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Nonlinear Microscopy of the Vocal Folds

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Biomedical Optics in Otorhinolaryngology

Abstract

Nonlinear microscopy is becoming a very important tool available to life scientists. This powerful three-dimensional technique allows exploration of unstained biological tissues through a contrast provided by the nonlinear interaction of short laser pulses with certain macromolecules such as elastin and collagen. The possibility of imaging microstructures (cells, nuclei) as well as macromolecules without affecting the integrity of the organ paves the way for a better understanding of vocal folds’ normal and pathological conditions. In this chapter, we review the physical concepts behind nonlinear microscopy and provide example of its use in laryngology.

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Notes

  1. 1.

    The optimal 2PEF excitation wavelength is typically broader allowing for excitation wavelengths to range from 700 to 1000 nm. This effect is due to the difference in allowed transition between sublevels of the ground and excited states when excited with one or two photons.

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Acknowledgments

The authors would like to thank Dr Amber Beckley, Mr. Étienne de Montigny, Ms. Chloé Gariépy, Mr. Scott Infusino, Pr. Steven Maturo, and Dr. Shilpa Ojha for fruitful discussions. Pr. Boudoux acknowledges funding from the National Sciences and Engineering Research Council of Canada (NSERC) and the Canadian Foundation for Innovation (CFI).

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Authors and Affiliations

  1. Department of Engineering Physics, Ecole Polytechnique Montréal, Montreal, QC, H3T 1J4, Canada

    Mathias Strupler PhD, Nadir Goulamhoussen MScA & Caroline Boudoux PhD

  2. Department of Biomedical Engineering, Ecole Polytechnique, Montreal, QC, Canada

    Romain Deterre MScA & Fouzi Benboujja MScA

  3. Department of Otolaryngology, Harvard Medical School, Massachusetts Eye and Ear Infirmary, Boston, MA, USA

    Christopher J. Hartnick MD, MS

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  1. Mathias Strupler PhD
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  6. Caroline Boudoux PhD
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Correspondence to Caroline Boudoux PhD .

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Editors and Affiliations

  1. Department of Otolaryngology Head and Neck Surgery, Department of Biomedical Engineering, Division of Facial Plastic Surgery, The Beckman Laser Institute and Medical Clinic, University of California Irvine, Irvine, California, USA

    Brian J.-F. Wong

  2. Department of Otorhinolaryngology, Plastic, Head and Neck Surgery, RWTH Aachen University Hospital, Aachen, Germany

    Justus Ilgner

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Strupler, M., Deterre, R., Goulamhoussen, N., Benboujja, F., Hartnick, C.J., Boudoux, C. (2016). Nonlinear Microscopy of the Vocal Folds. In: Wong, BF., Ilgner, J. (eds) Biomedical Optics in Otorhinolaryngology. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1758-7_31

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