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Humanized Mice for HIV Research pp 83–90Cite as

Improvement of Human Multilineage Hematopoietic Engraftment by Cytokine Knock-in Replacement in Human‐Hemato‐Lymphoid System Mice

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Abstract

Mice carrying a human hemato-lymphoid system (HHLS mice) represent a valuable tool to study human immunity, inflammation, and hematopoiesis in in vivo settings. However, in currently available models of HHLS mice, the development and/or function of many human immune cell types is suboptimal. This condition is mainly due to a reduced or absent cross-reactivity of cytokines produced by the mouse host on the human cells. Here, we describe the different methods by which human cytokines can be delivered in HHLS mice in order to support human hematopoiesis and immune function. In particular, we focus our discussion on genetically modified host mice in which mouse cytokines are replaced by their human counterparts.

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Author information

Authors and Affiliations

  1. Department of Immunobiology, Yale University, 06520, New Haven, CT, USA

    Anthony Rongvaux & Richard A. Flavell

  2. Howard Hughes Medical Institute,, Yale University, 06520, New Haven, CT, USA

    Richard A. Flavell

  3. Division of Hematology, University Hospital Zürich, 8091, Zurich, Switzerland

    Markus G. Manz

Authors
  1. Anthony Rongvaux
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  2. Markus G. Manz
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  3. Richard A. Flavell
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Corresponding author

Correspondence to Richard A. Flavell .

Editor information

Editors and Affiliations

  1. Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, Nebraska, USA

    Larisa Y. Poluektova

  2. Center for AIDS Research, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA

    J. Victor Garcia

  3. Research Center for AIDS Laboratory of Viral Pathogenesis, Kyoto University Institute for Virus Research, Sakyo-ku, Japan

    Yoshio Koyanagi

  4. Division of Hematology, University Hospital Zürich, Zürich, Switzerland

    Markus G. Manz

  5. Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, USA

    Andrew M. Tager

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Rongvaux, A., Manz, M., Flavell, R. (2014). Improvement of Human Multilineage Hematopoietic Engraftment by Cytokine Knock-in Replacement in Human‐Hemato‐Lymphoid System Mice. In: Poluektova, L., Garcia, J., Koyanagi, Y., Manz, M., Tager, A. (eds) Humanized Mice for HIV Research. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1655-9_8

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