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S-layer Structure in Bacteria and Archaea

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Nanomicrobiology

Abstract

In most archaeons and many bacteria, the cell surface is covered by a monomolecular and paracrystalline protein layer, called S-layer. S-layers serve as an outer protective shell and also act as molecular sieves and/or as cell-adhesion sites for exoenzymes. In bacteria, S-layers are associated either with peptidoglycan or with secondary cell wall polymers of the outer membrane. In archaea, the S-layer proteins (SLPs) are anchored to the cytoplasmic membrane and form the major cell wall component. A prominent feature of SLPs is their self-assembly into mono- or bilayers in solution or at interfaces. The unique self-assembling feature of S-layers provides the basis for a wide range of applications in biotechnology and nanobiology. This chapter covers the structural aspects of SLPs and S-layer assembly.

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Authors and Affiliations

  1. Departments of Structural and Molecular Microbiology, Structural Biology Research Center, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium

    Chaithanya Madhurantakam & Han Remaut

  2. Department of Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium

    Chaithanya Madhurantakam & Han Remaut

  3. Department of Chemistry, Institute of Structural and Molecular Biology, University College London, WC1H 0AJ, London, UK

    Stefan Howorka

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  1. Chaithanya Madhurantakam
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  2. Stefan Howorka
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  3. Han Remaut
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  1. Department of Biology, University of New Mexico, Albuquerque, New Mexico, USA

    Larry L. Barton

  2. School of Life Sciences, University of Nevada at Las Vegas, Las Vegas, Nevada, USA

    Dennis A. Bazylinski

  3. Department of Geoscience, University of Wisconsin - Madison, Madison, Wisconsin, USA

    Huifang Xu

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Madhurantakam, C., Howorka, S., Remaut, H. (2014). S-layer Structure in Bacteria and Archaea. In: Barton, L., Bazylinski, D., Xu, H. (eds) Nanomicrobiology. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1667-2_2

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