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Tissue Discs: A 3D Model for Assessing Modulation of Tissue Oxygenation

  • M. J. Gandolfo
  • A. H. Kyle
  • A. I. MinchintonEmail author
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 876)

Abstract

The presence of hypoxia in solid tumours is correlated with poor treatment outcome. We have developed a 3-D tissue engineered construct to quantitatively monitor oxygen penetration through tumour tissue using the exogenous 2-nitroimidazole bioreductive probe pimonidazole and phosphorescence quenching technologies. Using this in vitro model we were able to examine the influence of the biguanides metformin and phenformin, antimycin A and KCN, on the distribution and kinetics of oxygen delivery as prototypes of modulators of oxygen metabolism.

Keywords

Hypoxia Oxygensensing Tumour Metabolism Radiosensitivity 

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Copyright information

© Springer Science+Business Media, New York 2016

Authors and Affiliations

  • M. J. Gandolfo
    • 1
  • A. H. Kyle
    • 1
  • A. I. Minchinton
    • 1
    Email author
  1. 1.Department of Integrative OncologyBC Cancer Research CentreVancouverCanada

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