Prototyping the Experimental Setup to Quantify the Tissue Oxygen Consumption Rate and Its Preliminary Test

  • N. WatanabeEmail author
  • M. Shibata
  • S. Sawada
  • K. Mizukami
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 876)


In order to establish a reliable and practical method to make a diagnosis on the viability of an amputated extremity, we propose a method to evaluate the oxygen consumption rate. To validate this concept, we prototyped an experimental system with which the oxygen transfer rate into tissue can be assessed by the rate of change of the decrease in dissolved oxygen (DO) concentration within the buffer fluid surrounding the target tissue. The purpose of this study is to examine the feasibility of our prototyped experimental system by comparison between fresh and non-fresh rat skeletal muscles. The results show that the fresher tissue transferred more oxygen to the tissue, which suggests that tissue oxygen consumption is highly related to tissue freshness and can indirectly assess the tissue viability.


Oxygendiffusion Dissolved oxygen Tissue freshness Tissue viability Temperature 



A part of this paper was reported in the Master’s thesis of S. Sawada [9] at Shibaura Institute of Technology, Japan (2013).


  1. 1.
    Levick JR (2010) An introduction to cardiovascular physiology, 5th edn. Hodder, Arnold and Hachette, London. ISBN 9780340942048Google Scholar
  2. 2.
    Hynes J, Floyd S, Soini AE, O’Connor R, Papkovsky DB (2003) Fluorescence-based cell viability screening assays using water-soluble oxygen probes. J Biomol Screen 8(3):264–272CrossRefPubMedGoogle Scholar
  3. 3.
    Dmitriev RI, Papkovsky DB (2012) Optical probes and techniques for O2 measurement in live cells and tissue. Cell Mol Life Sci 69:2025–2039CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Fry FEJ, Hart JS (1948) The relation of temperature to oxygen consumption in the goldfish. Biol Bull 94(1):66–77CrossRefPubMedGoogle Scholar
  5. 5.
    Saito A (2003) Skeletal muscle structure. Rigakuryoho Kagaku 18(1):49–53CrossRefGoogle Scholar
  6. 6.
    Mottram RF (1955) The oxygen consumption of human skeletal muscle in vivo. J Physiol 128(2):268–276CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Reyes AB, Pendergast JS, Yamazaki S (2008) Mammalian peripheral circadian oscillators are temperature compensated. J Biol Rhythms 23:95–98CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Schmidt-Nielsen K (1997) Animal physiology adaptation and environment, 5th edn. Cambridge University Press, Cambridge, p 220Google Scholar
  9. 9.
    Sawada S (2014) Development of experimental setup to measure the tissue oxygen consumption. Master’s thesis of Shibaura Institute of Technology (in Japanese)Google Scholar

Copyright information

© Springer Science+Business Media, New York 2016

Authors and Affiliations

  • N. Watanabe
    • 1
    • 2
    Email author
  • M. Shibata
    • 1
    • 2
  • S. Sawada
    • 2
  • K. Mizukami
    • 1
  1. 1.Department of Bioscience and EngineeringCollege of Systems Engineering and Science, Shibaura Institute of TechnologySaitamaJapan
  2. 2.Systems Engineering and Science, Graduate school of Engineering and ScienceShibaura Institute of TechnologySaitamaJapan

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