significantly lower in the 24-month old group. At 1 h following cardiac arrest and resuscitation, both 6-month and 24-month groups had significantly lower blood flows in all regions than the pre-arrest baseline values; compared to the 6-month old group, the blood flow was significantly lower (about 40 % lower) in all regions in the 24-month old group. The 4-day survival rate for the 6-month old rats was 50 % (3/6) whereas none of the 24-month old rats (0/10) survived for 4 days. The data suggest that there is an increased vulnerability to brain ischemic-reperfusion injury in the aged rats; the degree of post-recovery hypoperfusion may contribute to the high mortality in the aged rats following cardiac arrest and resuscitation.

Keywords

Transient global ischemia Cerebral blood flow Ischemia/reperfusion injury Aged rat Survival 

Notes

Acknowledgments

This study was supported by NIH grant NINDS 1 R01 NS46074.

References

  1. 1.
    Zheng ZJ, Croft JB, Giles WH et al (2001) Sudden cardiac death in the United States, 1989 to 1998. Circulation 104:2158–2163CrossRefPubMedGoogle Scholar
  2. 2.
    Gidday JM, Kim YB, Shah AR et al (1996) Adenosine transport inhibition ameliorates postischemic hypoperfusion in pigs. Brain Res 734:261–268CrossRefPubMedGoogle Scholar
  3. 3.
    Adrie C, Adib-Conquy M, Laurent I et al (2002) Successful cardiopulmonary resuscitation after cardiac arrest as a “sepsis-like” syndrome. Circulation 106:562–568CrossRefPubMedGoogle Scholar
  4. 4.
    Xu K, Puchowicz MA, Lust WD et al (2006) Adenosine treatment delays postischemic hippocampal CA1 loss after cardiac arrest and resuscitation in rats. Brain Res 1071:208–217CrossRefPubMedGoogle Scholar
  5. 5.
    Xu K, Puchowicz MA, Sun X et al (2010) Decreased brainstem function following cardiac arrest and resuscitation in aged rat. Brain Res 1328:181–189CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Xu K, LaManna JC (2009) The loss of hypoxic ventilatory responses following resuscitation after cardiac arrest in rats is associated with failure of long-term survival. Brain Res 1258:59–64CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Palkovits M, Brownstein MJ (1988) Maps and guide to microdissection of rat brain. Elsevier, New YorkGoogle Scholar
  8. 8.
    Negovsky VA, Gurvitch AM (1995) Post-resuscitation disease--a new nosological entity. Its reality and significance. Resuscitation 30:23–27CrossRefPubMedGoogle Scholar
  9. 9.
    Mortberg E, Cumming P, Wiklund L et al (2009) Cerebral metabolic rate of oxygen (CMRO2) in pig brain determined by PET after resuscitation from cardiac arrest. Resuscitation 80:701–706CrossRefPubMedGoogle Scholar
  10. 10.
    Oku K, Kuboyama K, Safar P et al (1994) Cerebral and systemic arteriovenous oxygen monitoring after cardiac arrest. Inadequate cerebral oxygen delivery. Resuscitation 27:141–152CrossRefPubMedGoogle Scholar
  11. 11.
    Sterz F, Leonov Y, Safar P et al (1992) Multifocal cerebral blood flow by Xe-CT and global cerebral metabolism after prolonged cardiac arrest in dogs. Reperfusion with open-chest CPR or cardiopulmonary bypass. Resuscitation 24:27–47CrossRefPubMedGoogle Scholar
  12. 12.
    Aanerud J, Borghammer P, Chakravarty MM et al (2012) Brain energy metabolism and blood flow differences in healthy aging. J Cereb Blood Flow Metab 32:1177–1187CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media, New York 2016

Authors and Affiliations

  • Kui Xu
    • 1
    Email author
  • Michelle A. Puchowicz
    • 2
  • Joseph C. LaManna
    • 1
  1. 1.Department of Physiology and BiophysicsCase Western Reserve UniversityClevelandUSA
  2. 2.Departments of NutritionCase Western Reserve UniversityClevelandUSA

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