Direct Evidence of the Link Between Energetic Metabolism and Proliferation Capacity of Cancer Cells In Vitro

  • Géraldine De Preter
  • Pierre Danhier
  • Paolo E. Porporato
  • Valéry L. Payen
  • Bénédicte F. Jordan
  • Pierre Sonveaux
  • Bernard GallezEmail author
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 876)


The aim of the study was to assess the link between the metabolic profile and the proliferation capacity of a range of human and murine cancer cell lines. First, the combination of mitochondrial respiration and glycolytic efficiency measurements allowed the determination of different metabolic profiles among the cell lines, ranging from a mostly oxidative to a mostly glycolytic phenotype. Second, the study revealed that cell proliferation, evaluated by DNA synthesis measurements, was statistically correlated to glycolytic efficiency. This indicated that glycolysis is the key energetic pathway linked to cell proliferation rate. Third, to validate this hypothesis and exclude non-metabolic factors, mitochondria-depleted were compared to wild-type cancer cells, and the data showed that enhanced glycolysis observed in mitochondria-depleted cells is also associated with an increase in proliferation capacity.


Cancer metabolism Proliferation Electron paramagnetic resonance Oxidative phosphorylation Aerobic glycolysis 



This work was supported by grants from the Télévie, the Belgian National Fund for Scientific Research (F.R.S.-FNRS), the Fonds Joseph Maisin, the Fondation Belge contre le Cancer, the Saint-Luc Foundation, the Actions de Recherches Concertées-Communauté Française de Belgique (ARC 04/09-317), and the European Research Council (FP7/2007-2013 ERC Independent Researcher Starting Grant No. 243188 TUMETABO). B.F.J. and P.S. are Research Associates and P.D. a Research Fellow of the F.R.S.-FNRS. G.D. is a Télévie Research Fellow.


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Copyright information

© Springer Science+Business Media, New York 2016

Authors and Affiliations

  • Géraldine De Preter
    • 1
  • Pierre Danhier
    • 1
  • Paolo E. Porporato
    • 2
  • Valéry L. Payen
    • 2
  • Bénédicte F. Jordan
    • 1
  • Pierre Sonveaux
    • 2
  • Bernard Gallez
    • 1
    Email author
  1. 1.Biomedical Magnetic Resonance Research GroupUniversité catholique de Louvain (UCL)BrusselsBelgium
  2. 2.Institut de Recherche Expérimentale et Clinique (IREC)Pole of Pharmacology, Université catholique de Louvain (UCL)BrusselsBelgium

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