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Myocardial Microcirculation and Mitochondrial Energetics in the Isolated Rat Heart

  • J. F. AshrufEmail author
  • C. InceEmail author
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 876)

Abstract

Normal functioning of myocardium requires adequate oxygenation, which in turn is dependent on an adequate microcirculation. NADH-fluorimetry enables a direct evaluation of the adequacy of tissue oxygenation while the measurement of quenching of Pd-porphyrine (PpIX) phosphorescence enables quantitative measurement of microvascular pO2. Combination of these two techniques provides information about the relation between microvascular oxygen content and parenchymal oxygen availability in Langendorff hearts. In normal myocardium there is heterogeneity at the microcirculatory level resulting in the existence of microcirculatory weak units, originating at the capillary level, which reoxygenate the slowest upon reoxygenation after an episode of ischemia. Sepsis and myocardial hypertrophia alter the pattern of oxygen transport whereby the microcirculation is disturbed at the arteriolar/arterial level. NADH fluorimetry also reveals a disturbance of mitochondrial oxygen availability in sepsis. Furthermore it is shown that these techniques can also be applied to various organs and tissues in vivo.

Keywords

Microcirculation Mitochondrial redox state NADH fluorescence Sepsis Hypertrophia 

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Copyright information

© Springer Science+Business Media, New York 2016

Authors and Affiliations

  1. 1.Department of SurgeryOZG HospitalGroningenThe Netherlands
  2. 2.Department of Translational PhysiologyAcademic Medical Center, University of AmsterdamAmsterdamThe Netherlands

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