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Brassica Ogu-INRA Cytoplasmic Male Sterility: An Example of Successful Plant Somatic Fusion for Hybrid Seed Production

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Somatic Genome Manipulation

Abstract

This chapter retraces the history of using somatic hybridization to modify the cytoplasmic male sterility (CMS)-inducing Ogura radish cytoplasm and its application in hybrid seed production for Brassica crops. First, we present hybrid seed production and the issues behind the introduction of the Ogura radish cytoplasm into Brassica crops. We then detail the procedures of the protoplast fusion experiments used to produce cybrids. Recombination between radish and Brassica mitochondrial genomes ultimately led to the creation of the Ogu-INRA system. Not only have cybrids contributed to hybrid seed production but also molecular analysis of cybrids helped identify the mitochondrial gene causing CMS, and contributed basic knowledge on plant mitochondrial gene expression. Furthermore, restoration of male fertility is useful for some crops, and the Ogu-INRA system has also been used to identify restorer genes. The Ogu-INRA system is now widely used in agriculture.

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Acknowledgments

We are very grateful to our colleagues at the INRA Rennes Center for their very impressive work on rapeseed breeding and for our long-running and very fruitful collaboration, particularly with Michel Renard and Régine Delourme. We acknowledge the excellent contributions of all colleagues and students involved in Versailles and Rennes INRA Centers who have worked for over 30 years on the Ogu-INRA CMS in Brassica. Our research on this CMS system has been partly funded by PROMOSOL, CETIOM, and Genoplante. We are also very grateful to Céline Duroc, joint head of the CETIOM technical center, for her help in retrieving data on the use of the Ogu-INRA system in agriculture.

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  1. Institut Jean-Pierre Bourgin, INRA-AgroParisTech UMR 1318, Versailles, France

    Dr. Georges Pelletier & Dr. Francoise Budar

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  1. Dr. Georges Pelletier
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  2. Dr. Francoise Budar
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Correspondence to Georges Pelletier .

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Editors and Affiliations

  1. Potato Research Centre, Agriculture and Agri-Food Canada, Government of Canada, Fredericton, New Brunswick, Canada

    Xiu-Qing Li

  2. Plant Science Department, McGill University, Sainte-Anne-de-Bellevue, Québec, Canada

    Danielle J. Donnelly

  3. Department of Biomedicine, Aarhus University, Herning, Denmark

    Thomas G. Jensen

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Pelletier, G., Budar, F. (2015). Brassica Ogu-INRA Cytoplasmic Male Sterility: An Example of Successful Plant Somatic Fusion for Hybrid Seed Production. In: Li, XQ., Donnelly, D., Jensen, T. (eds) Somatic Genome Manipulation. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-2389-2_9

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